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影响牛生长发育性状的GH基因遗传效应分析   总被引:12,自引:2,他引:10       下载免费PDF全文
 【目的】研究牛GH基因的遗传多态以及各多态位点对肉牛生长发育性状的影响,以期找到可用于标记辅助选择的分子标记,为下一步分子育种提供依据。【方法】利用PCR-SSCP和PCR-RFLP方法分析了牛GH基因第3内含子、第4内含子、第5外显子、3′非翻译区和5′侧翼区的位点遗传多态,并分析了5个多态位点不同基因型对肉牛体重、体高、体斜长、胸围和肉用指数的基因型效应,以及GH基因的第5外显子、3′非翻译区位点的不同基因型组合基因型效应。【结果】GH基因的第5外显子、3′非翻译区的基因型效应在肉牛体重、体高、体斜长、胸围和肉用指数性状上存在显著差异(P<0.05),且这2个位点等位基因B为优势等位基因;第5外显子和3′非翻译区的组合基因型ABBB(即组合5)的基因型效应(极)显著(P<0.05或P<0.01)高于其它6个组合,且多标记位点的组合效应值极显著高于单标记位点效应。【结论】GH基因第5外显子位点突变等位基因B为体重、体高、体斜长、胸围的优势等位基因。  相似文献
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长白猪主要选育性状间的典型相关分析   总被引:10,自引:0,他引:10       下载免费PDF全文
对长白猪的3组14个性状进行了典型相关分析。其中生长速度包含4个性状,体尺包含6个性状,膘厚和外貌评分等包含4个性状。结果表明,生长速度与体尺性状的第一和第二典型相关系数达极显著水平,分别为0.647 8和0.353 1,占总相关的96.52%。生长速度与膘厚和外貌评分等性状的第一和第二典型相关同样达极显著水平,分别为0.925和0.241 7,占总相关的99.9%。体尺与膘厚和外貌评分等性状只有第一个典型相关系数达极显著水平,其典型相关系数为0.328 7,占总相关的75.59%。分析还表明在这些性状中起主要作用的性状有X1(二月龄体重)、X3(测定结束体重)、X7(胸围)、X10(腿臀围)、X11(背膘厚)、X12(校正膘厚)。  相似文献
3.
黄牛Gli3基因第10外显子多态性及其与生长性状相关性   总被引:1,自引:0,他引:1  
利用PCR-SSCP和DNA测序技术研究708头黄牛 Gli3基因第10外显子的多态性,发现此外显子存在SSCP多态.对不同SSCP带型的对应片段进行测序分析,共发现2个新的SNP多态位点(NW_001494928:g.205649T>C,205754A>C),这2个SNP完全连锁.南阳牛不同基因型与生长发育性状的相关性分析显示,CC基因型个体的24月龄平均日增体质量指标显著大于TT基因型个体(P<0.05),其余各项指标基因型间差异不显著 (P>0.05).因此,该位点有可能作为南阳牛生长性状辅助选择的标记之一.秦川牛和郏县红牛群体中没有发现与该基因座多态显著相关的性状.  相似文献
4.
The objective of this study was to determine the genotype effects of the bovine insulin-like growth factor Ⅰ (IGF-Ⅰ) and its binding protein 3 (IGFBP3) genes on growth and development traits in beef cows, including 130 Chinese Simmental, 42 Nanyang, and 47 Luxi Yellow cattle. Sequence variations in the bovine IGF-Ⅰ and IGFBP3 genes were investigated by single strand conformation polymorphism (SSCP). SSCPs were detected in 6 fragments, which is the 5'-flanking region, the 2nd exon, the 5th exon, and the 5th intron of the IGF-1 gene, and the 2nd exon, the 3rd exon of the 1GFBP3 gene. Two polymorphisms, an A-to-G transition in the 2rid exon of the IGF-Ⅰ gene and a T-to-C transition in the 2rid exon of IGFBP3 gene were detected in 3 breeds. The allele frequencies of 2 polymorphisms were 0.0411 (A), 0.9589 (B), and 0.7237 (A), 0.2763 (B), respectively. These 2 loci were analyzed to associate with body weight, height at withers, body length, heart girth, rump width, and beef production index (BPI) at 0, 6, 12, 24, and 36-month old. The IGFBP3 locus was shown to be associated with rump width, heart girth at 24-month and 36-month. Animals with BB genotype had higher rump width (24.86 ± 0.47) cm at 24-month and (27.50 ± 0.63) em at 36-month. The heart girth was highest for the individuals with BB genotype (171.33 ± 1.84) cm and higher than those with AB genotype (166.68 ± 1.13) cm (P〈 0.05) at 36-month.  相似文献
5.
以5个地方黄牛及青海牦牛为研究对象,利用PCR-SSCP和DNA测序技术对367头黄牛与牦牛的类血管生长因子4基因的第5外显子(ANGPTL4exon5)序列进行SNP检测,并分析该SNP与黄牛与牦牛生长性状的相关性。结果发现1个新的SNP多态位点(NC007305:g.C4899T),并检测出CC、TT和CT3种基因型。黄牛和牦牛不同基因型与生长发育性状的相关分析显示,黄牛TT基因型个体的体质量(399.096±32.946)kg、体斜长(147.447±4.456)cm、胸围(175.188±5.814)cm和腹围(204.657±7.113)cm,显著大于CT基因型个体,依次为(275.636±51.266)kg、(126.470±6.933)cm、(154.031±9.047)cm、(178.911±11.069)cm,其余各项指标基因型间差异不显著。因此,该位点有可能作为黄牛生长性状辅助选择的标记之一。青海牦牛群体中没有发现与该基因座多态显著相关的性状。  相似文献
6.
利用PCR-SSCP和DNA测序技术,对秦川牛、南阳牛、郏县红牛和中国荷斯坦牛(共计817个个体)的LHX4基因外显子5及其两侧部分内含子序列进行多态性分析,并结合南阳牛的生长指标,分析不同基因型的遗传效应。新发现3处单核苷酸突变位点,分别是NW001493443:g.42757G>A、NW001493443:g.42768A>G和NW001493443:g.42917T>C,并因此形成AA、AB和BB 3种基因型。4个牛群体中,AA基因型的频率分别为0.766 2、0.771 70、.723 4和0.891 3。群体间的χ2检验结果揭示,不同牛品种的基因型频率差异不显著(P>0.05)。南阳牛群体中不同基因型个体与生长指标间的相关性分析结果显示,AA基因型个体的6月龄体质量显著大于其他基因型个体(P<0.05),暗示该位点有可能作为南阳牛生长性状辅助选择的标记之一。  相似文献
7.
The objective of this study was to determine the genotype effects of the bovine insulin-like growth factor I (IGF-I) and its binding protein 3 (IGFBP3) genes on growth and development traits in beef cows,including 130 Chinese Simmental,42 Nanyang,and 47 Luxi Yellow cattle.Sequence variations in the bovine IGF-I and IGFBP3 genes were investigated by single strand conformation polymorphism (SSCP).SSCPs were detected in 6 fragments,which is the 5'-flanking region,the 2nd exon,the 5th exon,and the 5th intron of the IGF-I gene,and the 2nd exon,the 3rd exon of the IGFBP3 gene.Two polymorphisms,an A-to-G transition in the 2nd exon of the IGF-I gene and a T-to-C transition in the 2nd exon of IGFBP3 gene were detected in 3 breeds.The allele frequencies of 2 polymorphisms were 0.0411 (A),0.9589 (B),and 0.7237 (A),0.2763 (B),respectively.These 2 loci were analyzed to associate with body weight,height at withers,body length,heart girth,rump width,and beef production index (BPI) at 0,6,12,24,and 36-month old.The 1GFBP3 locus was shown to be associated with rump width,heart girth at 24-month and 36-month.Animals with BB genotype had higher rump width (24.86±0.47) cm at 24-month and (27.50±0.63) cm at 36-month.The heart girth was highest for the individuals with BB genotype (171.33±1.84) cm and higher than those with AB genotype (166.68±1.13) cm (P<0.05) at 36-month.  相似文献
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