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1.
为解决草莓采后果实腐烂变质问题,将草莓置于甘草提取液、乳酸钙溶液、蒸馏水中浸泡1 min,取出晾干后常温贮藏,比较3组草莓失重率、腐烂率、可溶性糖、维生素C含量、超氧化物歧化酶SOD活性的变化。与对照组相比,贮藏7天后,甘草提取液组草莓失重率降低34.72%、腐烂率降低31.56%、可溶性糖含量提高99.10%、维生素C含量提高154.55%、超氧化物歧化酶SOD活性提高206.24%,乳酸钙处理和甘草提取液保鲜效果没有显著差异(P>0.05)。实验期内,2种处理方法均可提高草莓保鲜效果。 相似文献
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AIM: To explore the effect of inositol 1, 4, 5-trisposphate receptor (IP3R) in luteinizing hormone-epidermal growth factor receptor (LH-EGFR)-induced oocyte meiotic resumption. METHODS: Models of mouse cumulus-oocyte complexs (COCs) culture and follicle culture in vitro were generated to study the effects of 2-aminoethyl diphenyl borate (2-APB) and heparin (IP3R specific inhibitors) on LH/EGF-induced oocyte meiotic resumption and EGF-induced cumulus cell expansion. Real-time PCR was used to detect the mRNA expression of cumulus expansion-related factors. The changes of the intracellular calcium level were monitored using Fluo 3-AM, and the cGMP level was measured by ELISA. RESULTS: The inhibitors of IP3R, 2-APB and heparin, dramatically reversed EGF-induced oocyte maturation (P <0.05) and decreased cGMP levels in COCs (P <0.05). In addition, 2-APB and heparin reversed EGF-induced cumulus expansion, and significantly inhibited EGF-induced cumulus expansion-related factor expression (P <0.05). The activation of IP3R increased intracellular calcium level, and the study found that 2-APB and heparin dramatically reversed EGF-induced elevation of calcium level in cumulus cells (P <0.05). Follicular culture in vitro showed that 2-APB and heparin significantly reversed the LH-induced oocyte maturation (P <0.05). CONCLUSION: LH-EGFR signaling pathway increases calcium level in cumulus cells through IP3R, resulting in meiotic resumption. 相似文献
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近几年来‘王林’苹果苦痘病的发病率较高,为了探索防治办法,2018—2019年在瓦房店市驼山乡大魏村‘王林’苹果园进行施用“聚能螯合钙+硅”+生物有机肥试验。结果表明:第1年好果率达到90.2%,比对照提高15.0%;第2年分别为96.82%、22.23%。病果的病斑数也明显减少。 相似文献
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水培试验表明,稀土有平衡树体钙素营养的作用,在钙浓度低时,稀土能促进果树对钙的吸收,在钙浓度高时,可在一定程度上抑制果树对钙的吸收。田间喷钙可促进果树对氮、钙、锌的吸收,并增加果实中钙的浓度。稀土、钙混合液浸果可使果实中钙浓度增加0.165%,保鲜率比对照增加26%。 相似文献
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AIM: To investigate the effect of hesperetin on hypoxia/reoxygenation (H/R)-induced apoptosis in the H9c2 cells and to clarify the underlying mechanism. METHODS: The H/R model was established and the H9c2 cells were pretreated with hesperetin for 4 h. The cell viability and cell damage were measured by CCK-8 assay and lactate dehydrogenase (LDH) detection. The apoptosis was analyzed by Hoechst 33258 staining and flow cytometry. The intracellular calcium fluorescence intensity was measured by fluorescence microscopy and flow cytometry. The calcium-ATPase activity and the level of adenosine triphosphate (ATP) were measured by ELISA. The mitochondrial membrane potential was measured by JC-1 staining. The protein expression levels of Bcl-2, Bax and cytochrome C (Cyt-C) were determined by Western blot. RESULTS: Hesperetin reduced the apoptosis of the H9c2 cells induced by H/R, decreased intracellular Ca2+ fluorescence intensity, elevated Ca2+-ATPase activity, inhibited the mitochondrial membrane potential depolarization and increased the level of ATP (P<0.05). In addition, hesperetin significantly reduced the release of Cyt-C protein from mitochondria to cytoplasma and increased the Bcl-2/Bax ratio (P<0.05). After using the calcium ion inhibitor nimodipine, the percentage of the cells with mitochondrial membrane depolarization was decreased, the ATP level was increased and the protein expression of mitochondrion-related apoptosis molecules were decreased (P<0.05). CONCLUSION: Hesperetin reduces the apoptosis of the H9c2 cells induced by H/R, which may be related to inhibition of calcium overload and improvement of mitochondrial function. 相似文献
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Most of the soils of tropical countries are affected by erosion processes. As a result, much attention has been dedicated to the use of microorganisms to improve the geotechnical properties and stability of soils in the context of “bioengineering”. This work was carried out to analyze the effects of the use of a CaCO3 precipitating nutrient on native microbiota with the aim of mitigating the erosion processes in a tropical soil profile. We observed that the use of nutrient B4 enabled native bacteria present in the soil to precipitate calcium carbonate, resulting in improvements in the physical, chemical, mineralogical and mechanical properties of the soil, which allowed the mitigation of the erosion processes that characterize the soil profile studied. 相似文献
10.
AIM To investigate the effects of 17β-estradiol (E2) treatment on the mesenteric lymphatic microcirculation and isolated lymphatic contractility in rats after hemorrhagic shock, and to explore the relationship between contractility and the difference between intra- and extracellular calcium ion concentrations ([Ca2+]) of lymphatic smooth muscle cells (LSMCs). METHODS Male Wistar rats were divided into sham group, shock group and shock+E2 group. The rats were subjected to hemorrhage [(40±2) mmHg for 90 min] and resuscitation with or without subcutaneous injection of E2 (2 mg/kg). After resuscitation for 3 h, the mesenteric lymphatic microcirculation in vivo was observed. Moreover, the isolated mesenteric microlymphatic rings were prepared for the observations of lymphatic contractility evaluated by the indexes including end-systolic diameter, end-diastolic diameter, contraction frequency (CF) and passive diameter. Meanwhile, the difference between intra- and extracellular [Ca2+] of LSMCs was recorded during lymphatic contraction. RESULTS Treatment with E2 significantly enhanced the CF, total contractile fraction and lymphatic dynamics index in vivo in the rats after hemorrhagic shock, and increased the CF, the fractional pump flow and the difference between intra- and extracellular [Ca2+] of LSMCs in isolated lymphatics from the shocked rats (P <0.05). CONCLUSION Estrogen treatment enhances lymphatic contractility in rats after hemorrhagic shock, which is related to enhancement of difference between intra- and extracellular [Ca2+] of LSMCs. 相似文献