全文获取类型
收费全文 | 36024篇 |
免费 | 3219篇 |
国内免费 | 2051篇 |
专业分类
林业 | 3164篇 |
农学 | 3447篇 |
基础科学 | 979篇 |
3234篇 | |
综合类 | 15342篇 |
农作物 | 3282篇 |
水产渔业 | 2248篇 |
畜牧兽医 | 5548篇 |
园艺 | 2787篇 |
植物保护 | 1263篇 |
出版年
2024年 | 48篇 |
2023年 | 489篇 |
2022年 | 991篇 |
2021年 | 1147篇 |
2020年 | 1237篇 |
2019年 | 1099篇 |
2018年 | 959篇 |
2017年 | 1347篇 |
2016年 | 1597篇 |
2015年 | 1839篇 |
2014年 | 2204篇 |
2013年 | 2378篇 |
2012年 | 2926篇 |
2011年 | 2652篇 |
2010年 | 2129篇 |
2009年 | 2181篇 |
2008年 | 1927篇 |
2007年 | 2188篇 |
2006年 | 1969篇 |
2005年 | 1653篇 |
2004年 | 1083篇 |
2003年 | 919篇 |
2002年 | 731篇 |
2001年 | 695篇 |
2000年 | 600篇 |
1999年 | 536篇 |
1998年 | 449篇 |
1997年 | 403篇 |
1996年 | 385篇 |
1995年 | 347篇 |
1994年 | 318篇 |
1993年 | 325篇 |
1992年 | 291篇 |
1991年 | 268篇 |
1990年 | 240篇 |
1989年 | 183篇 |
1988年 | 140篇 |
1987年 | 108篇 |
1986年 | 67篇 |
1985年 | 59篇 |
1984年 | 44篇 |
1983年 | 22篇 |
1982年 | 23篇 |
1981年 | 29篇 |
1980年 | 18篇 |
1979年 | 15篇 |
1978年 | 6篇 |
1977年 | 9篇 |
1976年 | 7篇 |
1955年 | 5篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
1.
2.
The quality and safety of agricultural products is the technique focus of the strategy of rural vitaliza⁃tion. Carbamate pesticide residues are seriously threatening the safety of agricultural product and human life and health. Nowadays, the common detection methods are instrument-based for carbamate pesticide residue, which dis⁃ satisfy the request of an on-site rapid, sensitive and simultaneous detection for multiple carbamate pesticide resi⁃ dues. Based on the home-made monoclonal antibodies against carbofuran and carbaryl respectivity, a microarray for rapid and simultaneous detection of multiple carbamate pesticide residues was developed within 30 min. Under the optimized conditions, the limits of detection(LODs)were found to be 0.11 and 0.09 ng/g, while the linear ranges were 0.33-3000 and 0.18-1500 ng/g for carbofuran and carbaryl respectively. By using spiked peanut samples, it recorded recoveries of 88.4%-98.3%, 90.1%-118.8% for carbofuran and carbaryl respectively. The RSD of intraarray were found at 9.0%, 3.5%, while the RSD of inter-array were recorded at 12.3%, 16.3% respectively. The de⁃
tection results of the microarray were highly consistent with that of HPLC-MS/MS. This proposed microarray provid⁃ ed an on-site high-sensitivity, simultaneous and rapid detection of carbofuran and carbaryl, and could be widely used in the fields of agricultural product quality safety, food safety and environmental monitoring. 相似文献
3.
背主动脉注射是生产转基因鸡的经典方法,该方法不需换壳培养,但壳内注射技术难度大,并且无法实时观察后期胚胎发育情况。本实验对背主动脉注射法进行了改进,将增强型绿色荧光蛋白(EGFP)腺相关病毒(Adeno-associated Virus,AAV)壳外注射至150枚HH 14~16期(Hamburger-Hamilton Stage 14~16)鸡胚背主动脉中,再进行换壳培养,继续孵化至出壳,观察和分析改良背主动脉注射法与传统背主动脉注射法和胚盘下腔注射法对发育8、14、18、21 d鸡胚存活率、孵化率以及EGFP阳性检出率的影响。结果表明:改良背主动脉注射组鸡胚存活率均高于传统背主动脉注射组与胚盘下腔注射组(P<0.05或P<0.01);改良背主动脉注射组的鸡胚孵化率(37%)高于传统背主动脉注射组(16%)与胚盘下腔注射组(28%)(P<0.01);荧光蛋白手电筒检测显示,改良背主动脉注射组鸡胚EGFP阳性率(17%)明显高于传统背主动脉注射组(13%)与胚盘下腔注射组(12%)(P<0.01)。综上,背主动脉壳外注射结合换壳培养提高了转基因鸡胚胎孵化率和EGFP阳性检出率,对提高转基因鸡效率具有重要价值。 相似文献
4.
5.
以重庆市江津区为研究区,采集2019年生产的63份大米籽粒和64份玉米籽粒,用电感耦合等离子质谱仪(ICP–MS)和原子荧光光谱仪(AFS)测定大米和玉米籽粒中砷(As)、钡(Ba)、镉(Cd)、铜(Cu)、铁(Fe)、锰(Mn)、钼(Mo)、镍(Ni)和锌(Zn)的质量分数,并利用环境健康风险模型评价江津区大米和玉米中重金属的健康风险。结果表明:江津区大米和玉米籽粒中As最大质量分数分别为176.0、162.8 μg/kg,低于国家食品卫生As标准(<200 μg/kg);江津区3份大米和6份玉米样品中Cd质量分数超过国家食品卫生Cd标准(大米Cd质量分数<200 μg/kg、玉米Cd质量分数<100 μg/kg);大米籽粒中As和Mo质量分数均值显著高于玉米籽粒的(P<0.05),Cd质量分数均值略高于玉米籽粒的;大米籽粒中As、Cu、Mn和Zn的单因子污染指数均小于1,属于清洁水平,3份大米籽粒样品中Cd属于轻度污染;大米和玉米籽粒重金属综合污染指数分别为1.09和1.57,属于轻度污染,江津区玉米籽粒中重金属污染程度大于大米籽粒;大米籽粒中重金属As和Cd的致癌风险分别占健康总风险的82.7%和16.8%,表明江津区大米籽粒中As含量虽低于国家食品卫生标准安全值,但其健康风险较高,其次为Cd。可见,重庆地区需重点监控农作物中As和Cd的健康风险。 相似文献
6.
以福建特色水果‘油㮈’为试材,在等肥料成本条件下,进行水肥一体化施肥和常规施肥下‘油㮈’树枝梢生长长度和粗度、果实品质、产量表现及施肥成本的比较研究,以期为山地水肥一体化施肥提供参考。结果表明,水肥一体化施肥可在一定程度上促进当年枝梢长度和粗度的增长,提高果实可溶性糖、可溶性固形物及Vc含量,降低可滴定酸含量,提高果实平均单果重、可食率、产量。等价施肥条件下,水肥一体化施肥节省人工成本1104.0元/hm2。建议结合降水量等气候条件及山地土壤特点、生态环境等对水肥一体化施肥技术进行优化。 相似文献
7.
8.
《茶经》是我国茶文化形成的标志性著作,蕴含了丰富的生态哲学思想,主要体现在“天人合一、物我同源”的“一体观”、“崇敬自然、遵循规律”的“自然观”、“天人调和、平和淡然”的“中庸观”以及“精行俭德、清心御欲”的“道德观”四个方面。在全球生态危机背景下,深入分析《茶经》的生态哲学内涵,并尝试从“整体观”、“系统观”、“自然观”等角度寻求其与马克思主义生态思想的契合,实现传统生态智慧与现代生态理论的结合,对当下生态文明的建设具有重要意义。 相似文献
9.
To identify the major factors that contribute to the difference in lodging among different rice varieties under machine transplanting and their responses to nitrogen(N), field experiments were conducted at Danyang County(a representative eco-site of the Lower Yangtze River) in Jiangsu Province, China in 2017 and 2018, 22 hybrid indica varieties(HIs), 22 inbred japonica varieties(IJs) and two indica japonica hybrid varieties(IJHs) were transplanted by machine with three N rates(N0, N150 and N300, 0, 150 and 300 kg ha–1, respectively). Lodging-related physical parameters, morphological characteristics and apparent transport rates of dry matter were examined. Significant difference in yield was observed among different types of rice, and followed by IJsHIsIJHs. The average lodging index(LI) of hybrid varieties(HIs and IJHs) was higher than that of the inbred varieties(IJs) with higher plant height; moreover, lower apparent export rate of dry matter resulted lower LI in IJHs than in HIs. The HIs had a large difference in the LI, which came from the difference in bending stress(BS) induced by the difference in the apparent export rate of dry matter, varieties with lower leaf angle of upper three leaves possess strong lodging resistance capacity; however, the gap among the IJs was due to the difference in the cross section modulus(Z). The LI in the IJs or IJHs increased slightly with the increased N application, and there was no lodging incidence under the high N level, which was due to the low leaf angle and barely changed under high N; there was a significant interaction between varieties(HIs) and N rates in lodging rate and LI, varieties with lower leaf angle of upper three leaves were resistant to high N. These results suggest that compact plant type rice has higher lodging and N resistance at machine-transplanting method. 相似文献
10.
Shutao Dai Jinna Hou Maomao Qin Ziju Dai Xiaojie Jin Shilei Zhao Yanhua Dong Yongxia Wang Zhengqing Wu Zhensheng Lei 《中国油料作物学报(英文)》2021,6(1):28-34
Miniature inverted-repeat transposable elements(MITEs)are a group of DNA transposable element(TE)which preferentially distributed with gene associated regions.Tens of MITEs families have been revealed in Brassica napus genome,they scatter across the genome with tens of thousands copies and produce polymorphisms both intra-and inter-species.Our previous studies revealed a Tourist-like MITE,Monkey King,associated with vernalization requirement of B.napus,however there are still few studies reveal MITE association with agricultural traits in B.napus.In the present study,80 polymorphic markers were developed from 55 MITEs,and used to evaluate genetic diversity in a panel of B.napus accessions consisting of 101 natural and 25 synthetic genotypes.Five agricultural traits,oil content,glucosinolate content,erucic acid content,weight of thousand seeds(WTS)and plant height,were investigated across 3-years field experiments,in addition,two traits,hypocotyl length and root length,were evaluated at the 4-leaf stage in the laboratory.Correlations between the MITE-based markers and seven traits were analyzed,finally,10 polymorphic markers produced by 6 pairs of MITE specific primers were revealed relatively high correlation with 5 traits.Two polymorphic markers were anchored with two candidate genes,BnaA02g13530D and BnaA08g20010D,respectively,which may contribute to glucosinolate content and WTS.This research may contribute to genetic improvement through utilization of MITE-induced polymorphisms in Brassica species. 相似文献