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1.
Ultrasound-assisted water extraction was optimized to recover gelling biopolymers and antioxidant compounds from Mastocarpus stellatus. A set of experiments following a Box–Behnken design was proposed to study the influence of extraction time, solid liquid ratio, and ultrasound amplitude on the yield, sulfate content, and thermo-rheological properties (viscoelasticity and gelling temperature) of the carrageenan fraction, as well as the composition (protein and phenolic content) and antiradical capacity of the soluble extracts. Operating at 80 °C and 80 kHz, the models predicted a compromise optimum extraction conditions at ~35 min, solid liquid ratio of ~2 g/100 g, and ultrasound amplitude of ~79%. Under these conditions, 40.3% carrageenan yield was attained and this product presented 46% sulfate and good mechanical properties, a viscoelastic modulus of 741.4 Pa, with the lowest gelling temperatures of 39.4 °C. The carrageenans also exhibited promising antiproliferative properties on selected human cancer cellular lines, A-549, A-2780, HeLa 229, and HT-29 with EC50 under 51.9 μg/mL. The dried soluble extract contained 20.4 mg protein/g, 11.3 mg gallic acid eq/g, and the antiradical potency was equivalent to 59 mg Trolox/g.  相似文献   
2.
张旭  李静  徐静 《热带作物学报》2017,38(5):849-853
植物抗氧化研究对寻找高效率、低毒性天然抗氧化剂具有重要的指导意义。以在海南分布较广的红树林植物海莲进行抗氧化活性研究,以期对海莲开发利用的可能性提供理论依据。采用清除DPPH自由基和清除ABTS自由基对海莲不同部位———根、茎、叶、花的乙酸乙酯提取物进行抗氧化能力评价,采用比色法和福林酚法测定总黄酮和总酚含量。结果显示,海莲茎的提取物在4个部位中抗氧化活性最好,清除二者的IC50分别为(0.234±0.053)mg/m L和(0.196±0.080)mg/m L,海莲中酚类物质含量较大,4个部位中茎的总酚含量最多,含量为13.2 mg。说明海莲各部位均显示出了较好的抗氧化能力,具有较好的开发潜力。  相似文献   
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Two-level full factorial design was employed to identify the extraction parameters that can improve the derivation of fucoxanthin content (FC), total carotenoid content (TCC), and antioxidant from two brown seaweeds, Sargassum siliquosum (SS) and Sargassum polycystum (SP). These parameters included temperature (A: 4–45°C), time (B: 30–1,440 min), and solvent-to-solid ratio (C: 10–50 ml/g). Antioxidant activities were determined as trolox equivalent antioxidant capacity (TEAC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity, and ferric-reducing antioxidant power (FRAP). Results showed that all three factors were significant (p < 0.05) in providing higher FC in both species. These factors were also significant in obtaining higher TCC in SS; whereas in SP, TCC was only affected by solvent-to-solid ratio. Only temperature was found to contribute significantly to a higher TEAC in both species. However, none of the factors improved DPPH for SS, except temperature and time for SP. For SS, only time was significant in obtaining higher FRAP; whereas temperature and time were significant for SP. Hence, results indicate that a simple modification in the extraction temperature, time, and solvent-to-solid ratio will be able to improve the derivation of fucoxanthin, carotenoids, and antioxidant activities.  相似文献   
4.
为减少亚硝酸盐在中式香肠中的用量,以茶多酚、壳聚糖、生育酚复配复合抗氧化剂,以中式香肠储藏期间的pH、TBA值、酸价和过氧化值为考察指标,采用正交试验优化低硝中式香肠中复合抗氧化剂的配方。结果表明,复合抗氧化剂可以部分取代亚硝酸盐,其最优配方为:茶多酚添加量0.10 g/kg,壳聚糖添加量1 g/kg,生育酚添加量0.03 g/kg,亚硝酸钠添加量0.04 g/kg;该配方下制得的低硝中式香肠的抗氧化效果明显优于单一使用亚硝酸钠的对照组。  相似文献   
5.
为探究油菜素甾醇对萝卜芽菜生物活性物质的影响,用表油菜素内酯及其类似物高芸薹素单独或者与氯化钠共同处理萝卜芽菜,测定并分析其芥子油苷、花青素、维生素C和总多酚的含量以及抗氧化能力的变化。结果表明,20 nmol·L~(-1)表油菜素内酯单独处理时,芥子油苷含量显著降低,但与40mmol·L~(-1)氯化钠共处理时,芥子油苷含量显著上升;20 nmol·L~(-1)高芸薹素单独处理或者与40 mmol·L~(-1)氯化钠共处理则对芥子油苷含量无显著影响。此外,表油菜素内酯和高芸薹素单独处理或者分别与氯化钠共处理均能显著提高花青素和维生素C的含量,但对总多酚含量和抗氧化能力均无显著影响。综上,表油菜素内酯与氯化钠共处理是提高萝卜芽菜中芥子油苷、花青素以及维生素C等主要生物活性物质的有效手段,这为通过化学调控的方法改良萝卜芽菜的品质提供了理论依据和技术支撑。  相似文献   
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Jamun (Syzygium cumini) is a tropical, underutilized fruit which is highly perishable in nature. It is a good source of vitamin C, tannins, gallic acid and anthocyanins and its beneficial effects are mostly due to the presence of bioactive compounds (pigments and phenolic compounds) in it. Due to astringent and fibrous nature, preparation of jam from jamun pulp is quite difficult, but other fruits (apple and kiwifruit) can be incorporated in it to improve its quality. This study aims to develop jam from blends of jamun with other fruits and analyse various physico-chemical, nutritional, textural and sensory properties. It was found that physico-chemical properties of jams were not found to vary greatly, but the jamun–kiwifruit jam was found to have fairly high amount of antioxidants(46.75 ± 0.67%), tartaric acid (26.24 ± 0.02 mg/100g sample), ascorbic acid (0.08 ± 0.01 mg/100 g sample) and lactic acid (23.95 ± 0.01 mg/100g sample) and lowest amount of 5-hydroxymethyl-2-furaldehyde (0.38 ± 0.04 mg/100 g sample). Jamun jam and jamun–kiwifruit jam possessed the texture required for jam while jamun–apple jam was found to be a relatively harder gel. Jam made with jamun and kiwifruit pulp was found to have highest acceptability on the basis of sensory evaluation.  相似文献   
9.
Increasing atmospheric carbon dioxide concentration (CO2) is an important component of global climate change that will have a significant impact on the productivity of crop plants. In recent years, growth and yield of agricultural crop plants have been shown to increase with elevated CO2 (EC) and have enticed considerable interest due to variation in the response of crop plants. In this study, comparative response of two mung bean cultivars (HUM‐2 and HUM‐6) was evaluated against EC at different growth stages under near‐natural conditions for two consecutive years. The plants were grown in ambient as well as EC (700 ppm) in specially designed open‐top chambers. Under elevated CO2, marked down‐regulation of reactive oxygen species (ROS) levels, membrane disruption and activities of superoxide dismutase and catalase were noticed in both the cultivars, but the extent of reduction was more in HUM‐6. As compared to ambient CO2, EC increased total chlorophyll, photosynthetic rate, growth and yield parameters. Cultivar‐specific response was noticed as HUM‐6 showed higher increase in yield attributes than HUM‐2. Under CO2 treatment, soluble protein and reducing sugars decreased while total soluble sugars and starch showed an opposite trend. Principal component analysis showed that both the cultivars responded more or less similarly to EC in their respective groupings of physiological and growth parameters, but the magnitude of ROS and antioxidative enzymes was variable. The experimental findings depict that both the cultivars of mung bean showed contrasting response against EC and paved the way for selecting the suitable cultivar having higher productivity in a future high‐CO2 environment.  相似文献   
10.
The aim of the present study was to investigate the long-term effect of using bee venom (BV) on the reproductive performance, immune, and health status of rabbit does and its effect on their litters. Sixty mature does, from Spanish V-line rabbit stock, were randomly assigned to four homogeneous groups with 15 does each. The 1st, 2nd and 3rd groups were injected twice weekly under the neck skin with 0.1 ml solution contains 0.1, 0.2 and 0.3 mg BV/rabbit respectively. The 4th group served as a control group. From the results, litter size at birth, litter weight and survival rate at weaning age as well as milk yield were significantly (≤ 0.05) increased in BV groups than in the control group. Serum estradiol 17-β (E2) was significantly (≤ 0.05) higher (15%) in the rabbit does treated with BV compared to the control group. The treated does with BV at any study doses showed a gradual and significant (≤ 0.05) decrease (12%) in serum progesterone levels (P4) compared to the control. They also showed a significant (p≤0.05) increase in conception (17%) and fertility rates (10%) compared to the control does. Treatment of rabbit does with BV caused a gradual and significant (≤ 0.05) reduction in both aspartate aminotransferase (AST) (16%) and alanine aminotransferase (ALT) (37%) liver enzyme activities. Additionally, results have shown that BV resulted in a gradual and significant (≤ 0.05) increase in total antioxidant capacity (TAC), antioxidative enzymes such as glutathione S-transferase (GST) and glutathione peroxidase (GPx), serum IgG, IgM and IgA levels with significant (≤ 0.05) decrease in malondialdehyde (MDA) and thiobarbituric acid reactive substances (TBARS) in BV groups compared to the control group. Results suggest that BV can be used in rabbit farming as an effective and safe alternative to artificial chemical drugs (sexual-stimulants) to improve certain reproductive traits, immune response and health.  相似文献   
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