Anethole inhibits growth of recently emerged multidrug resistant toxigenic Vibrio cholerae O1 El Tor variant strains in vitro

To search natural compounds having inhibitory effect on bacterial growth is important, particularly in view of growing multidrug resistant (MDR) strains of bacterial pathogens. Like other bacterial pathogens, MDR Vibrio cholerae, the causative agent of diarrheal disease cholera, is becoming a great concern. As an approach of searching new antimicrobial agents, here, we show that anethole, a well-studied natural component of sweet fennel and star anise seeds, could potentially inhibit the growth of MDR O1 El Tor biotype, the ongoing 7th cholera pandemic variant strains of toxigenic V. cholerae. The minimum inhibitory concentration (MIC) of anethole against diverse O1 El Tor biotype strains is evaluated as 200 µg/ml. Moreover, the effect of anethole is bactericidal and exerts rapid-killing action on V. cholerae cells. This study is the first report which demonstrates that anethole, purified from natural compound, is a potent inhibitor of growth of toxigenic V. cholerae. Our data suggest that anethole could be a potential antimicrobial drug candidate, particularly against MDR V. cholerae mediated infections.     

异齿裂腹鱼人工规模化繁殖技术研究

2010年4～6月,对野生异齿裂腹鱼(Schizothorax o' connori)人工规模化繁殖技术进行研究,并初步进行产后亲鱼恢复培养技术研究.对108尾雌鱼进行干法人工授精,共采卵104万多粒,孵出仔鱼62万多尾.其中45尾雌亲鱼自然成熟,共采卵46.8万多粒,平均受精率和孵化率低于人工催产雌鱼卵.人工催产83...     

小样本情况下需水量预测模型研究

针对传统的GM（1,1）模型,分析了其预测结果与实际过程存在的偏差,通过GM（1,1）模型与自回归滑动平均模型相结合的方法以弥补偏差。以钦州市1999-2009年及2005-2009年城市供水总量这两组基础数据对本文所建模型进行验证,并利用此模型预测了钦州市2010-2018年需水量。通过与其他预测模型的对比,进一步证...     

近代中国蚕桑业发展与农民经济理性

本文从农家经营理性选择的角度,考察了近代中国蚕桑生产的发展,论述了近代中国农家兼业经济演化的新条件和新特点。     

奥尼罗非鱼及其亲本的微卫星分析

运用SSR技术对奥利亚罗非鱼（Oreochromis aureus♂）、尼罗罗非鱼（Oreochromis niloticus♀）及其杂交子代（O．aureus♂×O．niloticus♀）的遗传多样性进行了研究。从12对微卫星引物中筛选出8个微卫星特异性位点，其中奥利亚罗非鱼（♂）特异位点7个；尼罗罗非鱼（♀）5个；杂交子代6个。利用这8个位点检测3个群体的遗传结构，共检测到63个等住基因，平均等位基因2．4394个，奥利亚罗非鱼（♂）、尼罗罗非鱼（♀）和杂交子代的平均观测杂合度分别为O．7083、0．9015和0．9787；平均期望杂合度分别为0．5341、0．5761和0．6435，平均多态信息含量（PIC）分别为0．4133、0．4693和0．5584。结果表明，3个群体的遗传多样性水平都较高，其中杂交子代群体的遗传多样性水平最高，奥利亚罗非鱼群体遗传多样性最低；基于Nei＇s遗传距离的聚类分析显示尼罗罗非鱼（♀）和杂交子代的遗传距离较近。     

水磷耦合对小麦耗水特性和子粒产量的影响

在低磷地力、沙质壤土条件下,选用强筋小麦品种济麦20,设置全生育期不灌水(W0),灌底墒水+拔节水+开花水,每次灌水30 mm （W1）、60 mm（W2）、90 mm （W3） 4个灌溉处理;每个灌溉处理下设置不施磷(P0)、施P2O5105 kg/hm2（P1）、210 kg/hm2（P2）3个施磷量处理,研究了水磷耦合对麦田耗水特性、产量及水分利用率的影响。结果表明,1）在同一磷素水平下,随灌水量增加,小麦总耗水量增大,降水量和土壤供水量占耗水量的比例降低。综合考虑耗水量、产量、收获指数、水分利用率等指标,最优处理为P1W2处理,其次为P1W1处理,其总耗水量分别为435.5 mm、366.0 mm,灌水量、降水量和土壤供水量占耗水量的比例分别为41.3%、39.3%、19.3%和24.6%、46.8%、28.6%;开花至成熟阶段的耗水量占小麦全生育期耗水量的36.9%～43.3%,此阶段两处理的日耗水量、耗水模系数分别为4.6 mm、3.6 mm和42.3%、39.3%。2）施用磷肥,各处理的干物质积累量增加,子粒产量表现为W2、W3W1W0,W2、W3处理之间差异不显著;与不施磷肥的处理比较,显著提高了土壤供水量占总耗水量的比例。3）收获指数和水分利用率均为W1W2W3,P2水平下W1、W2、W3处理的收获指数和水分利用率均低于P1水平。以上结果表明,在本试验条件下,施磷（P2O5）105 kg/hm2(P1)、灌水180 mm(W2)的处理获得高产和较高的水分利用率;施磷（P2O5）105 kg/hm2（P1）、灌水90 mm（W1）的处理获得较高的产量,水分利用率显著高于上述处理,耗水量则显著低于上述处理,可供生产中水资源不足的情况下参考。     

Partial replacement of Fe(o,o‐EDDHA) by humic substances for Fe nutrition and fruit quality of citrus

The most widely used Iron (Fe) fertilizer in calcareous soils is the synthetic chelate Fe(o,o‐EDDHA). However, humic substances are occasionally combined with Fe chelates in drip irrigation systems in order to lower costs. We investigated the effect of various mixtures of Fe(o,o‐EDDHA) and a commercially available humic substance on Fe availability in a calcareous soil from Murcia, Spain (in vitro experiment) and on leaf Fe content and fruit‐quality attributes of Citrus macrophylla (field experiment). In the in vitro experiment, a calcareous soil was incubated for 15 d with solutions of sole Fe(o,o‐EDDHA) and humic substance and of a mixture of humic substance and Fe(o,o‐EDDHA) to determine the dynamics of available Fe. While the mixture did not significantly increase the available soil Fe, it did decrease the rate of Fe retention in the surface soil compared to sole Fe(o,o‐EDDHA). In the field experiment, the substitution in the application solution of 67% of Fe(o,o‐EDDHA) by commercial humic substance increased leaf P in lemon trees from 0.19% with sole Fe(o,o‐EDDHA) to 0.30% and leaf Fe from 94 mg kg^–1 to 115 mg kg^–1. Some quality parameters like vitamin C content and peel thickness were also improved with a partial substitution of Fe(o,o‐EDDHA) by humic substances. We conclude that a partial substitution of commercial Fe chelates by humic substance can improve crop Fe uptake and may thus be economically attractive. The underlying physiological mechanisms and ecological implications require further studies.     

土壤中碳酸盐的碳氧同位素分析

本文采用磷酸法分析土壤中碳酸盐的碳、氧同位素组成,对分析方法和制样装置作了叙述,比较了经不同预处理土壤的碳、氧同位素值,指出土壤有机碳的存在不影响碳酸盐同位素组成的精密测定,有氧灼烧预处理土壤样品对分析结果有明显影响,使δ~13C和δ~18O值降低。测定多种标准样品表明,此方法准确、可靠,重复制样测定的标准偏差在0.1‰以内。     

Biological effects of El Niño on the Galápagos penguin

Long-term monitoring of physical and biological parameters is essential for understanding the effects of El Niño on bird populations, particularly for small or declining populations. We examined the biological effects of El Niño activity from 1965 to 2004 using instrumental sea-surface temperatures from the Galápagos Islands and 20 years of census counts of the Galápagos penguin. Between 1965 and 2004, nine El Niño events were recorded of which two were strong and seven were weak. The two strong El Niño events of 1982-1983 and 1997-1998 were followed by crashes of 77% and 65% of the penguin population, respectively. The evidence suggests that the increased frequency of weak El Niño events limits population recovery. The 2004 penguin population is estimated to be at less than 50% of that prior to the strong 1982-1983 El Niño event. We discuss the biological effects of increased El Niño intensity and frequency within the context of a 6000-year record of El Niño influence and in the light of increasing anthropogenic threats operating after 1535, when the Archipelago was discovered by Europeans.