普通硅肥与纳米硅肥对烤烟内生真菌种群结构的影响

To explore the effects of common silicon fertilizer and nano-silicon fertilizer on the population structure of endophytic fungi in flue-cured tobacco,Yunyan 99 was used as test material, common silicon and nano-silicon were sprayed at vigorous growth,budding and dome stages.The treatments were set as follows: CK (water), T5 (common silicon,0.17 kg/hm²), T8 (nano-silicon,0.32 kg/hm²).The effects of different silicon fertilizer on the diversity, composition, structure, chemical composition and aroma substance of endophytic fungi in flue-cured tobacco were studied. Compared with CK, T5 and T8 treatment could effectively improve the community diversity,composition, structure and the content of chemicals and aroma substances. Compared with CK, the diversity of endophytic fungi increased under T8 treatment and decreased under T5 treatment.Compared with CK, the relative abundance of Basidiomycota under T5 and T8 increased by 58.14% and 37.35%, respectively,at the same time, the relative abundance of Flobasidium increased by 113.17% and 417.07%, respectively.Among them, T8 treatment increased the species of endophytic fungi, among which,31 species were more than CK treatment. The correlation analysis was based on chemical composition, aroma substances of tobacco leaves and endophytic fungal community composition.The contents of nicotine,soluble protein,Maillard reaction products and aroma substances were significantly related to the community composition of endophytic fungi in tobacco leaves. The diversity of endophytic fungi in tobacco leaves could be increased by spraying nano-silica fertilizer on leaf surface,and the content of chemicals and aroma substances of tobacco could be changed.It is meaningful for human to produce high quality flue-cured tobacco.