不同规格野生黄鳍金枪鱼肌肉营养分析与评价

探究不同规格黄鳍金枪鱼(Thunnus albacares)肌肉营养成分及品质差异,实验以野捕的 3种规格黄鳍金枪鱼J1 (4.2±1.2) kg、J2 (22.5±2.5) kg和J3 (50.8±3.9) kg为研究对象,通过常规生化分析方法对金枪鱼肌肉的常规营养成分、氨基酸、脂肪酸及矿物质元素进行比较分析。结果显示,(1) J1组水分含量显著高于J2、J3组;J2、J3组粗蛋白含量显著高于J1组(P<0.05);J3组粗脂肪含量显著高于J1、J2组(P<0.05)。(2)检出19种常见氨基酸,氨基酸含量最高的为谷氨酸(3.04~ 3.25 g/100 g),必需氨基酸中含量最高的为赖氨酸(2.02~2.15 g/100 g),最低的为色氨酸(0.31~ 0.45 g/100 g)。非必需氨基酸含量J3>J2>J1 (P<0.05);必需氨基酸、呈味氨基酸含量J1组显著低于J3组(P<0.05)。依据氨基酸评分(AAS),缬氨酸为第一限制性氨基酸;以化学评分(CS)为评分标准,J1、J2组第一限制性氨基酸为色氨酸,J3组为苯丙氨酸+酪氨酸。(3)各组共检出25种脂肪酸,以多不饱和脂肪酸(PUFA)为主,含量最高的为二十二碳六烯酸(DHA),占总脂肪酸含量的37.46%~39.18%。DHA含量J3组显著高于J1、J2组;二十碳五烯酸(EPA)含量J2、J3组显著高于J1组;DHA∶EPA比值J1组显著高于J2、J3组(P<0.05)。单不饱和脂肪酸(MUFA)含量J3>J2>J1;PUFA含量J3组显著高于J1、J2组(P<0.05)。PUFA/饱和脂肪酸(SFA)、n-3系多不饱和脂肪酸/n-6系多不饱和脂肪酸(n-3/n-6)比值J2、J3组显著高于J1组(P<0.05)。h/H比值J3组显著高于J1、J2组(P<0.05)。(4) J2、J3组Na、Ca含量显著高于J1组,J1组K含量最高且显著高于J2、J3组(P<0.05)。4种重金属元素均低于食品中建议的最大允许限量,其中Fe含量最大的为J3组,且J3>J2>J1 (P<0.05);Cu含量最大的为J3组,且显著高于J1组(P<0.05)。综合分析,大规格黄鳍金枪鱼具有更好的营养质量,本研究为居民膳食的选择及黄鳍金枪鱼人工配合饲料的配制提供了科学依据。     

Molecular evidence for cosmopolitan distribution of platyhelminth parasites of tunas (Thunnus spp.)

Global distribution of platyhelminth parasites and their host specificities are not well known. Our hypothesis was that platyhelminth parasites of large pelagic fishes are common around the world. We analysed molecular variation in three different taxa of platyhelminth parasites infecting four species of tunas: yellowfin tuna (Thunnus albacares, Scombridae) from Western Australia, southern bluefin tuna (Thunnus maccoyii, Scombridae) from South Australia, Pacific bluefin tuna (Thunnus orientalis, Scombridae) from Pacific Mexico and northern bluefin tuna (T. thynnus, Scombridae) from two localities in the Mediterranean (Spain and Croatia). Comparisons of ITS2 and partial 28S rDNA demonstrated two congeneric species of blood flukes (Digenea: Sanguinicolidae) from multiple hosts and localities: Cardicola forsteri from southern bluefin and northern bluefin tunas, and Cardicola sp. from Pacific bluefin and northern bluefin tunas; and a gill fluke, Hexostoma thynni (Polyopisthocotylea: Hexostomatidae), from yellowfin, southern bluefin and northern bluefin tunas. Partial 28S rDNA indicates that a second type of fluke on the gills, Capsala sp. (Monopisthocotylea: Capsalidae), occurs on both southern bluefin and Pacific bluefin tunas. This appears to be the first report of conspecific platyhelminth parasites of teleosts with a wide‐ranging geographical distribution that has been confirmed through molecular approaches. Given the brevity of the free‐living larval stage of both taxa of flukes on the gills (H. thynni and Capsala sp.), we conclude that the only feasible hypothesis for the cosmopolitan distribution of these flatworms is migrations of host tunas. Host migration also seems likely to be responsible for the widespread occurrence of the two species of blood flukes (Cardicola spp.), although it is also possible that these were translocated recently by the spread of infected intermediate hosts.     

基于分位数回归的大西洋中部公海大眼金枪鱼(Thunnus obesus)栖息环境综合指数

摘要：2001年7月4日-10月27日，中国水产总公司所属的3艘大洋性超低温金枪鱼延绳钓渔船对大西洋中部公海海域进行金枪鱼渔业调查。本文根据所获得的渔场环境、作业参数、渔获统计等数据，应用栖息地适应性指数（habitat suitability index, HSI）模型并结合分位数回归分析方法分别对有关水层（60m为一层）及总渔获率与温度、盐度和相对流速等环境因素的关系并考虑其不同的影响权重及交互作用建立了数值模型，根据该模型计算大眼金枪鱼的栖息环境综合指数(HEII)，利用MarineExplore4.0绘制大眼金枪鱼HEII指数等值线分布图。结果显示：（1）180～240m水层中，中部海区的 指数分布较高（高于0.6），其他海区分布较低（小于0.6）；（2）240～300m水层中，6ºN以南的海区 分布较高（高于0.6），以北的海区分布较低；（3）300～360m水层中，仅南部部分海区（4ºS，23ºW附近） 分布较高，其他大部分海区分布很低（低于0.3）；（4）7ºN以南的大部分海区 指数分布较高（高于0.6），仅在部分较小海区（5ºN，22ºW、2ºS，17ºW和2ºS，23ºW的附近）内 指数分布低于0.5。 关键词：大眼金枪鱼；栖息环境综合指数；分位数回归；大西洋中部公海     

Bigeye tuna (Thunnus obesus) vertical movements in the Azores Islands determined with pop-up satellite archival tags

Movement patterns of 17 bigeye tuna (Thunnus obesus) near the Azores Islands were analyzed between April and May 2001 and 2002 using pop‐up satellite archival tags. Despite short attachment durations (1 to 21 days, 8.2 days on average), their vertical movements revealed much shallower distribution of bigeye tuna in comparison with previous studies in the tropical Pacific and tropical Atlantic. Depth and temperature histograms were unimodal, although overall depth distribution during the day was deeper than during the night due to daily incursions in deeper waters. Although generalized additive models showed significant non‐linear relationships with weight of the fish and sea level anomaly (as a proxy for variability of thermocline depth), the effect of these variables on bigeye depth appeared minor, suggesting that vertical movements of bigeye in the Azores during the spring migration may be influenced by food availability in upper water layers.     

南太平洋延绳钓长鳍金枪鱼生物学组成及其与栖息环境关系

长鳍金枪鱼(Thunnus alalunga)经济价值高,是我国延绳钓渔业重要的目标鱼种。根据2013年9月~2014年1月和2014年4~8月我国金枪鱼观察员在南太平洋东部海域收集的长鳍金枪鱼样本和海洋环境数据,对其生物学组成和栖息环境进行了研究。结果表明:叉长(FL,cm)与体质量(WW,kg)的关系为:WW=3×10-5×FL2.909 9(雌雄性,R2=0.915 3);体长(TL,cm)与叉长(FL,cm)关系为:TL=1.033 6FL+2.555(R2=0.961 4);叉长(FL,cm)与两背鳍间距(LD1D2,cm)的关系为:LD1D2=0.248 5FL+1.238 1(R2=0.815 1);利用各水层长鳍金枪鱼渔获率(catch per unit effort,CPUE)推测其主要的栖息水层为150~270 m,栖息水层温度范围16~22℃,盐度范围35.0~35.6,其中最高资源丰度主要分布在190~230 m的水层,对应的温度为18~20℃,盐度为35.2~35.4。研究结果可为掌握南太平洋长鳍金枪鱼栖息环境提供基础数据。     

印度洋中南部大眼金枪鱼生物学特性研究

根据2008年9月~2009年4月在印度洋中南部金枪鱼延绳钓渔场调查期间收集的大眼金枪鱼生物学数据,对其基本的生物学特征进行了研究。结果表明:(1)叉长范围为57~184 cm,优势叉长组为101~110cm和121~130 cm,约占总数的41.7%,平均叉长为119.5 cm;(2)不分性别大眼金枪鱼叉长(FL)与加工后重(DW)的关系可表达为:DW=2.407 6×10-5FL2.931 6,雌雄个体差异不显著(F=0.207,P0.05);(3)调查期间,大眼金枪鱼雄性比例为56.55%,当叉长大于140 cm时,雄性占优势;调查期间,该海域大眼金枪鱼的繁殖期为10月~翌年4月,繁殖峰期为10月~翌年1月;(4)摄食种类中柔鱼、帆蜥鱼和虾类所占比重较高,分别占36.0%、20.1%和18.0%;各月份摄食等级1~4级分布呈显著性差异(χ2=191.20,P0.01),各叉长组大眼金枪鱼摄食等级变化无显著性差异(χ2=41.08,P0.05)。     

Feeding habits of albacore Thunnus alalunga in the transition region of the central North Pacific

ABSTRACT:   The feeding habits of albacore Thunnus alalunga (fork length: 48.9–76.2 cm, n  = 132) were examined from late spring to early autumn in relation to its northward migration in the transition region between the subtropical and subarctic fronts in the central North Pacific. Samples were collected at night using surface gill nets or during daytime pole-and-line surveys in 2001 and 2002. During May and June, albacore fed mainly on Japanese anchovy Engraulis japonicus , which accounted for 27.2%, 67.0%, and 45.5% of the total stomach contents by number ( Cn ), wet weight ( WW ), and frequency of occurrence ( F ), respectively, and secondarily on the subarctic gonatid squid Gonatopsis borealis ( Cn , 15.8%; WW , 10.8%; F , 28.8%). From July to September, albacore continued to depend on Japanese anchovy ( Cn , 48.2–52.8%; WW , 79.9–95.2%; F , 27.8–85.4%). These results corresponded well with the remarkable rebound of the Japanese anchovy stock since the 1990s. Gonatopsis borealis , the main squid prey from May to June, almost disappeared from the stomachs of albacore from July to September, probably due to the northward migration of this squid to subarctic waters in summer. The feeding impact of albacore on the Japanese anchovy stock in the transition region was conservatively estimated to be from 1400 to 2100 tons per day from late spring to early autumn.     

应用栖息地指数对印度洋大眼金枪鱼分布模式研究

运用分位数回归方法对温度、温差、氧差与印度洋大眼金枪鱼延绳钓钓获率进行二次回归分析,找出最佳上界方程,以最佳上界方程拟合的数值来建立栖息地指数(HSI)模型,从而揭示印度洋大眼金枪鱼栖息地的分布模式。研究表明,温度、温差、氧差与印度洋大眼金枪鱼延绳钓钓获率的最佳上界分位数回归方程分别为HR_(T0.9)=-44.803 7.685T_(0.9)-0.255T_(0.9)~2,HR_(dT_(0.9))=6.234 0.953dT_(0.9)-0.026dT_(0.9)~2和HR_(dO_(0.88))=7.422 4.25dO_(0.88)-0.727dO_(0.88)~2。10°N～10°S间印度洋海域大眼金抢鱼HSI指数达到0.9以上;10°N以北的波斯湾及10°S～15°S海域的HSI指数为0.8～0.9;15°S～40°S之间海域HSI指数介于0.7～0.8,其中50°E～90°E、15°S～25°S间存在一片季节性HSI指数<0.7的区域;40°S以南的海域HSI指数<0.6。     

印度洋大眼金枪鱼、黄鳍金枪鱼延绳钓渔场重心变化分析

大眼金枪鱼和黄鳍金枪鱼是印度洋金枪鱼延绳钓的主要渔获种类.通过计算以渔获量为权重的经度和纬度的加权平均值即渔场重心和利用空间距离分析方法,分别对1967-1987年、1988-2004年两个时间段的印度洋大眼金枪鱼、黄鳍金枪鱼延绳钓渔场重心的年内、年际变化规律和各月渔场重心空间聚类情况进行研究.研究表明,渔场重心在年内呈现出规律性变化,大眼金枪鱼月重心变化1-4月向西北移动,5-8月向东南方向移动,9-12月向东北方向移动;黄鳍金枪鱼月渔场重心变化1月向西北移动,2-4月向西移动,5-8月份向东南移动,9-12月份向东北移动,这在一定程度上反映了印度洋大眼金枪鱼、黄鳍金枪鱼的洄游规律.渔场重心的年间变化受到了海洋环境条件变化所引起的南方涛动现象(ENSO)的影响,大部分发生El Nio的当年或次年,年渔获量分布重心会向南、向西移;发生La Nia的年份前后渔获量分布重心会向北、向东移.同时捕捞行为也对渔场重心的年间变化产生影响.     

所罗门群岛海域长鳍金枪鱼的垂直分布与环境因子的关系

根据2010年8-11月在中西太平洋所罗门群岛海域金枪鱼延绳钓作业,研究了长鳍金枪鱼的垂直分布与环境因子的关系。结果表明,在所罗门群岛海域,长鳍金枪鱼的最适水层为130．00～160．00m,最适水温范围为22．00～24．00℃．最适盐度范围为35．70～35．80;长鳍金枪鱼活动较频繁的水层范围为130．00-190．00m,水温范围为20．00-26．00℃,盐度段为35．60～35．90。