黄政德教授应用四妙丸的临床经验举隅

四妙丸是中医经典方剂，组方立意独特，常用于治疗湿热下注、两足麻木、痿软、肿痛诸证。黄政德教授结合自己的临床经验，审证求因，灵活应用四妙丸随证加减治疗痛风性关节炎、慢性宫颈炎、弱精症及脑梗死后遗症等各种疾病，临床疗效显著，体现了中医"辨证论治""异病同治"的思维。     

Role of Akt signal pathway in inhibition of neuronal apoptosis by Chinese medicine Zuogui pill in adult rats treated with monosodium glutamate

AIM: To explore the role of Akt signal pathway in apoptosis of neural cells in adult rats treated with Zuogui Pill, a Chinese medicine. METHODS: Flowcytometry and Western blotting methods were used to investigate the changes of cellular apoptosis rate and Akt signal pathway. RESULTS: Monosodium glutamate (MSG) could increase cellular apoptosis rate and significantly restrained the phosphorylation of Akt (Ser473) and Akt (Thr308), and markedly increased the levels of phospho-FKHR (ser256), GSK-3β (Ser9) and PTEN. Zuogui Pill partly inhibited the above effects of MSG. CONCLUSION: Zuogui pill effectively inhibits the neural apoptosis induced by MSG, and Akt pathway is involved in the neuronal protection of Zuogui pill.     

Effects of blocking the expression of PKARⅠβ gene with antisense nucleotide on Shuang long-Jie gu Pill (SLJGP) containing serum osteoblast proliferation

AIM: To further investigate the role of PKARⅠβ in the growth-promoting effects of shuang long Jiegu pill (SLJGP), a Chinese medicine, on cultured osteoblasts. METHODS: pcDNA- antiPKARⅠβ, a recombinant expressing the antisense sequence of PKARⅠβ, was constructed and transformed HFOB1.19 by lipofectin. MTT was undertaken to assess the cell growth with the treatment of high dosage of SLJGP containing serum. RESULTS: Antisense gene blocked the growth-promoting effects of SLJGP containing serum on HFOB1.19. CONCLUSION: The function of SLJGP is closely related to cAMP-dependent protein kinase A.     

Evaluation of fabric pilling using hybrid imaging methods

A study has been made on the quantification and evaluation of fabric pilling using two-dimensional and three-dimensional hybrid imaging methods. Two-dimensional imaging method was good for some samples while three-dimensional measurement method for others, according to the properties of their base fabric. Various image processing techniques as well as three-dimensional data processing algorithms were applied for the extraction of pills from measured data and a series of shape parameters have been defined for the objective evaluation of fabric pilling. An evaluation criterion that is compatible with the conventional evaluation method has been proposed by applying the new evaluation method to the current photographic standards.     

HPLC同时测定栀子金花丸中4种成分的含量

[目的]采用HPLC建立同时测定栀子金花丸中黄芩苷、汉黄芩苷、黄芩素、汉黄芩素这4种成分含量的分析方法。[方法]色谱柱为Agilent Zorbax SB-C18(4.6 mm×250 mm,5μm);流动相为乙腈-浓度0.1%磷酸溶液,梯度洗脱,检测波长为278 nm。[结果]黄芩苷、汉黄芩苷、黄芩素、汉黄芩素4种成分的线性范围分别为0.418~4.18μg/ml(R=0.999 8),0.2~2.0μg/ml(R=0.999 8),0.233~2.330μg/ml(R=0.999 9),0.215~2.150μg/ml(R=0.999 9);平均回收率分别是98.40%(RSD=0.45%)、98..66%(RSD=1.41%)、98.88%(RSD=1.48%)和98.97%(RSD=1.45%)。[结论]该方法快速、简便,且具有良好的重复性和回收率,可作为栀子金花丸中这4种成分的含量控制方法。     

补肾、活血、祛痰法对激素性股骨头坏死模型血液流变学的影响

目的 观察补肾、活血、祛痰三种中医治法对激素性股骨头坏死模型兔血液流变学的影响。方法 在运用改良Matsui方法成功制备兔激素性股骨头坏死模型后，108只白兔随机分为正常对照组、模型对照组、通络生骨组、补肾组、活血组、祛痰组共6组，每组18只，通络生骨组、补肾组、活血组、祛痰组分别给予通络生骨胶囊、右归饮、桃红四物汤和苓桂术甘汤灌胃，其余各组则灌服同容量生理盐水。在实验第2周，第4周，第8周分别抽血检查血液流变学指标。结果 与正常对照组比较，模型对照组第2、4、8周血液流变学指标均维持在较高水平（P<0.01）。在第2周时，通络生骨组、活血组、祛痰组与模型对照组比较，均得到下降（P<0.05），其中又以通络生骨组、活血组改善更明显，该两组在实验第8周时恢复至正常白兔水平（P>0.05）。补肾组在实验第2周时较模型对照组改善不明显（P>0.05），至第4周时才有明显改善（P<0.05）。结论 桃红四物汤较右归饮、苓桂术甘汤能迅速改善激素性股骨头坏死血液流变学指标，右归饮、苓桂术甘汤对血液流变学指标的改善作用可能与其它途径相关，提示补肾、活血、祛痰三种中医治法治疗激素性股骨头坏死的作用机制可能不同。     

Effects of Zuogui Pill (左归丸) on Wnt Singal Transduction in Rats with Glucocorticoid-induced Osteoporosis

Objective:To reveal the mechanism of Zuogui Pill (左归丸) in treatment of glucocorticoid-induced osteoporosis from the angle of the Wnt signal transduction pathway and to provide further experimental e...     

通脉滴丸的成型工艺研究

为研究通脉滴丸的最佳成型工艺,以丸重变异系数、溶散时限及外观质量为评价指标,以基质种类、药物与基质的配比、滴制温度及冷却液温度为考察因素进行正交试验。结果表明:优选的最佳成型工艺为药物与基质配比为1∶1.5、基质为PEG6000、滴制温度为70~80℃、冷却液温度为10~15℃。优选的成型工艺简便易行,成品得率高,且有良好的重现性和可操作性。