Pathogen Analysis of Bovine Respiratory Disease Complex

To investigate the pathogen of bovine respiratory disease complex (BRDC) of a dairy farm in Guangxi, a strain of Mycoplasma and a strain of gram-negative pathogenic bacterium were isolated and identified by the means of field surveys, clinic observation, pathological examination, isolation studies and so on.Treatments were taken according to drug sensitivity test results.The Mycoplasma strain, growing on PPLO medium, formed typical "fried egg" colonies.A 448 bp of oppF fragment was amplified by PCR from the strain and had 98.4% nucleotide identity with Mycoplasma bovis reference isolate PG5 of USA.The biochemical features of the gram-negative bacterial isolate were same with Serratia marcescens.The PCR amplified 16S rDNA of the gram-negative pathogenic bacterium strain was 1 400 bp.It shared 99.0% nucleotide identity with other Serratia marcescens reference strains obtained from GenBank.Animal experiment showed that the gram-negative pathogenic bacterium isolate could cause the mice to die.The drug sensitivity tests showed all isolates were sensitive to spectinomycin, azithromycin, amikacin, gentamicin and neomycin.It was effective to treat with dexamethasone and spectinomycin.Pathogen analysis and drug treatment showed that the BRDC was caused by Mycoplasma bovis and Serratia marcescens.     

基于旅游形象提升的河西走廊区域旅游营销策划

近年,河西走廊地带旅游业发展迅速,但比起其他省区,差距却十分明显,原因之一就是旅游形象定位、旅游营销策划和市场宣传上存在着弱点。基于此,在分析河西走廊现状旅游资源特征和旅游产品开发的基础之上,结合旅游市场调查和旅游地空间竞争分析,对地方文脉进行深入剖析,构建河西走廊区域旅游形象。最后,提出河西走廊区域旅游营销策划思路,...     

从实验室能力考核看试验的精确度控制

以本实验室参与的两次果蔬农残能力考核情况为例,通过对实验室两次考核结果的精确度控制的比较,对引起试验误差、导致试验质量失控的原因进行剖析,为农产品质量安全检测实验室的质量控制工作提供参考。     

Effect of antisense locked nucleic acid blocking translation initiation region of c-myc exon 2 on apoptosis of hepatocellular carcinoma cells

AIM: To observe the effect of antisense locked nucleic acid (anti-LNA) blocking the translation initiation region of c-myc exon 2 on the viability and apoptosis of hepatocellular carcinoma cells.METHODS: The anti-LNA that was complementary to the translation initiation region of c-myc exon 2 was designed, synthesized, and introduced into the HepG2 cells by cationic liposome-mediated transfection. The mRNA and protein levels of c-Myc in the cells were determined by RT-PCR and Western blot. The change of cell apoptosis was analyzed by flow cytometry, and the toxicity of anti-LNA to the cells was detected by MTT assay.RESULTS: Five days after transfection, the mRNA level of c-Myc in anti-LNA group was 0.335±0.016, and the protein level was 0.448±0.037, significantly lower than those in control group (both P<0.05). The ratio of apoptotic cells in anti-LNA group was 32%±6%, which was higher than that in control group (P<0.05).CONCLUSION: Antisense locked nucleic acid targeting at the translation initiation region of c-myc exon 2 shows strong inhibitory effects on the apoptosis of hepatocellular carcinoma cells.     

氨基酸和壳聚糖对烤烟生理特性及香味品质的影响

本文研究了叶面喷施氨基酸和壳聚糖对烤烟烟叶质体色素含量、酶活性的变异以及对致香物质含量的影响.结果表明:打顶后氨基酸处理烟叶叶绿素、类胡萝卜素、苯丙氨酸解氨酶(PAL)活性和脂氧合酶(LOX)活性较高,壳聚糖处理影响不显著;氨基酸处理的烟叶致香物质均比壳聚糖处理和CK高,尤其是对苯丙氨酸类、美拉德反应产物类影响显著,增幅分别达到20.9%、23.1%.壳聚糖对苯丙氨酸类致香物质有较大提高,增幅为17.6%.从整体上看,氨基酸处理调整了发育后期质体色素组分比例和相关酶类的活性,对烟叶香味品质的影响效果要好于壳聚糖.     

油桐育苗技术研究进展

油桐是我国重要的能源树种.为了加快我国油桐的良种化进程,推动油桐产业的进一步发展,从油桐的常规繁殖方法入手,介绍了我国油桐良种繁育的发展现状,分析了不同育苗技术的优缺点,对当前油桐种苗繁殖过程中存在的问题进行了探讨,并提出了相应的发展策略.     

尾巨桉( DH32-29)和尾叶桉(U6)-代林与二代林生长情况比较

对广西钦州市北区平吉紫胶林场尾巨桉( DH32 - 29)和尾叶桉(U6)人工林一代林与二代林(第一代萌芽林)蓄积生长情况进行对比分析,得出尾巨桉(U6)第二代林木单位面积年均蓄积生长明显低于一代林木,在相同立地条件下,对地力衰退影响也较大；而尾巨桉( DH32 - 29)第二代林木单位面积年均蓄积量与第一代林基本持平.说明尾巨桉人工林更适合在试验区进一步推广.     

圈养山羊添喂S25山羊浓缩料增重效果及效益分析

进行了圈养山羊添喂“山羊浓缩料”增重试验，结果显示：试验组羊群比对照组健康、少发病，平均每只增重分别比对照组Ⅰ和Ⅱ高38．1l％和131．37％，增重盈利效益分别比对照组Ⅰ和Ⅱ高181．71％和288．70％。     

百色市动物饲料中沙门氏菌分离鉴定检测报告

随机抽查百色市2009—2012年468份饲料进行沙门氏菌的检测,通过预增菌、选择性增菌和分离培养鉴定,检出阳性样品7份,阳性率1.5%。其中猪浓缩饲料样品检出5份,鸡配合饲料样品检出1份,自配大鸡混合饲料检出1份。检测发现猪浓缩饲料检出的几率较高,鸭、鱼、兔配合料未检出阳性样品,饲料中沙门氏菌污染主要来源于添加的动物蛋白质原料,添加动物蛋白质原料比例越高,检出的几率越大。     

百色市番茄病虫害综合防控主要技术措施

本文作者从农业防治、生物防治、物理诱控、化学防治等方面详见地归纳总结了百色市番茄病虫害综合防控主要技术措施。