Shikonin reverses HGF-induced resistance to gefitinib in lung cancer cells HCC827

AIM: To investigate the effect of shikonin on reversing hepatocyte growth factor(HGF)-induced resistance to gefitinib in lung cancer HCC827 cells, and to explore its possible mechanisms.METHODS: The gefitinib-resistant HCC827 cells induced by HGF were treated with shikonin and gefitinibthe alone or in combination. The inhibition rates of cell viability were determined by MTT assay. The invasive ability of HCC827 cells with HGF-induced resistance to gefitinib was determined by Transwell assay. The protein levels of epithelial-mesenchymal transition (EMT) and related signaling pathway in the HCC827 cells were detected by Western blot.RESULTS: The results of MTT assay showed that the cell activity of HCC827 cells was significantly inhibited by shikonin in a dose dependent manner. The IC₅₀ of shikonin in HCC827 cells was 3.06 μmol/L. And the IC₅₀ of gefitinib in HCC827 cells was 0.51 μmol/L. Under the condition of combined treatment with shikonin and gefitinib in the presence of HGF (20 μg/L), the IC₅₀ of gefitinib was 7.36 μmol/L, significantly lower than that treated with gefitinib alone (P<0.01), so did the result of the cell migration (P<0.01). HGF induced EMT, while shikonin reversed this effect. The protein expression level of p-AKT was significantly up-regulated by HGF, while markedly down-regulated treatment with shikonin and gefitinib compared with gefitinib alone (P<0.01).CONCLUSION: Shikonin reverses HGF-induced resistance to gefitinib in lung cancer HCC827 cells, and the mechanism may be likely related to the preventon of EMT and the inhibition of HGF-induced activation of p-AKT signaling pathway.     

渭南市种植业面源污染现状、防治难点及对策建议

掌握面源污染现状是进行污染防治的前提。本研究概述了渭南市种植业面源污染的现状,指出化肥施用高量低效并存、农药包装物污染、残膜污染持续以及秸秆综合利用衍生问题突出,是当前种植业面源污染亟待解决的问题,然后结合防治难点,从宣传普及、强化监测、健全法规、资金保障以及做好技术研发和推广等角度提出了较系统的防治对策和建议。     

关于忽必烈在宫廷建植草坪之事的考证

目前,广泛使用的硕士研究生、本科、高职高专草坪学教材及参考书,在回顾中国草坪利用史的时候,都会将元世祖忽必烈在宫廷内院建植草坪之事作为一个例证。然而经对原始文献的相关记载进行研究后,发现这种说法并不属实,实际情况是只种了一株沙蒿,最多也就是在台基上栽植了为数不多的牧草,并不能称其为草坪。元世祖在宫廷种植草坪、铺设草坪的说法,产生于对历史文献记载的误读或者揣测,在以后进行教材及参考书的修订和重编时,应予以纠正。     

水产养殖专业英语课程的教学改革与实践

水产养殖专业英语是为了适应社会经济发展而为学生开设的一门考查课,其目的是让学生掌握一种获取更多资料和信息的语言工具.然而,我国至今没有一本适合普通高职院校学生的专业英语教材.根据自身教学实践,本文从教学内容选择及教学手段改革两方面进行了细致深入的阐述,希望对我国水产养殖专业英语或其他学科专业英语的课程教学改革有所启发.     

气候变化背景下的中国林业建设

介绍了林业在应对气候变化国际进程中的重要地位,回顾了我国林业在应对气候变化背景下所取得的成就,提出了充分发挥森林碳汇潜力,科学应对气候变化的具体措施.     

Reinforcement Treatment on the Uneven Subsidence of Foundation

There are several methods to cope with the common problem of the uneven subsidence of foundation. The reason for uneven settlement of foundation of the frame structure was analyzed in this paper. Considering the limitation of the construction site and the principle of consolidation, a feasible reinforcement measure was taken. Meanwhile, a practical construction pattern was chosen upon the comparison of the reinforcement technology analysis and the construction method. The observation of the settlement result after consolidation of the structure shows that the result of this reinforcement treatment pattern is effective.     

幼犬佝偻病的诊断与防治

幼犬佝偻病是指由于维生素D缺乏及钙磷代谢障碍所致的骨营养不良.本病多发于1月龄～3月龄幼犬,临床上主要以生长发育迟缓、消化紊乱、骨骼发育异常等为特征.论文结合临床,就该病的诊断方法和防治措施进行总结与讨论.     

黄淮海地区夏玉米农艺性状与产量的通径分析

为应对当前黄淮海地区农业用水紧缺的情况, 选育和推广高水分利用率的高产夏玉米品种是一项重要措施。本试验采用完全随机设计, 对黄淮海区域11 个主推夏玉米品种在灌溉1 水条件下的农艺性状、产量和水分利用率进行通径分析与主成分分析, 结果表明: "滑丰8 号"、"蠡玉18"、"浚单20"和"冀玉3 号"的产量均超过10 000 kg·hm^-2, 且这4 个品种的水分利用率均在3.0 kg·m^-3 以上, 属于水分利用率高的超高产品种; "源申213"和"中科11"产量超过9 000 kg·hm^-2, 其水分利用率也均大于2.7 kg·m^-3, 属水分利用率较高的高产品种;"浚单18"、"登海662"、"冀农1 号"和"登海超级玉米"产量均超过8 000 kg·hm^-2, 其水分利用率在2.3~2.7 kg·m^-3之间, 属稳产品种。主成分分析及综合评价排名的结果表明, "冀玉3 号"、"蠡玉18"、"滑丰8 号"和"浚单20"4 个品种综合表现优异, 可以作为抗旱节水高产品种在黄淮海地区大面积推广。通径分析结果表明, 7 个主要农艺性状对产量的综合效应排名为: 芯重＞百粒重＞行粒数＞秃尖长度＞粒长＞棒长＞行数。芯重、行粒数和百粒重对产量的直接正效应最大, 秃尖长度对产量的直接负效应最大。以上结果为黄淮地区抗旱节水高产玉米品种选育和推广提供了重要信息。     

梅花鹿的生态生物学研究进展

梅花鹿（Cervus nippon）隶属偶蹄目鹿科,是濒危级珍稀保护动物,主要分布在黑龙江、吉林、江西、浙江、安徽、四川和甘肃省等地。总结了梅花鹿的分类与分布、种群密度和结构、生境选择、行为、繁殖、食性以及生存现状,提出了相应保护对策。     

不同饲料饲喂断奶仔猪对比试验研究

为了给断奶仔猪选择合适的饲料类型,采用2种类型的饲料对60头35日龄断奶的仔猪进行饲养试验。试验分为2组,即颗粒饲料组和粉状饲料组,每组三个重复,经30 d的饲养试验,结果表明：颗粒饲料组仔猪体质量每日增加数、日头均耗料量明显高于粉状饲料组（P〈0.05）,且颗粒饲料组的经济效益要高于粉状饲料组,而饲料转化效率没有明显差异（P〉0.05）,说明颗粒饲料比粉状饲料更适合饲喂断奶仔猪.