淫羊藿—蜂胶佐剂对雏鸡免疫器官早期发育和形态结构的影响

３日龄雏鸡按两个剂量组分别于皮下注射淫羊藿－蜂胶佐剂（简称淫蜂佐剂）０．２ｍＬ和０．４ｍＬ，２４日龄时重复注射１次，于７，１４，２１，２８，３５，４２日龄时随机抽样扑杀，采取免疫器官称重并进行光镜和电镜检查，比较不同剂量的淫蜂佐剂对雏鸡免疫器官的增重以及形态结构的影响。结果表明，淫蜂佐剂能显著促进雏鸡免疫器官的早期发育，并能刺激Ｔ，Ｂ淋巴细胞活化。重复注射后，可再次引起脾脏增重，且与剂量有相关性，     

真理蜂胶营养液调节血脂作用的研究

真理蜂胶营养液是以蜂胶为主要原料，采用特殊工艺生产的一种具有蜂胶独特风味、酸甜适口的新型天然保健食品。本产品通过口给高血脂大鼠的实验，结果可显著抑制和降低ＴＧ和ＴＣ，升高ＨＤＬ－Ｃ水平，且高、中、低剂量组与对照组相比，均有显著性差异（Ｐ＜００５）。     

蜂花粉、蜂胶、蜂王浆、蜂蜜抗氧化作用的研究

[目的]比较蜂蜜、蜂王浆、蜂胶、蜂花粉的抗氧化效果,为其今后在抗氧化方面的应用提供理论依据,并指导人们正确地选择具有抗氧化活性的蜂产品。[方法]以蜂花粉、蜂胶、蜂王浆和蜂蜜为材料,采用Schaa烘箱法和羟自由基清除法测定4种蜂产品的抗氧化活性。[结果]4种蜂产品均具有较强的抗氧化作用。随着时间的延长,它们的POV值升高,在36 h内表现出较好的抑制油脂氧化的效应,其中蜂胶、蜂王浆和蜂花粉的抗氧化效果优于蜂蜜。在一定时间内,蜂花粉、蜂胶、蜂王浆、蜂蜜均能抑制大豆油的氧化,对羟基自由基的清除率分别为:65.93%、82.08%2、6.95%、27.20%。[结论]蜂花粉、蜂胶、蜂王浆和蜂蜜能够清除体内过多的自由基,降低过氧化脂质的生成,是很好的抗氧化产品。     

冻干蜂胶粉的研究

将提纯蜂胶与不同组分的赋型剂相配合,利用冷冻干燥技术制成了蜂胶冻干粉。该产品具有易加工、粉碎等特点,可广泛应用于医药、食品、日用化工、畜牧业等领域。     

蜂胶片对动物的急性毒性试验与遗传毒性试验的研究

本试验以康思农蜂胶片为材料对动物进行食品安全性毒理学试验，采用霍恩氏法对试验动物小鼠进行急性毒性试验，采用Ames法，对小鼠进行遗传毒性试验。试验结果表明，康思农蜂胶片属实际无毒级。未见任何遗传性毒性作用。     

蜂胶对NDV的灭活作用效果观察

本文通过鸡胚接种的方法就蜂胶乙醇浸出物对NDV的灭活作用进行了试验观察,结果表明常温下3分钟,含蜂胶乙醇浸出物0.1mg/ml的95%乙醇稀释液、含蜂胶乙醇浸出物10mg/ml的47.5%乙醇稀释液、含蜂胶乙醇浸出物10mg/ml的生理盐水稀释液;37℃3分钟,含蜂胶乙醇浸出物0.1mg/ml的95%乙醇稀释液、含蜂胶乙醇浸出物0.1mg/ml的47.5%乙醇稀释液、含蜂胶乙醇浸出物10mg/ml的生理盐水稀释液均对ND克隆-30具有完全灭活作用;常温下3分钟,1mg/ml的47.5%乙醇稀释液对ND克隆-30具有灭活作用。     

迪卡白种公猪肢蹄病的调查与蜂胶防治报告

本文对某工厂化养猪场的迪卡白种公猪40头进行了肢蹄病的观察、诊治和预防,结果:(1)在40头种公猪中发现32头患有不同程度的肢蹄病变(占80％)。在32头患猪中严重和有跛行的9头(占28.13％),前肢肢跡病17头(占53.13％),后肢肢蹄病29头(占90.63％);(2)所检出的肢蹄病按其发生部位和病变不同,共有26种之多。其中在蹄趾(指)部位的病变有蹄壁磨损、蹄踵腐损、蹄踵青肿、趾(指)间腐烂、趾(指)间增殖、蹄裂、假性蹄裂、趾(指)底腐损、蹄冠脓肿、白绒病等20种。在肢体关节部位的病变有腕关节皮肤磨损、感染化脓与关节炎、附关节皮肤磨损、化脓与关节炎等6种;(3)发病的主要因素是猪栏环境与地面的质地不良直接引起患部的磨损。继发感染的主要病原菌为坏死梭状杆菌和化脓棒状杆菌;(4)在防治方面采取以蜂胶为主的综合防治措施效果良好。     

Effect of propolis water extract on apoptosis of human umbilical vein endothelial cells in vitro

AIM: To explore the effect of water extract propolis (WEP) from Taishan on apoptosis of human umbilical vascular endothelial cells (HUVECs) induced in vitro by tumor necrosis factor-α (TNF-α). METHODS: HUVECs were collect by digestion-perfusion and cultivated. TNF-α at concentration of 50 μg/L was administrated to induce the apoptosis of HUVECs. After injury, HUVECs were treated with WEP at concentrations of 50, 100, and 200 mg/L, respectively, for 24 h. Apoptosis was evaluated by terminal-deoxynucleotidyl transferase mediated nick end labeling (TUNEL) and flow cytometry (FCM). RESULTS: Apoptosis index in injured group was significantly higher than that in control group, and decreased significantly after treating with WEP at concentrations of 50, 100, and 200 mg/L, respectively (P<0.01). CONCLUSION: WEP may be useful for protection of vascular endothelial cells by inhibiting apoptosis.     

Evaluation of propolis effects on some biochemical parameters in rats treated with sodium fluoride

The present study in which 42 female rats, each weighing 200−250 g, were used covered a period of 21 days. The animals were divided into six groups. The first group served as the control group, whereas Group 2 was administered propolis at a dose of 200 mg/kg/bw in drinking water for 21 days. Group 3 was first provided with normal drinking water for a period of 14 days, and was subsequently administered propolis at a dose of 200 mg/kg/bw in drinking water for 7 days. Group 4 was first given normal drinking water for 14 days, and was secondly administered 100 ppm fluoride as a sodium fluoride in drinking water for 7 days. Group 5 was first administered propolis alone at a dose of 200 mg/kg/bw in drinking water for 14 days, and was secondly administered 100 ppm fluoride in association with 200 mg/kg/bw propolis for 7 days. Finally, Group 6 was first provided with normal drinking water for 14 days, and was secondly administered 100 ppm fluoride in association 200 mg/kg/bw propolis for a period of 7 days. At the end of the 21st day, blood samples were collected from the heart of each animal into both heparinised tubes and tubes without anticoagulants. Glucose, triglyceride, cholesterol, total protein, and uric acid levels, and aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) activities in the serum, as well as malondialdehyde (MDA) levels, glutathione peroxidase (GSH-Px) in the plasma, erythrocyte superoxide dismutase (SOD) and catalase (CAT) activities were measured. When compared to the control group, statistical differences were determined to exist with respect to oxidative stress parameters which involved increase in MDA levels in Groups 4−6, decrease in SOD activity in Groups 4 and 6, increase in CAT activity in Groups 5 and 6, and decrease in GSH-Px activity in Groups 4 and 6. Furthermore, in comparison to the control group, significant differences were observed with respect to certain serum biochemical parameters, including decrease in glucose levels in Groups 5 and 6, decrease in triglyceride levels in Groups 2 and 4, decrease in cholesterol levels in Groups 2 and 5, decrease in the total protein level of Groups 4−6, decrease in the ALT activity of Groups 5 and 6, increase in the AST activity of Group 4, decrease in the ALP activity of Groups 2−6 and increase in the uric acid level of Group 2. In the groups that were administered propolis in association with fluoride, improvement was observed in some oxidative stress parameters and certain other biochemical parameters. Changes determined in the oxidative stress parameters (especially MDA and SOD) were indicative of the anti-radical activity of propolis on the free radicals generated by sodium fluoride. However, the values not drawing completely close to those of the control group can be explained with propolis not being able to completely eliminate the free radicals and the other adverse effects generated by fluoride.     

Effect of propolis on the expression of CD54 and activation of NF-κB p65 of lung tissue in acute lung injury rats

AIM: To study the effect of propolis on the expression of CD54 and activation of NF-κB p65 in lung tissue of acute lung injury (ALI) rats. METHODS: 40 male Wistar rats were divided into 5 groups: normal control, model control, dectancyl group, water soluble derivative of propolis (WSP) group and ethanol extracted propolis (EEP) group. ALI animal model was performed by oleic acid and LPS twice attack. The pathologic slice was observed with light microscope and the NF-κB p65 activity and CD54 expression were tested by immunohistochemistry (SABC and SP). RESULTS: Both EEP and WSP antagonized the lung edema, decreased the inflammation and inhibited the expression of CD54 and activation of NF-κB p65. CONCLUSION: The increase in the expression of CD54 and the activation of NF-κB p65 in the lung tissues of ALI were involved in the formation of ALI. Propolis ameliorated the lung damage, which maybe related to the inhibition of CD54 expression and NF-κB p65 activation.