Priliminary analysis of microRNA expression profiles of side population cells in human colon cancer cell lines

AIM：To investigate the role of side population (SP) cells in multidrug resistance of colon cancer cells and microRNA biomarkers of SP cells in colon cancer cells. METHODS：SP cells in colon cancer cells were sorted by flow cytometry. The cell viability was measured by MTT method. MicroRNA expression profiles were detected by microRNA chip. MicroRNA expression was verified by real-time PCR. RESULTS：The ratios of SP cells in HCT-15, HT-29 and LoVo colon cancer cell lines were 16.75%, 13.02% and 9.52%, respectively. In all 3 colon cancer cell lines, IC50 of the antitumor drugs including 5-fluorouracil, oxaliplatin and adriamycin for the SP cells were significantly higher than those for non-SP cells (P<005). MicroRNA profiling showed that miR-5000-3p, miR-5009-3p and miR-552 were all up-regulated in the SP cells of all 3 colon cancer cell lines. This result was verified by real-time PCR. CONCLUSION：miR-5000-3p, miR-5009-3p and miR-552 are all up-regulated in the SP cells of colon cancer cell lines, and may be the potential microRNA biomarkers of SP cells in colon cancer.     

基于ADAM-5000模块与MCGS的温室监控系统设计

针对某花卉大棚的控制要求,介绍了基于ADAM-5000模块的温室监控系统.现场数据的采集和控制使用ADAM-5000系列智能模块,上位机采用研华工控机IPC610,使用MCGS组态软件编制监控程序.为适应不同作物的生长需求,系统提供了专家数据库以满足不同作物的需求.该系统配置简单灵活,便于更改、升级;软件界面友好,易于操作,现场运行稳定.