功能高分子助剂G-100A调控农药对靶传递性能研究

随着统防统治技术的发展，将多种农药制剂结合喷雾助剂混配使用已成为主要施药方式之一，但由此也带来了不同企业制剂产品或配方助剂与喷雾助剂之间的桶混稳定性问题。本文将聚丙烯酸酯类微交联结构功能高分子助剂G-100A与脂肪醇聚氧乙烯醚类结构高分子助剂、三苯乙烯苯酚聚氧乙烯醚磷酸酯等小分子助剂复配后作为配方助剂，制备了40%苯醚甲环唑 ? 吡唑醚菌酯水乳剂，并在田间进行了防治水稻纹枯病的药效试验。结果表明:在推荐使用浓度下，所制备的40%苯醚甲环唑 ? 吡唑醚菌酯水乳剂药液对靶标的黏附力与沉积量显著提高，且具有良好的抗蒸发性能，在防治田间水稻纹枯病时，减量25%仍可达到对照药剂 40%苯醚甲环唑 ? 吡唑醚菌酯悬浮剂的防效。此外，G-100A还可作为喷雾助剂使用，其在药液中以特有的结构存在，可有效抑制水分蒸发、减少雾滴飘移，并可有效降低雾滴碰撞叶面时的界面能，防止雾滴弹跳。研究表明，G-100A有望作为农药减施的调控手段之一，在更多配方体系中得到应用。     

生态因子对芋根江蓠的生长及生化组分的影响

芋根江蓠（Gracilaria blodgettii）是江蓠属一种经济海藻，可作为琼胶工业原料和鲍鱼饲料，目前已经在中国南方池塘进行试验栽培。为了探讨适合其栽培的生态条件，文章分别测定了在不同温度（18 ℃、23 ℃、28 ℃、33 ℃和38 ℃）、不同盐度（13、18、23、28、33和38）和不同光照强度（3 000 lx、6 000 lx、9 000 lx和12 000 lx）的培养条件下，芋根江蓠藻体的相对生长速率（RGR）和生化组分的变化。结果表明，芋根江蓠适宜生长温度为23~38 ℃，最适温度为28~33 ℃；适宜盐度为18~38，最适盐度为23~33；适宜光照强度为6 000~9 000 lx，最适宜生长光照强度为9 000 lx。在最适的环境条件下，藻体具有较高的日相对生长速率（RGR），藻胆蛋白和可溶性蛋白（SP）的质量分数最高，过氧化物酶（POD）的活性及丙二醛（MDA）的质量摩尔浓度最低，受环境的胁迫最小。在生长状态、光合色素和抗氧化等方面，芋根江蓠具有适应高温和中、高盐度环境条件的特性。     

Model construction of rat coronary artery smooth muscle cell endoplasmic reticulum stress induced by thapsigargin

AIM: To investigate the primary culture method for coronary artery smooth muscle cells (CASMCs), and to establish the endoplasmic reticulum stress (ERS) model in CASMCs of SD rats. METHODS: CASMCs were cultured by tissue explant method. The morphological characteristics were observed under optical microscope. The marker proteins of CASMCs, including α-SMA and SM-MHC, were identified by immunofluorescence technique. The protein expression levels of BiP and CHOP, the marker molecules of ERS, were determined by Western blot. RESULTS: The spindle-shaped CASMCs climbed out from the edge of coronary artery tissues after 6 d, and formed the typical "hill and valley" growth pattern of CASMCs at 9~10 d. The result of immunofluorescence technique showed that α-SMA and SM-MHC were positively expressed. The results of Western blot showed that the protein expression of BiP and CHOP in TG (1 and 2 μmol/L) treatment groups was increased compared with control group. Compared with control group, the protein expression of BiP and CHOP was significantly increased after 1 μmol/L TG treatment for 24 and 48 h. CONCLUSION: CASMCs can be successfully cultured by tissue explant method. ERS model of CASMCs was established by 1 μmol/L TG treatment for 24 h.     

关于红草细长锥螺发生危害的专题报告及防治探讨

红草细长锥螺Dbeasgracile在汕头、潮州等地区大面积发生,对红绿草这种园林植物造成严重危害。文章综合分析探讨了红绿草细长锥螺分类地位、分布和寄主、形态特征、生物学特性、危害情况和防治方法。     

AutoCAD制图软件在汕头林业调查规划中的应用

从已有图纸资料的扫描输入,设置绘图环境,矢量化地形图,创建图层,用GPS数据绘制小班图,面积或路程求算,绘制图签、图例,文件保存格式,打印出图等方面阐述了AutoCAD在汕头市林业调查规划设计制图中的应用方法.认为用AutoCAD制图较Photoshop精度、效率高,并能较精确地进行面积及距离的求算.     

杂色鲍群体间杂交的杂种优势分析

杂色鲍（Haliotis diversicolor）是中国南方主要的鲍养殖种类,近年来“东优1号”杂色鲍因较高的成活率优势已在产业上得到越来越广泛的推广养殖.该研究采用3×3完全双列杂交的方式,对杂色鲍“东优1号”群体、杂色鲍古雷群体和杂色鲍越南群体3个群体自繁与杂交后代的养殖性能进行跟踪,并对其杂种优势进行评估.结果表明,3个群体自繁后代的生长速度和成活率上存在显著差异（P＜0.05）,同时3个组合的生长速度与成活率的双亲杂种优势率值均不高,且2个单亲杂种优势率值之间有显著差异（P＜0.05）.     

新特景观园林绿化树种象鼻树的引种初报

在汕头市中心城区引种象鼻树的结果表明：该种适宜引种区的气候和立地条件，生长发育良好，已能正常开花结实，且表现出较强的抗风、耐湿．抗寒和抗病虫能力等特性。     

2008年6月汕头一次特大暴雨天气成因分析

利用常规资料、ncep 2.5°×2.5°6 h再分析资料和卫星云图资料对2008年6月13日～14日汕头地区一次特大暴雨天气过程成因进行了综合分析。结果表明:高低层环流配置为暴雨提供了有利的大尺度环流条件;高低空急流的相互作用是此次暴雨产生的重要原因;中尺度对流云团是此次特大暴雨的直接影响系统;低空急流为暴雨的发生发展提供充沛的水汽和不稳定能量;高低空正负涡度柱和正负散度柱的耦合是产生暴雨的动力机制。     

黄瓜根际促生菌的促生效应与防病作用

对黄瓜植物根际促生菌(PGPR)菌株的分离筛选、分类鉴定以及人们对其促生防病作用的应用和防病机制的研究现状进行了综述,以期为新型PGPR制剂的进一步研制、开发和应用提供参考.     

Oxidative stress induces apoptosis in HepG2 cells

AIM: Direct exposure of cells to reactive oxygen species can induce apoptosis. In this study we investigate how oxidative stress induces cell death in HepG2 cells and characterize the molecular events involved.METHODS: Oxidative stress was created by exposing HepG2 cells to 2 mmol/L H2O2. Apoptosis was determined by analysis of DNA fragmentation by agarose gel electorphoresis. The mitochondrial membrane potential was analyzed using DePsipher fluorescent staining and the expression of cytochrome c in the cytosolic fraction was measured by Western blotting analysis. The caspase activity was detected using fluorometric assay kit by a fluorescence microplate reader.RESULTS: When HepG2 cells were treated with 2 mmol/L H2O2, the cells displayed DNA fragmentation, a typical feature of apoptosis, after 12 h. The mitochondrial membrane potential appeared different in two group of cells. H2O2-treated cells appeared green fluorescence as early as 4 h, which represents de-energized mitochondria, the untreated cells appeared red fluorescence, a feature of mitochondria with intact membrane potential. In treated cells, the expression of cytochrome c increased and accumulated in cytosolic fraction with treatment time, caspase-3 activity increased by 6.7-fold (P<0.01) at 8 h and caspase-9 activity increased by 3.6-fold (P<0.01) at 12 h, respectively, however, the activity of caspase-8 remained unchanged.CONCLUSION: These findings suggest that oxidative stress can induce apoptotic cell death in HepG2 cells, and the mechanism is related to mitochondrial pathway, which activates caspase-9 and-3, but not caspase-8.