Effect of RH-3 Fusion Hybrid on the Immune Function and Intestinal Microlora of Three Buff Chickens

The purpose of this experiment was to study the effect of RH-3 fusion hybrid of Bacillus subtilis and Lactobacillus rhamnosus on the immune function and intestinal microflora of three buff chickens.96 one-day-old chickens were randomly divided into 4 groups with 3 replicates per group and 8 chickens per replicate.Chickens in control group were drinking water that had not added the RH-3 fusion hybrid culture;Chickens in the experimental group were drinking water that supplemented with 0.5%, 1.0% and 1.5% RH-3 fusion hybrid culture, respectively.The whole experiment period was 42 days, including two phases of days 1 to 21 and days 22 to 42.The results showed that compared with the control group, the supplementation of RH-3 fusion hybrid increased the Newcastle disease HI antibody titer, SOD activity, lymphocyte transformation rate in serum and the immune organ index of three buff chickens at days 21 and 42, and the difference was significant of 0.5% level (P<0.05).Compared with the control group, the supplementation of RH-3 fusion hybrid reduced the number of Salmonella and E.coli and increased the number of the Bifidobacterium and Lactobacillus in jejunum, ileum and cecum of three buff chickens at days 21 and 42, and the difference was significant of 0.5% level (P<0.05).In summary, RH-3 fusion hybrid could improve the immune function and regulate the intestinal microflora of three buff chickens, promote the growth of beneficial bacteria and inhibit the growth of harmful bacteria, but the effects showed a downward trend with the increasing of the amount of RH-3 fusion hybrid.So the adding amount of RH-3 fusion hybrid in the drinking water of three buff chickens was suggested as 0.5% under this experimental condition.     

RH-3融合子对三黄鸡免疫功能及肠道菌群的影响

试验旨在研究枯草芽孢杆菌和鼠李糖乳杆菌原生质体融合子(RH-3融合子)对三黄鸡免疫功能及肠道菌群的影响。选用96只1日龄三黄鸡,随机分为4组,每组3个重复,每个重复8只雏鸡;对照组饮水为不添加RH-3融合子培养物的清水;试验组分别在三黄鸡的饮水中添加0.5%、1.0%和1.5% RH-3融合子培养物。试验期42 d,分1~21和22~42日龄两个阶段。结果表明,与对照组相比,RH-3融合子可提高21和42日龄三黄鸡血清新城疫HI抗体效价、SOD活性和淋巴细胞转化率,增加免疫器官指数,其中0.5%添加水平差异显著(P<0.05);与对照组相比,RH-3融合子可降低21和42日龄三黄鸡空肠、回肠和盲肠内容物中的沙门氏菌和大肠埃希菌的菌群数量,增加双歧杆菌和乳酸菌的菌群数量,其中0.5%添加水平差异显著(P<0.05)。综上所述,RH-3融合子可改善三黄鸡免疫功能,调节肠道微生物区系,促进有益菌的生长,抑制有害菌的生长,且随着RH-3融合子添加水平的增加,效果均呈现下降的趋势。因此,在本试验条件下,建议三黄鸡饮水中RH-3融合子添加水平为0.5%。