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1.
WANG Hong 《东北农业大学学报(英文版)》2009,16(2):83-90
This paper discussed the definition and essential factors of green agriculture, including green ecological environment, green material energy input, application and the popularization of green agricultural technology, the green behavior of worker, green policy regulations guarantee, fund support, supply and the production of green agriculture products, as well as the concept of green agriculture, structural mechanism and function. And finally existing problems in present green agriculture and five suggestions of green agricultural sustainable develonment were proposed. 相似文献
2.
分别从绿色农业科技信息网站、数据库、多媒体服务平台、科技咨询、研究机构和专业协会合作组织等几个方面深入研究了国外绿色农业科技信息资源建设情况及成功经验,提出了对北京市相关工作的对策建议。 相似文献
3.
浅议我国绿色旅游 总被引:8,自引:0,他引:8
张洁 《绿色中国(A版)》2005,(18):57-58
文章探讨了我国绿色旅游的兴起、内涵、特点和功能等,讨论了绿色旅游、生态旅游、自然旅游的区别与联系,分析了我国绿色旅游的发展现状和对策。 相似文献
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Field experiments were conducted at Tamil Nadu Rice Research Institute, Aduthurai, India, during the wet seasons of 1992 and 1993 to study the effect of full and partial substitution of fertiliser N with green manure N (Sesbania rostrata) on nitrogen uptake, yield attributes and yield of rice. The experiment consisted of eight treatments with two levels of N (100 and 200 kg ha−1 ) and three sources of N application viz., fertilizer, integrated (1:1 fertilizer and green manure N) and green manure N compared to the recommended practice (150 kg fertilizer plus 6.25 t ha−1 (72 kg N) green manure) and a no N control. Nitrogen application markedly increased the N uptake. Combined use of the two N sources at 200 and 222 kg N ha−1 and of single fertilizer N at 200 kg N ha−1 recorded the maximum N uptake, increased the yield attributes such as number of panicles per unit area, weight per panicle, number of total and filled grains per panicle and test weight. At 200 kg N ha−1 full substitution of N by green manure reduced the grain yield but only partial substitution of N by green manure resulted in almost similar yield as single fertilizer N. Thus 200 kg N ha−1 applied in equal proportions of fertilizer and green manure N can be recommended for medium duration rice cultivars. 相似文献
7.
单抗酶联试剂盒与PCR方法快速检测沙门氏菌的比较 总被引:18,自引:1,他引:17
本文对单抗酶联试剂盒与PCR方法快速检测沙门氏菌进行了比较。将沙门氏菌与大肠杆菌以不同比例混合后 ,分别用ELISA和PCR法检测 ,在 1∶1 0 0的比例时 ,用这 2种方法均能检测出阳性样品 ;在 1∶1 0 0 0的比例时 ,用ELISA法检测为阴性 ,而用PCR法则能检测出。检测鲜牛奶、龙虾、虾仁、熟食制品等样品 ,2种方法的符合率达 97 6 %。对冻虾仁、人粪便样品的前增菌、选择性增菌、后增菌过程进行同步检测 ,在样品的前增菌液 ,直接ELISA法检测的OD值小于 0 5 ,而PCR法扩增能出现特异条带 ,经国标方法验证为阳性 ,证实PCR法的敏感性优于直接ELISA法 ,而在选择性增菌液和M 肉汤后增菌液中 ,2种方法均能检出阳性样品。 相似文献
8.
从海南省杂草胜红蓟和假马鞭上检测到粉虱传双生病毒 总被引:9,自引:0,他引:9
利用三抗体夹心ELISA(TAS-ELISA)及PCR检测的方法对采自海南的胜红蓟(Ageratum conyzoides)和假马鞭(Stachytarphetajamaicensis)的5个病样进行了检测,表明均为粉虱传双生病毒。PCR扩增产物克隆后进行序列测定,结果表明存在2类双生病毒,其中样品Hn2存在2类病毒的复合侵染。这是在海南首次报道存在有粉虱传双生病毒。 相似文献
9.
D. Boscia H. Zeramdini M. Cambra O. Potere M.T. Gorris A. Myrta B. Di Terlizzi V. Savino 《European journal of plant pathology / European Foundation for Plant Pathology》1997,103(5):477-480
A monoclonal antibody to an Albanian isolate of plum pox potyvirus (PPV) was obtained (MAbAL), that specifically recognized strain M of this virus. The specificity of MAbAL, assessed by comparative ELISA on 130 PPV isolates of different geographical origin, 22 of which were also tested by comparative IC-PCR, gave consistent and highly reproducible results. MAbAL seems to be elicited by a stable surface determinant that makes it particularly suitable for successful use under a wide range of conditions. MAbAL is an useful addition to the panel of PPV-specific MAbs available to date. 相似文献
10.
Nataa Mehle Maja Kova
Nataa Petrovi
Marua Pompe Novak pela Baebler Hana Kre
i
Stres Kristina Gruden Maja Ravnikar 《Physiological and Molecular Plant Pathology》2004,64(6):19
The aim of this work was to correlate the appearance of the symptoms, multiplication and spread of virus after mechanical inoculation of potato (Solanum tuberosum L.) cultivars showing different levels of susceptibility and sensitivity to Potato virus YNTN (PVYNTN). The potato cultivars used were the resistant cultivar Sante and susceptible cultivars Igor, Pentland squire and Désirée. The spread of the virus PVYNTN in infected plants was monitored using different methods: DAS-ELISA, tissue printing, immuno-serological electron microscopy and real-time PCR. In all three susceptible cultivars, the virus was detected in the inoculated leaves 4–5 days after inoculation. From there virus spread rapidly, first into the stem, then more or less simultaneously to the upper leaves and roots. Real-time PCR was shown to be very sensitive and enabled viral RNA to be detected in non-inoculated leaves of susceptible cultivar Igor earlier than other methods. Therefore, for exact studies of plant–virus interaction, a combination of methods which detect viruses on the basis of their different properties (coat protein, morphology or RNA) should be used to monitor the spread of viruses. 相似文献