USE OF 123IODINE METAIODOBENZYLGUANIDINE SCINTIGRAPHY FOR THE DIAGNOSIS OF A PHEOCHROMOCYTOMA IN A DOG

A 13-year-old neutered female Yorkshire terrier presented with a history of progressive episodic weakness and disorientation of 4 months duration. Physical and neurologic examinations were normal at presentation. Abdominal ultrasound revealed a mass involving the right adrenal gland. Standard planar scintigraphy was performed at 4, 18, and 24 hours after intravenous injection 185 MBq (5mCi) of ¹²³I-labeled metaiodobenzylguanidine (¹²³I-MIBG). An area of focal intense uptake was identified in the area of the right adrenal gland. A pheochromocytoma was confirmed histologically after surgical excision.     

BQ123对低温诱发的肉鸡肺血管重塑的影响

动态观察了肺小动脉中膜平滑肌的增殖及管腔面积的改变,探讨内皮素受体拮抗剂BQ123 对低温诱发的肉鸡肺血管重塑的影响。200只16日龄AA肉鸡随机均分为4组: 常温(20 ℃)对照组、低温(7~9 ℃)组、低温低剂量BQ123组和低温高剂量BQ123组。23日龄、30日龄时测定肺动脉压,并取肺组织做石蜡切片, 以Weigert间苯二酚复红染色,形态学计算机图象分析法测定肺细小动脉外径和内径、管总面积和管腔面积,计算中膜厚度占外径百分值(%)mMTPA及管壁面积/管总面积WA/TA(%)。结果显示:(1)BQ123 抑制低温肉鸡平均肺动脉压的升高,23日龄时低温高剂量BQ123组显著低于低温组(P<0 05),30 日龄时低温低剂量BQ123 组和低温高剂量BQ123组均极显著低于低温组(P<0 01);(2)BQ123 抑制低温肉鸡肺小动脉WA/TA(%)的升高,30~50μm的肺小动脉,低温组极显著高于低温低剂量BQ123 组及低温高剂量BQ123 组(P<0 01),其它分级的肺小动脉组间差异性与此类似;(3) BQ123抑制低温肉鸡肺小动脉的mMTPA的升高,30~50μm的肺小动脉,23日龄时低温组极显著高于低温低剂量BQ123 组和低温高剂量BQ123 组(P< 0 01), 30 日龄时低温组显著高于低温低剂量BQ123组(P<0 05)、极显著高于低温高剂量BQ123组(P<0 01),30μm以下的肺小动脉组间差异性与此类似,50~120μm     

基于Cortex-M4内核的μCOS-Ⅱ移植

针对TI公司新推出的基于Cortex-M4内核的TM4C123G高性能低功耗芯片,详细介绍了嵌入式开源实时操作系统μCOS-Ⅱ在芯片上的移植方法。根据移植的需求,首先介绍了芯片的一些基本功能以及相关的软件开发环境,然后结合芯片的固有特性以及μCOS-Ⅱ移植的需求,使用C语言和汇编语言修改了相关的源文件,并详细阐述了修改的原因。     

Construction of Full-length cDNA of Recombinant Rabies Virus SRV9 Strain Expressing EgM123 Gene of Echinococcus granulosus

The aim of the experiment was to construct the recombinant rabies virus SRV₉ vaccine strain with EgM123 gene by reverse genetics technology and provide the technical means for effective prevention and control of rabies and hydatidosis in China's agricultural and pastoral areas.In this study,the structural protein N,P and L genes of rabies virus SRV₉ were synthesized using gene synthesis technology,which was based on the complete genome sequence of rabies virus SRV₉ and the fusion fragment of the N-P-M fusion fragment and the rabies G gene,through the carrier of enzyme insertion connection methods,the recombinant rabies virus L gene,N-P-M gene fusion fragment and G+EgM123+eGFP gene fusion fragment were successively recombined on the expression vector pcDNA3.1(-) to construct the full-length cDNA of recombinant rabies virus SRV₉ with EgM123 gene.The synthesized genes were constructed on pcDNA3.1(-) expression vector,and the results of transformation,plasmid digestion and gene sequencing showed that the length of N,P,L,N+P+M and G+EgM123+eGFP gene fragments were 1 365,1 107,6 471,3 160 and 3 256 bp,respectively.The full-length cDNA fragment of EgM123 gene recombinant rabies virus full-length cDNA was 12 465 bp,and the sequencing results of each gene fragment were 100%.In this experiment,the full-length cDNA fragment of recombinant EgM123 rabies and eukaryotic expression vectors of the N,P and L genes of rabies virus were successfully constructed,which could save EgM123 gene recombinant rabies by reverse genetics,it also provided the reference for the development of rabies and hydatid disease combined gene recombinant oral live vaccine.     

In vitro effect of recombinant human tumor necrosis factor-alpha on canine neutrophil apoptosis

Neutrophils (polymorphonuclear leukocytes: PMNs) are essential for the host defense against various infections and are often injurious to the host, causing inflammatory diseases where tumor necrosis factor-alpha (TNF-alpha) is suggested to play an important role. Since an effect of TNF-alpha on canine PMN apoptosis has not been studied, canine PMNs were stimulated with recombinant human (rh)TNF-alpha in the present study to investigate the effect of TNF-alpha on canine PMN apoptosis. PMN apoptosis and function to produce ROS were assessed by flow cytometry. Delayed apoptosis was observed in the PMNs treated with rhTNF-alpha at 100 ng/ml, accompanied by retention of capability to produce ROS. However, PMN apoptosis was accelerated by rhTNF-alpha combined with cycloheximide. Therefore, it is indicated that TNF-alpha is able to activate anti- and pro-apoptotic pathways in PMNs and that the inhibition of PMN apoptosis by TNF-alpha requires protein synthesis in the PMNs.     

Use of a potentiometric vital dye to determine the effect of the herbicide bromoxynil octanoate on mitochondrial bioenenergetics in Chlamydomonas reinhardtii

BACKGROUND: The aims of the present study were to validate a vital mitochondrial potentiometric staining method in Chlamydomonas reinhardtii and to utilise this method to examine the effect of the herbicide bromoxynil octanoate on mitochondrial potential in this species. A range of stains was investigated, including Rhodamine 123, DASPMI, Mitotracker Green, Mitotracker Orange and JC‐1. RESULTS: Rhodamine 123 (R123) had the highest utility of several candidate stains. Incubation with both 5 and 10 µM carbonyl cyanide 3‐chlorophenylhydrazone caused significant fluorescence collapse [Dunn's post test (40.00, P < 0.01) and (45.49, P < 0.01) respectively], demonstrating that the R123 fluorescence reported mitochondrial potential. The effect of the herbicide bromoxynil octanoate was examined. Exposure to 0.1 mM of bromoxynil resulted in a significant increased mitochondrial fluorescence compared with the baseline (Mann–Whitney U = 222, P < 0.002), while concentrations of 1 mM and greater resulted in significant, almost complete loss of mitochondrial potential [mean fluorescence ratio = 1.193–1.289 (where a ratio of 1 represents total potential loss), Mann–Whitney U = 0.0, P < 0.001 (1 mM ), 0.0, P < 0.0001 (2 mM ), 0.0, P < 0.0001 (5 mM )]. EC₅₀ of the collapse in mitochondrial potential owing to bromoxynil incubation occurred at 0.72 mM , and the mean t₅₀ of bromoxynil octanoate action was 93 s. CONCLUSIONS: R123 is a sensitive potentiometric dye in C. reinhardtii that may find further use in investigations of both mitochondrial bioenergetics in plants and environmental toxicology. Copyright © 2011 Society of Chemical Industry     

中华绒螯蟹精子线粒体的检测

以中华绒螯蟹(Eriocheir sinensis)肌肉、鳃和精巢为对照,从线粒体标志酶SDH活性、线粒体特异性荧光探针(Rh123)以及线粒体16S rDNA PCR扩增3个途径,探讨了该蟹精子线粒体的存在状况。研究发现,肌肉和鳃SDH酶活性较高,精巢SDH酶活性显著低于肌肉和鳃,而精子SDH酶活性则无法检测到。使用Rh123检测发现,精巢中有较强的荧光,而精子中则无法观察到。通过GenBank中的中华绒螯蟹线粒体16S rDNA序列设计引物,利用PCR扩增方法,以beta-actin做内参,检测了中华绒螯蟹肌肉、鳃、精巢和精子中线粒体16S rDNA扩增情况,发现各组织或细胞beta-actin扩增片段大小、条带宽度和亮度基本一致,表明各模板DNA量基本一致;而在同一DNA浓度下,16S rDNA的扩增片段长度虽一致,但其产物量存在明显差异,精子16S rDNA产物量显著低于其他3种组织,其条带宽度和亮度很弱;16S rD-NA扩增产物经测序分析及比对证明与已有序列完全吻合。上述结果表明,中华绒螯蟹精子中存在线粒体,但其线粒体的数量极显著低于精巢和其他组织,这也是利用灵敏度较低的SDH酶活性和Rh123探针以及常规的组织学和超微结构观察法难以检测到其精子线粒体的原因。[中国水产科学,2007,14(1):139-143]     

内皮素A受体拮抗剂BQ123对肉仔鸡股动脉压和肺动脉压的影响

为探讨内源性内皮素-1(ET-1)是否参与肉鸡血压的维持等生理调节活动,采用急慢性给予内皮素ETA受体的特异性拮抗剂BQ123,研究内源性ET-1对正常肉鸡股动脉压和肺动脉压的影响。结果表明:1)放免法检测2~6周龄正常肉仔鸡血浆(从(40.82±1.90)升高到(93.70±11.19)pg/mL)和肺匀浆内皮素(从(503.84±27.40)升高到(701.04±16.18)pg/g)的含量随日龄的增加有升高的趋势;2)急性翼静脉注射BQ123(0.4和2.0μg/kg)能够极显著降低肉鸡的股动脉收缩压、舒张压和肺动脉收缩压、舒张压(P<0.01),注射2.0μg/kg BQ123压力下降程度比注射0.4μg/kg大,肺动脉压达到最低点的时间比股动脉早;3)慢性给予BQ123能够使肉鸡肺动脉收缩压极显著下降(对照组(3.37±0.47)kPa,BQ123组(2.46±0.50)kPa,P<0.01),股动脉收缩压显著下降(对照组(20.95±1.17)kPa,BQ123组(18.85±2.05)kPa,P<0.05)。试验结果显示:急性和慢性注射ETA受体的特异性拮抗剂BQ123都能降低肉鸡股动脉压和肺动脉压,推测内源性ET-1参与了肉鸡血压的维持等生理调节活动。     

BQ123对肉鸡腹水综合征的预防作用研究

通过动态观察肺动脉压、红细胞压积、腹水心脏指数、肺小动脉中膜厚度占外径百分值(mMTPA)及管壁面积/管总面积(WA/TA)的变化,探讨高选择性内皮素A受体拮抗剂BQ123对低温诱发肉鸡腹水综合征的预防作用。结果显示,BQ123能显著降低低温诱发肉鸡的肺动脉压、红细胞压积及腹水心脏指数(P0.05或P0.01);BQ123能显著减小低温诱发肉鸡的mMTPA及WA/TA(P0.05或P0.01)。结果表明,BQ123明显抑制了低温诱发肉鸡的肺动脉高压和肺血管重塑的形成和发展,对肉鸡腹水综合征具有明显的预防作用。