芦笋中毒死蜱和氯氰菊酯残留的同时测定

采用乙腈提取,Florisil固相萃取,正己烷-丙酮洗脱,HP-5石英柱程序升温技术分离,电子捕获检测器检测,建立了同时测定芦笋中毒死蜱和氯氰菊酯残留量的固相萃取-毛细管气相色谱法。结果表明,该方法对2种农药残留量的线性范围分别为0.002 5-0.500 0 mg/L和0.005-0.250 mg/L,相关系数为0.999 8-0.999 9,检出限分别为0.002 5 mg/kg和0.005 mg/kg,加标平均回收率为74.0%-98.3%,相对标准偏差为0.6%-10.8%。     

长豇豆新品种‘丰产6号’

 长豇豆早中熟新品种‘丰产6号’是由‘增城花仁白豆’的变异株系统选育而成。荚色绿白,荚条直,肉厚,荚长60 cm,荚宽1.0 cm,单荚质量28 g。荚形整齐,荚面平整,纤维少,品质优,产量高,对光周期反应不敏感,田间枯萎病、锈病发病率低,适宜广东省春夏秋季种植。     

芦笋茎枯病病原菌及其生物学研究

芦笋茎枯病是一种分布广、为害重的毁灭性病害。研究证实,临沂市芦笋茎枯病病原为Phoma asparagi Sacc.。该菌分生孢子发芽和菌丝生长适温为23—26℃,致死温度分别为50℃10分钟和60℃10分钟,发芽和生长最适pH为7。分生孢子生长在分生孢子器内,单个分生孢子常温下可活10天,菌块内可活25天,孢子器内则长达240天。分生孢子在蒸馏水中不发芽,加糖液发芽率略有提高,在文竹和芦笋茎煮液中发芽率最高;条件适宜时2小时产生芽突,5小时发芽率达98.21%。     

5%氟啶脲乳油防治甜菜夜蛾效果研究

用氟啶脲（ｃｈｌｏｒｆｌｕａｚｕｒｏｎ）溶液处理甜菜液蛾（Ｌａｐｈｙｇｍａ ｅｘｉｇｕａ Ｈｕｂｎｅｒ）不同日龄卵和不同龄期幼虫,结果发展,氟啶脲对１日龄卵有一定直接杀灭作用,对２￣３日龄卵直接杀伤效果低,随着龄期的增加。幼虫对氟啶脲的敏感性降低,卵盛期１５￣２５ｍＬ／６６７ｍ＾２（制剂用量）,２￣３龄幼虫盛发期３０￣６０ｍＬ／６６７ｍ＾２（制剂用量）,均可较好的控制甜菜夜蛾的危害,且持效期达１５     

矮生长豇豆的生育与产量形成

对春播和秋播矮生长豇豆的生长动态,叶面积形成与效能,结荚与豆荚产量形成特性等进行研究,结果表明,春播矮生长豇豆植株和各个器官的生长动态均呈Ｓ形曲线变化。秋播矮生长豇豆虽然无抽蔓期,但其生长动态与春播基本相同,而且植株生长和叶面积都较大,单株结荚稍多,但产量无明显差异。净同化率（ＮＡＲ）在营养生长过程较高,生殖生长过程较低。本文还讨论了矮生与蔓生长豇豆生育与产量形成的差异。     

缺磷胁迫对长豇豆幼苗脱落酸含量的影响

研究耐缺磷程度不同的长豇豆品种在缺磷胁迫下脱落酸(ABA)含量的变化。结果表明缺磷胁迫下长豇豆幼苗根系ABA含量都增高,缺磷前期下降后升至1个高峰后再降低,高峰出现的迟早与品种耐缺磷性有密切关系,不耐缺磷的二芦白比耐缺磷的芦花白出现早,平均升幅香港青二芦白芦花白。缺磷胁迫下长豇豆幼苗老茎叶和最嫩完全展开叶ABA含量升高,平均升幅香港青二芦白芦花白,但缺磷后期ABA含量二芦白香港青芦花白;缺磷胁迫下幼苗嫩茎叶ABA含量各品种都升高,升幅芦花白二芦白香港青。缺磷胁迫下长豇豆幼苗茎叶ABA含量的增加可能促进磷和水分的运输、减轻膜质过氧化,是对缺磷胁迫的适应机制之一。     

一种芦笋茎枯病防治喷头的试验研究

为促进本地区芦笋种植业规模化发展,提高芦笋种植产量,减少芦笋茎枯病防治用工成本,提出一种喷雾器喷头。采用该喷头进行施药,可以代替人工涂抹施药方式。对该喷头进行了试验研究,研究结果显示,3喷头喷雾器能明显降低芦笋茎枯病防治用工投入,且具有一定的茎枯病防治能力,在一定程度上可以替代人工涂茎作业,为芦笋规模化种植提供了基础。     

有机肥对长豇豆连作土壤养分及酶活性的影响

以连作3年的长豇豆土壤为对象,研究了不同来源的有机肥料对长豇豆连作土壤有机质、速效养分含量及土壤酶活性的影响。结果表明,在收获后施用鸡粪、猪粪为主的有机肥的土壤有机质含量、土壤有效N、P、K含量显著高于施用海产品残渣有机肥的;施用化肥的土壤有效N、P、K含量显著高于施用有机肥的。不同有机肥处理均提高了土壤pH值,而硫酸钾型复合肥处理使土壤pH值降低。土壤酶活性分析结果表明,在长豇豆的各生育期内,不同肥源有机肥处理均提高了土壤过氧化氢酶、脲酶、多酚氧化酶和蔗糖酶的活性,而化肥处理降低了土壤酶活性。在长豇豆采摘盛期,土壤酶活性最高,各处理间差异显著,其中,鸡粪处理效果最好,猪粪处理次之。     

芦笋花药培养胚状体诱导条件优化的初探

为建立一种效率较高的芦笋花药培养诱导胚状体的机制,实验以TH IELIM F1为试材,利用正交实验设计L16(45)探讨以MS为基本培养基附加NAA、6-BA、2,4-D、蔗糖、谷氨酰胺等五种因素对芦笋花药培养诱导胚状体的影响。结果表明:NAA是影响芦笋花药培养中诱导胚状体的主要因素,其次是6-BA、蔗糖,而2,4-D、谷氨酰胺的影响较弱。芦笋花药培养诱导胚状体的最优组合是0.01 mg/L NAA、2.0 mg/L 6-BA、1.0 mg/L 2,4-D、50 g/L蔗糖、0 mg/L谷氨酰胺。     

RAPD and SCAR markers linked to the sex expression locus M in asparagus

Bulk segregant analysis (BSA), random amplified polymorphic DNA (RAPD) and sequence characterized amplified region (SCAR) methods were used to map molecular markers to the sex locus M of asparagus. Two parents, A19 (male, Mm) and MW25 (female, mm), and 63 progeny were used for the study. Two DNA bulks, one male and one female, were made by pooling equal amounts of DNA from 10 randomly selected progeny of each sex type. A total of 760 arbitrary decamer oligonucleotide primers were used for RAPD analysis. Primer OPC15 produced two RAPD markers, OPC15-98 and OPC15-30, both of which were linked to the M locus at a distance of 1.6 cM. Subsequently, amplified RAPD fragment OPC15-98 was cloned and sequenced. The sequence was then used to design flanking 24-mer oligonucleotide SCAR primers SCC15-1 and SCC15-2. Both of these SCAR primers amplified a single 980 bp fragment; the same size as the cloned RAPD fragment. However, the SCAR marker was dominant as was the original OPC15-98 band from which it was derived. These RAPD and SCAR markers could be used for scoring male and female progeny in the mapping population, but were not found to be applicable to other asparagus germplasm studied. This revised version was published online in July 2006 with corrections to the Cover Date.