转MsLEA4-4基因拟南芥株系的耐盐性分析

为了深入研究紫花苜蓿（Medicago sativa）MsLEA4-4基因的抗逆性，本试验对前期获得的转MsLEA4-4基因的拟南芥（Arabidopsis thaliana）株系进行不同浓度盐胁迫处理，以验证MsLEA4-4基因在拟南芥植株体内的耐盐性。试验结果表明：盐胁迫后转基因株系的发芽率达到55%，比对照植株（Control plant，CK）高81%；转基因株系的鲜重、叶绿素含量均高于CK（P<0.05）；盐胁迫后转基因株系的存活率比CK提高75.0%~81.3%；盐胁迫明显抑制根系生长，但转基因植株的侧根数量比CK多4~5根（P<0.05）；盐胁迫后转基因株系体内的可溶性糖含量（P<0.05）和超氧化物歧化酶（P<0.01），过氧化氢酶（P<0.01），过氧化物酶（P<0.05）活性均高于CK，脯氨酸（P<0.01）和丙二醛（P<0.05）含量均低于CK。综上可得，MsLEA4-4基因参与了拟南芥体内的耐盐性调控，提高了转基因拟南芥对盐胁迫的耐受性。本研究为进一步探索MsLEA4-4在转基因紫花苜蓿盐胁迫中的功能及培育耐盐性紫花苜蓿品种提供依据。     

Markov积分半群的逼近

通过证明Markov积分Q半群的逼近证明了l∞上一个Markov积分半群G(t)可以由一族一致收敛的积分半群逼近.     

渭北旱塬丰水年春玉米产量潜势及水肥效应研究

采用试验方法对谓北塬区春玉米进行旱作产量潜势和水肥效应定量研究，结果表明：该区丰水年份春玉米产量潜势为６０３．６Ｋｇ／亩，产量潜势系数为１．０，自然降水可完全满足春玉米生长需要。在此基础上所进行的人工田间供水（以提高土壤水分水平）会导致水分利用率的降侈低，但不会造成减产。相反，无论在何种水分条件下培肥地力，不仅可以大幅度提高春玉米产量，还能明显提高水分利用率。     

Characterization of Amaranthus cruentus L. seed proteins by 2-DE and LC/MS–MS: Identification and cloning of a novel late embryogenesis-abundant protein

Amaranth is taking great attention as an important cereal crop that could fulfill food requirements for the growing population, especially in developing countries. However, the protein composition of these seeds is not well known yet. We have used the proteomics tools to characterize amaranth seed proteome. About 400 proteins spots were resolved on two-dimensional gel electrophoresis (2-DE) and protein spots were analyzed by LC-MS/MS (liquid chromatography tandem mass spectrometry). Identified proteins were related to stress and defense responses, metabolic, respiratory, and oxide-reduction processes. One abundant spot was identified as a Late Embryogenesis Abundant (LEA) protein and the gene was cloned and characterized. The AcLEA cDNA contains a 418 bp open reading frame (ORF) encoding a polypeptide of 139 amino acids. Bioinformatics analysis showed that AcLEA belongs to LEAs Group 5. Proteomics is a powerful technique that could be used even in non-sequenced organisms such as amaranth. The obtained information reveals that amaranth seed, beyond the classical seed storage proteins, contains proteins related to protection against stress. The identification of these proteins opens the door to the application of new strategies to improve the quality of amaranth production.     

对合半群中元素的*·Moore-Penrose逆

本文给出了对合半群中元素的*·Moore-Penrose逆的十种刻划,证得了此广义逆的八个表达式,从而将〔2～5〕的相应结果推广到半群中。     

Analysis of regulation site and manner of abundant nodulation in a soybean (Glycine max (L.) Merr.) cultivar,kitantusunte, using grafting techniques

To characterize the regulation site and manner of the abundant nodulation in the soybean (Glycine max (L.) Merr.) cv. Kitamusume, three grafting eperiments were carried out as follows: reciprocal wedge grafting and inter-cultivar approach grafting between Kitamusume and a normal nodulating cultivar, Toyosuzu, as well as wedge grafting of scions of the supernodulating mutant En6500 onto either Kitamusume or Toyosuzu rootstock. In the reciprocal wedge grafting, the number of nodules per shoot dry weight and average weight per nodule in the grafted plants were consistent with those exhibited by the genotype of their rootstocks. Approach grafting did not affect the number of nodules per shoot dry weight on either side of the inter-cultivar approachgrafted plant. Although grafting of the mutant scion resulted in the loss of the autoregulatory response from the roots of both cultivars, difference in the number of nodules per g shoot dry weight still remained between the two cultivars. These results suggested that the abundant nodulation in Kitamusume is controlled by the root in a non-systemic manner and is independent of the autoregulation mechanism.     

Sequence analysis and expression pattern of AmLEA14 encoding a late embryogenesis abundant protein in Ammopiptanthus mongolicus

从沙冬青叶片EST文库中获得了胚胎晚期发生丰富蛋白（LEA）基因cDNA全长序列,命名为AmLEA14。序列分析表明该基因全长579bp,含有一个459bp编码152个氨基酸的开放阅读框,推测编码的蛋白质分子质量为16.6ku。二级结构预测显示此编码蛋白属于第4类LEA蛋白。系统发生分析表明,此编码蛋白与大豆LEA14蛋白（P46519.1）的亲缘关系最近。荧光定量PCR结果显示,AmLEA14的表达量在低温、干旱、盐胁迫条件下升高,但是主要在低温胁迫后期富集。推测AmLEA14基因可能在沙冬青抵御低温、干旱、盐胁迫中发挥作用,主要是参与沙冬青的低温防御机制。     

逆半直积的推广

主要研究了逆半群的半直积及其推广.首先给出了逆半群的半直积,定义了一种新的积——双半直积(它由集合M={(a,s)∈S×T(a,s)(b,t)=(ab,sbta)}构成,其中sb=sβ(b),ta=ta(a)).然后讨论了半格与纯正半群的双半直积的性质.最后证明了半格与纯正半群的双半直积的是一个纯正半群.     

几何突变的人口半群的单调性和FRR性质

研究几何突变人口半群的单调性和FRR性质.证明了q-矩阵Q在l∞生成正的压缩半群;在c0空间上生成连续压缩半群;最小Q-函数P（t）是FRR的,并且给出了其为随机单调的判别标准.     

一类时滞系统的解半群的范数连续性

讨论了时滞线性系统的解半群的范数连续性,其方法是:在一个特定的相空间中,把时滞系统转化为无时滞的抽象柯西问题,再运用半群理论解决解半群的范数连续性.