耕作措施对坡耕地红壤地表径流氮磷流失的影响

云南山地面积约占全省土地总面积的94%,特殊的地形特征,极易引发坡面土壤侵蚀和养分流失,严重影响了农业可持续生产。采取有效的农艺措施来减少坡耕地土壤养分流失是十分有必要的。为此,通过4年定位试验对顺坡(2组处理)、横坡(2组处理)2种耕作方向的复合农艺措施处理进行研究。结果表明:云南坡地红壤的径流时间主要集中在6—9月,且产流雨量占年降雨量的65.62%～75.82%。产流雨量与年降雨量呈现一致趋势。径流量和产流雨量呈线性关系(R_(NVF)~2=0.597 7,R_(OVF)~2=0.415 1,R_(OHF)~2=0.378 2,R_(OHFR)~2=0.335 5),其相关性大小顺序为顺坡处理横坡处理,不施肥处理施肥处理,覆膜处理揭膜处理(P0.01)。大雨(25～49.9 mm)和暴雨(≥50 mm)造成了年度大部分径流和养分的流失。横坡垄作组处理产生地表径流(177.13±28.87)～(182.28±33.75) mm,径流中总氮流失量(7.66±2.51)～(7.85±1.92) kg/hm~2,总磷流失量为(0.91±0.26)～(1.09±0.27) kg/hm~2,与顺坡垄作OVF(常规处理)相比径流和养分极显著减少了49.57%～52.13%,33.16%～53.88%(P0.01)。不同耕作措施下,复合耕作模式(优化施肥+横坡垄作+旺长期揭膜)拦截径流和养分流失的效果最好。RDA分析结果发现,与降雨量相比较,径流量是影响氮磷养分变化的主要环境因子(P0.01)。径流量与氮养分(NH_4~+-N除外)流失量的相关性高于与磷的相关性,表明氮比磷更容易随径流流失。顺坡处理NVF和OVF与环境因子径流量和氮磷养分流失量TN、TDN、NO_3~--N、NH_4~+-N、TP、TDP呈正相关,横坡处理OHF和OHFR与其呈负相关。     

云南松林下栽培滇黄精试验初报

[目的]探索云南松林下栽培滇黄精的技术。[方法]按照不同林分、坡位、密度设置样方,统计不同种苗类型、林龄类型、坡位类型、密度类型对林下栽培滇黄精生长的影响。[结果]在云南松林下栽培滇黄精,应选择块茎苗进行催芽处理,种植于排水较好的中坡位,郁闭度在0.6左右。[结论]为云南松林下栽培滇黄精提供了科学的依据。     

桔小实蝇在云南蒙自地区的发生规律研究

桔小实蝇寄主范围广、繁殖量大、危害重,是世界性的重要检疫害虫。本文通过调查分析,研究了云南蒙自市的海拔、防治措施、果树种类三因素对桔小实蝇种群动态的影响。结果表明,桔小实蝇在1412 m低海拔地区的种群数量大于1608 m和1700 m高海拔地区的,这说明海拔越高,桔小实蝇种群数量越小,危害也越小。根据在相似海拔地区相同果园桔小实蝇种群数量的比较,发现严格按照本课题组探索的防治措施做好桔小实蝇防治能收到良好的效果。蒙自市的水果成熟期不同,随着各种水果的成熟,桔小实蝇种群动态会出现多个峰值,3~5月桔小实蝇种群高峰期主要出现在枇杷园,7~10月主要出现在石榴园、小红枣园和水蜜桃园。对桔小实蝇在蒙自种群动态的调查,能够为下一步防治措施的制定做好准备。     

Neuroprotective Metabolites from Vietnamese Marine Derived Fungi of Aspergillus and Penicillium Genera

Low molecular weight secondary metabolites of marine fungi Aspergillus flocculosus, Aspergillus terreus and Penicillium sp. from Van Phong and Nha Trang Bays (Vietnam) were studied and a number of polyketides, bis-indole quinones and terpenoids were isolated. The structures of the isolated compounds were determined by 1D and 2D NMR and HR-ESI-MS techniques. Stereochemistry of some compounds was established based on ECD data. A chemical structure of asterriquinone F (6) was thoroughly described for the first time. Anthraquinone (13) was firstly obtained from a natural source. Neuroprotective influences of the isolated compounds against 6-OHDA, paraquat and rotenone toxicity were investigated. 4-Hydroxyscytalone (1), 4-hydroxy-6-dehydroxyscytalone (2) and demethylcitreoviranol (3) have shown significant increasing of paraquat- and rotenone-treated Neuro-2a cell viability and anti-ROS activity.     

农业生产基地项目环境影响评价要点分析及探讨

农业生产基地项目具有影响范围大、产品服务多元化等特点，涉及行业类别较多，其环境影响评价文件也相对复杂。环境影响评价报告编制单位应充分与建设方进行沟通，熟悉项目建设内容，挖掘项目可能的产生污染的环节和设施，明确环境影响范围和环境敏感点，并对项目损益进行两方面的分析。     

Analysis on Genetic Diversity and Phyletic Evolution of mtDNA Cytb Gene in Zhongdian Yak

In order to investigate the genetic diversity and the origin of evolutionary relationship of Zhongdian yak,we analyzed the complete sequence of 15 individuals Cytb gene,its sequence polymorphism was analyzed,and the phylogenetic tree was constructed.The results showed that the length of the nucleotide sequence were 1 140 bp,with nucleotide frequencies of 26.3%,31.8%,13.1% and 28.8% for T,A,G and C,respectively.Three haplotypes were identified of 15 individuals,with 3 polymorphic sites,including two conversions,one transversion,haplotype diversity was 0.2571 and nucleotide diversity was 0.00035.Phylogenetic analysis suggested that Zhongdian yak and Bos mutusc clustered firstly,then gathered with Bison bison,which indicated that there were high genetic similarity and closer genetic relationship,genetic similarity with other cattle genus was relatively low.Combining with the proof of molecular biology and paleontology,the result supported the point that Bos grunniens and Bos mutus were classified as an alone genus in Bovinae.     

思澜二级公路改建工程石拱桥加固利用

公路改建工程主要依托老路进行,旧桥加固利用是公路改建工程中重要的环节,对降低工程造价、缩短施工周期、缓解施工中的保通压力有其现实意义,针对思澜公路石拱桥加固利用,对老石拱桥加固技术和经济效益进行探讨。     

氮磷钾配施对苦瓜产量和品质的影响

采用田间试验的方法研究了氮磷钾配施对苦瓜产量和品质的影响。结果表明，氮肥、磷肥和钾肥以及适宜配合施用可显著提高苦瓜的产量。氮肥可提高苦瓜粗蛋白和氨基酸的含量，降低苦瓜维生素C和糖分含量，磷肥可提高粗蛋白和糖分含量，钾肥可以提高苦瓜维生素C、糖分和氨基酸含量，减缓施氮不合理带来的负面影响。充足的钾素供应和适宜的氮磷钾配比是保证苦瓜产量和品质的关键。     

无量山国家级自然保护区植被保护价值评价

对无量山国家级自然保护区的植被类型作群系级划分,进而从稀有性、特有性、生态重要性、脆弱性、易受干扰性、珍稀物种多度、物种多样性7个方面对保护区植被类型进行保护价值数量化评价,并对主要群系的保护价值作综合评价,位于保护价值前列的植物群落分别是:元江栲林,木果石栎林,壶斗石栎林,云南铁杉林和杜鹃、乌饭、八角矮林.     

昆明市西山区苹果的开花生物学特性与果实品质

对昆明市西山区大河果园主要苹果品种的开花生物学与果实品质进行调查研究,结果表明:花芽能顺利完成分化,乔纳金花芽分化要比红富士早,红富士的花芽坏死率为6.3%;各品种花期不一致,持续时间较长,约为20 d;各品种的果个不大,金冠果形指数为0.91~0.94、红富士为0.83~0.84;金冠苹果7月底时其成熟度和糖酸比都较低,应适当推迟采收期,红富士品种的成熟度和糖酸比在9月底达到较好的水平,可根据需要适时采收。