葡萄汁奶酒发酵工艺研究

本文以牛奶和葡萄为原料,通过单因素和正交试验优化了葡萄汁奶酒的发酵工艺。结果表明,影响葡萄汁奶酒感官评分因素的强弱顺序依次为发酵温度>接种量>发酵时间>蔗糖添加量>葡萄汁添加量,通过计算发现最佳组合为发酵温度25℃,发酵时间5d,接种量1.00%∶0.50%∶1.50%,蔗糖添加量8%,葡萄汁添加量11%。该组合酿制的葡萄汁奶酒感官评分最高,为92分。经测得,葡萄汁奶酒在最佳发酵工艺条件下的总糖为9.5g/L、酒精体积分数为6.8%,总酸质量浓度为7.2g/L,氨基酸态氮质量浓度为0.21g/L。该葡萄汁奶酒中的天门冬氨酸、谷氨酸、异亮氨酸、亮氨酸、赖氨酸和脯氨酸含量显著高于奶酒和牛奶中的含量(P<0.05)。     

浙西南山区巨峰葡萄平棚避雨栽培病害发生及防治

总结浙西南山区巨峰葡萄平棚避雨栽培主要病害的发生规律,并提出防治措施。     

朝阳市酿酒葡萄‘威代尔’虫害调查初报

作者针对朝阳地区酿酒葡萄主要虫害种类及发生规律进行调查研究,以便有针对性进行防治。调查研究发现,主要虫害种类有短须螨、蓟马和蒙古灰象甲,发生高峰期在早春,所以虫害防治重点在葡萄出土上架时期。     

蚯蚓堆肥和蚯蚓发酵液体肥在设施葡萄上应用研究

以设施栽培的‘红地球’葡萄为试验材料，采用单因素完全随机设计，研究了蚯蚓堆肥与蚯蚓发酵液体肥对葡萄叶片光合能力、植株生长情况、土壤状况和果实品质及产量的影响。结果表明：利用蚯蚓堆肥与蚯蚓发酵液体肥，提高叶绿素相对含量与净光合速率11.12%、15.31%，促进葡萄的生长，提高叶片面积、茎粗和新稍生长量7.58%、6.42%、18.86%，开花期促进茎叶磷素的吸收，成熟期促进茎叶钾素的吸收；改善土壤理化性质，降低土壤容重30.43%，显著增加总孔隙度8.63%，增加土壤EC值38.23%，pH值降低8.32%，提高速效氮、磷、钾10.56%、11.11%、12.67%，提升有机质3.15 g?kg-1；活化土壤微生物，提高脲酶、磷酸酶和蔗糖酶66.86%、54.60%、82.03%，细菌、放线菌、真菌数量是对照的3.20倍、0.43倍、3.28倍，提高微生物碳氮14.98%、20.23%，增加可溶性蛋白、Vc、可溶性固形物、糖酸比24.31%、9.74%、5.49%、23.46%，降低可滴定酸13.51%；产量增加了21.73%，并土壤理化性质以及土壤微生物关系密切。     

Effects of water infiltration and storage in cultivated soil on surface irrigation

Surface irrigation analysis and design require the knowledge of the variation of the cumulative infiltration water Z (L) (per unit area) into the soil as a function of the infiltration time t (T). The purpose of this study is to evaluate water infiltration and storage under surface irrigation in an alluvial clay soil cultivated with grape yield, and to determine if partially wetted furrow irrigation has more efficient water storage and infiltration than traditional border irrigation. The two irrigation components considered were wet (WT) and dry (DT) treatments, at which water applied when available soil water reached 65% and 50%, and the traditional border irrigation control. Empirical power form equations were obtained for measured advance and recession times along the furrow length during the irrigation stages of advance, storage, depletion and recession. The infiltration (cumulative depth, Z and rate, I) was functioned to opportunity time (t_o) in minute for WT and DT treatments as: Z_WT = 0.528 t_o^0.6, Z_DT = 1.2 t_o^0.501, I_WT = 19 t_o^−0.4, and I_DT = 36 t_o^−0.498. The irrigation efficiency and soil water distribution have been evaluated using linear distribution and relative schedule depth. Coefficient of variation (CV) was 5.2 and 9.5% for WT and DT under furrow irrigation system comparing with 7.8% in border, respectively. Water was deeply percolated as 11.88 and 19.2% for wet and dry furrow treatments, respectively, compared with 12.8% for control, with no deficit in the irrigated area. Partially wetted furrow irrigation had greater water-efficiency and grape yield than both dry furrow and traditional border irrigations, where application efficiency achieved as 88.1% for wet furrow irrigation that achieved high grape fruit yield (30.71 Mg/ha) and water use efficiency 11.9 kg/m³.     

1-MCP结合冰温贮藏对葡萄果实质地的影响

为探讨1-MCP处理结合冰温贮藏对采后葡萄果实质地的影响,以乍娜葡萄为试材,应用质构仪质地多面分析( TPA)方法对葡萄贮藏期间果肉质地参数变化规律进行研究.结果表明,1.0μ.L/L 1-MCP处理结合冰温贮藏可以延缓葡萄果实的软化,果实的硬度、弹性、凝聚性和咀嚼性较之单纯的冷库和冰温贮藏均有所提高;果肉硬度与回复性呈较好的正相关性,果肉弹性与果肉回复性、咀嚼性、凝聚性均呈现较好的相关性;葡萄好果率与果肉硬度在一定范围内呈良好线性关系.结果也表明,TPA测试能够较好地反映葡萄贮藏期间果实质地的变化,适用于葡萄贮藏品质的客观评价.     

根系分区交替滴灌条件下葡萄根系分布特征及生长动态

采用原位取土法和根系生态监测系统连续两年研究了葡萄的根系空间分布和全生育期根系生长动态,结果表明:葡萄根系在水平方向主要分布在距离树干100 cm的范围内,占到总根系的80％以上,而且在径向方向呈指数衰减;葡萄根系在垂直方向主要分布在0～60 cm范围的土层内,占到总根系的75％以上.根系分区交替滴灌条件下干燥区与湿润区根系生长是不同的,葡萄的新生根系受到土壤水分条件的限制和自身生长的影响.在整个生育期,葡萄根系分区交替滴灌两侧根系生长均呈抛物线变化.     

葡萄果实cDNA文库的构建及鉴定

构建葡萄果实cDNA文库是研究果实发育相关基因表达调控的基础工作.本实验从巨峰葡萄果实中提取了高质量的RNA,利用MMLV反转录酶把总RNA中的mRNA反转录成cDNA第一链,用特异引物进行LD-PCR扩增合成双链cDNA.将分级纯化的带有粘性末端双链cDNA与λTrip Ⅰ Ex2载体连接,重组载体体外包埋成为cDNA原始文库.初步鉴定原始文库的滴度为1.32×106 pfu/mL,文库扩增后滴度达1.45×1010 pfu/mL.从扩增文库中随机挑取250个克隆进行PCR鉴定,结果表明重组率为98.7%,插入片段大小在0.5～2.0 kb之间,因此,本研究成功构建了葡萄果实cDNA文库.     

葡萄籽原花青素生物学功能及其在畜禽生产上应用的研究进展

原花青素是一类广泛存在于植物界的多酚化合物的总称,其中以葡萄籽的含量最为丰富。研究表明,葡萄籽原花青素(GSP)有多种生物学活性。笔者综述了GSP的清除自由基、抗氧化、抗肿瘤、降血脂等生物学功能研究及其在畜禽生产上的应用研究进展,提出了将GSP作为新型饲料添加剂以提高动物的免疫力、肉品品质和抗应激作用,为GSP进一步开发和利用提供参考。