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1.
The potential reproduction power of domestic animals is limited by a complicated follicular atresia process. P53, caspase-9 (Casp9), Bax, Bcl-2 and Fas play a crucial role in the ovarian mitochondrion-dependent apoptosis and death receptor pathway. In accordance with this study, the expression levels of Casp9, Bax, Bcl-2 and Fas were analysed in ovaries and oviducts of yak by immunohistochemistry (IHC). P53 and the above in ovarian granulosa cells (GCs) from atretic (3–6 mm) to healthy follicles (6–8 mm) and in oviducts were examined from the luteal phase to the follicular phase during the oestrous circle by Western blot (WB) and real-time PCR (RT-PCR). Results demonstrated that typical classic apoptotic factors Casp9, Bax, Bcl-2 and Fas were expressed in the cytoplasm and zonal pellucida of oocytes, primordial follicles, primary follicles, ovarian surface epithelium, ovarian GCs, granular lutein cells, surface epithelia in oviduct uterotubal junction and oviduct ampulla during the luteal phase. RT-PCR and WB revealed that P53 and Fas significantly increased in GCs of atretic follicles. P53 and Casp9 increased in oviduct epithelium during the luteal phase, but Fas was unchanged. A contrary tendency was noted in Bcl-2 and Bax expression. Overall, P53 and Fas play an essential role in inducing GC apoptosis, and Bax, Bcl-2, Casp9 and P53 are involved in oviduct epithelial regeneration in yak.  相似文献   
2.
AIM: To investigate the preventive effects of Clostridium butyricum (C. butyricum) on the type of pylorus ligated gastric ulcer (GU) in mice and the underlying mechanisms. METHODS: ICR mice were randomly divided into 4 groups: sham operation group, model group, C. butyricum pretreatment group and omeprazole pretreatment group. Gastric pyloric ligation was adopted to establish GU model in mice. The gastric juice was collected to measure the content of gastric free mucus, the pH of gastric juice and the activity of pepsin. The gastric tissues were collected for routine HE staining to observe the pathological changes. The content of glycogen was detected by PAS staining. The protein expression of Bax and Bcl-2 in the gastric mucosa was also assessed by immunohistochemical staining. RESULTS: The HE and PAS staining showed that the C. butyricum pretreatment obviously attenuated the mucosa lesion induced by ligation. Compared with model group, the pH of gastric juice was significantly raised. The activity of pepsin fell off in C. butyricum group, which was lower than that in omeprazole group. In comparison with model group, the content of gastric free mucus was dramatically increased and PAS staining showed a significant rise in C. butyricum group, but not in omeprazole group. The protein expression of Bax was decreased and the protein expression of Bcl-2 was upgraded in C. butyricum group than those in model group. CONCLUSION: C. butyricum protects gastric mucosa against the challenge of pylorus ligation in mice and its mechanism may be related to inhibiting gastric acid secretion and the activation of pepsin, increasing the production of gastric free mucus, strengthening the expression of bcl-2 gene and inhibiting the expression of bax gene.  相似文献   
3.
Sclerotinia sclerotiorum is a destructive necrotrophic plant pathogen with global distribution. Although S. sclerotiorum has been studied extensively, substantial research on aspects of the pathogen's ability to cause disease is still needed. Bax inhibitor-1 protein functions as a suppressor of programmed cell death and is involved in the response to biotic and abiotic stress in animals, plants and yeast. In this study, we functionally characterized a putative Bax inhibitor-1 protein, Ss-Bi1, from S. sclerotiorum. Ss-Bi1 is predicted to contain a BAX inhibitor-1-like super family domain and shows significant homology with many BAX inhibitor-1 proteins. High expression levels of Ss-Bi1 were observed in hyphae under various stresses. Targeted silencing of Ss-Bi1 resulted in reduced virulence in host plants. Ss-Bi1 gene-silenced strains were more sensitive to heat stress and ER stress than the wild-type strain. The results suggest that Ss-Bi1 encodes a putative BAX inhibitor-1 protein that is required for full virulence of S. sclerotiorum.  相似文献   
4.
5.
The study was aimed to investigate the molecular mechanisms of miR-193a inducing apoptosis in cytopathogenic bovine virus diarrhea virus (cp BVDV) infected MBDK cells. Prediction of apoptosis-related miR-193a target gene Bax with bioinformatics online applications,TargetScan and Microcosm Targets,and verification of the interaction between miR-193a and Bax by dual-luciferase reporter assay were carried out to investigate the interaction between miR-193a and Bax. miR-193a overexpressing lentivirus pre-miR-193a-lv and lentivirus pre-miR-193a-inhibitor-lv inhibiting miR-193a expression were used to infect MDBK cells,respectively;Bax expression levels and apoptosis rates of MDBK cells were detected by Real-time quantitative PCR,Western blotting and flow cytometry,48 h later. According to the prediction and verification,we convinced that apoptosis-related gene Bax was the target of miR-193a;the results of Real-time quantitative PCR,Western blotting and dual-luciferase reporter assay showed that miR-193a directly targeted the miRNA response site of Bax 3'UTR and Bax expression levels were extremely significantly downregulated after miR-193a overexpression (P<0.01);meanwhile,the results of flow cytometry assay showed that apoptosis rates were extremely significantly decreased in MDBK cells infected with pre-miR-193a-lv (P<0.01). In a word,miR-193a could directly target Bax gene in pathway of apoptosis and promot apoptosis.  相似文献   
6.
The plasmid pGSA1285 was first modified by substituting its GUS sequence with the Chalcone synthase intron fragment from vector pFGC5941 to get the plant silencing expression vector that contained Kanamycin resistance site and was named as pGSA2285. Using PCR-based amplification, two different restriction sites at both ends of tobacco Bax inhibitor-1 (NtBI-1) gene were created, respectively, which made the construction of ihpRNA gene silencing vector more efficiently. Then, NtBI-1 genes were inserted into Multiple Cloning Site (MCS) of pGSA2285 respectively to form Bax inhibitor-1 ihpRNA gene silencing vector, named as pGSA4285, containing sense and anti-sense BI-1 sequence which was spliced by chalcone synthase intron. Combined PCR identification and enzyme restriction analyses, the results showed that Bax inhibitor-1 ihpRNA gene silencing vector had been constructed and transferred into Agrobacterium tumefaciens EHA105 successfully, which laid a foundation for the further study on the function of BI-1 in plant PCD regulation.  相似文献   
7.
AIM: To explore the effect of fucoxanthin (Fu) on the growth and apoptosis of HSC-T6 cells. METHODS: HSC-T6 cells were divided into blank control group, negative control group and drug groups (treated with different concentrations of Fu). The cell viability was detected by CCK-8 assay at 24 h, 48 h and 72 h after Fu treatment. The cell cycle distribution and apoptotic rate were analyzed by flow cytometry. The protein expression of Bcl-2 and Bax were detected by Western blot. RESULTS: Compared with blank control group, the viability of HSC-T6 cells was inhibited by Fu at concentrations of 15~75 μmol/L in a dose- and time-dependent manner (P < 0.01). The cell ratio of G1 phase was significantly decreased (P < 0.01) and the cell ratio of S phase and G2 phase was significantly increased (P < 0.01) in 60 μmol/L Fu group after 24 h. The cell ratio of G1 phase was significantly decreased (P < 0.05) and the cell ratio of S phase and G2 phase was significantly increased (P < 0.05) in 15 μmol/L and 30 μmol/L Fu groups in a dose-dependent manner after 48 h. The early cell apoptotic rates and total cell apoptotic rates were significantly increased in the Fu treatment groups in a dose-dependent manner (P < 0.05). The protein expression of Bax was significantly increased in the Fu treatment groups and the protein expression of Bcl-2 was significantly decreased in 30 μmol/L and 60 μmol/L Fu groups (P < 0.05).CONCLUSION: Fu inhibits the growth of HSC-T6 cells possiblely via arresting the cell cycle at S phase and G2 phase. The apoptosis of HSC-T6 cells induced by Fu might be via down-regulating the protein expression of Bcl-2 and up-regulating the protein expression of Bax.  相似文献   
8.
研究枸杞多糖(LBP)对双酚A(BPA)暴露小鼠睾丸生精细胞中Caspase-3、Bcl-2和Bax凋亡蛋白表达的影响.将50只成年雄性昆明小鼠随机分为A、B、C、D、E共5组,每组10只.除正常对照组(A组)注射等量橄榄油外,其余4组小鼠分别腹腔注射20 mg·kg 1的BPA,连续7d,建立生精损伤模型.同时C、D、E组小鼠分别灌服7d不同剂量的LBP(50、100、200 mg·kg-1),正常对照组(A组)和模型组(B组)小鼠灌服等量生理盐水.制备组织切片观察睾丸组织病理学变化,免疫组化法测定睾丸组织Caspase-3、Bax和Bcl-2凋亡蛋白的表达.结果显示,BPA可极显著增加睾丸生精细胞Caspase-3和Bax的阳性细胞数量(P<0.01),降低Bcl-2的表达(P<0.05).补充不同剂量LBP后,Caspase-3的阳性表达均极显著低于模型组(P<0.01).200 mg·kg-1 LBP组生精细胞Bax的阳性细胞数量极显著低于模型组(P<0.01);Bcl-2的表达随LBP剂量的增加而提高,其中200 mg·kg1LBP组阳性表达极显著高于模型组(P<0.01),Bcl-2/Bax比值也随着LBP剂量的增加而上升.结果表明,枸杞多糖通过调节凋亡相关基因的表达,抑制生精细胞凋亡,从而缓解双酚A引起的雄性生殖损伤.  相似文献   
9.
目的研究瓜萎燕白半夏汤对缺血再灌注损伤大鼠心肌细胞凋亡及Bcl-2 , Bax蛋白表达影响〔方法通过 结扎大鼠冠状动脉左前降支造成心肌缺血再灌注损伤模型,各组动物至实验时限心肌缺血30 min、再灌注90 min后, 取出心脏、采用TUNEL检测心肌细胞凋亡,免疫组化方法检测心肌Bcl-2 , Bax蛋白表达、结果与假手术组对照,模型 组细胞凋亡率及Bax表达水平均明显升高、Bcl-2表达水平降低,组间比较差异有统计学意义(P<0.01);瓜萎燕白半夏 能有效降低Bax表达、升高Bcl-2表达水平,抑制细胞凋亡的发生,与模型组比较,差异有统计学意义(P<0.01)、结论瓜 萎燕白半夏汤可能通过上调Bcl-2 ,卜调Bax蛋白表达而有效抑制心肌缺血再灌注损伤大鼠心肌细胞凋亡的发生  相似文献   
10.
AIM:To explore the effects of quercetin (Que) on the apoptosis of alveolar polymorphonuclear neutrophils (PMN) isolated from severe acute pancreatitis (SAP) rats with lung injury. METHODS:Forty-eight SD rats were randomly divided into 4 groups:sham group, SAP group, low-dose (50 mg/kg) Que group and high-dose (100 mg/kg) Que group. SAP was induced by retrograde administration of 5% sodium taurocholate into the biliary pancreatic duct. The SAP rats in Que groups were given quercetin, while the rats in sham group and SAP group received an infusion of physiological saline. Alveolar PMN were harvested by the collection of bronchoalveolar lavage fluid. The cell morphological changes were observed under fluorescent microscope. The cell apoptotic index was analyzed by flow cytometry. The protein levels of Bax and Bcl-2 were examined by Western blotting. Caspase-3 activity was measured by fluorescence spectrophotometry. RESULTS:Cell shrinkage and condensation of chromosomes were observed in alveolar PMN from SAP rats. Compared with sham group, the apoptotic index of alveolar PMN reduced in SAP group. The protein expression of Bax was significantly reduced, that of Bcl-2 was significantly enhanced, and caspase-3 activity was attenuated. After Que pretreatment, the apoptotic index of alveolar PMN increased, the protein expression of Bax was significantly enhanced, that of Bcl-2 was significantly reduced, and caspase-3 activity increased. The effects of Que presented a concentration-dependent manner, indicating that Que alleviated SAP-induced lung injury. CONCLUSION:The apoptosis of alveoar PMN is delayed in SAP rats. Quercetin induces apoptosis of alveolar PMN by up-regulating the expression of Bax and down-regulating the expression of Bcl-2.  相似文献   
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