稻虱缨小蜂耐热品系的生物和生态学特征

稻虱缨小蜂Anagrus nilaparvatae Pang et Wang是控制稻飞虱种群暴发的主要寄生性天敌之一，高温是制约其种群动态的重要因子。为了解35℃高温驯化获得的稻虱缨小蜂耐热品系（NR品系）的生防潜力，对不同温度下该品系的发育历期、有效积温、寄主卵选择、产卵量以及极端高温胁迫参数进行了测定。结果发现，与未经高温驯化的稻虱缨小蜂品系（HZ品系）相比，20℃时，NR品系雄蜂的发育速率显著高于HZ品系，这一差异主要表现在卵-幼虫中期和蛹期的发育时间明显缩短。NR品系在高温下能维持正常的产卵能力，35℃下NR品系产卵量仍能达到18.80粒/雌。NR品系有更强的耐极端温度的能力，50℃下NR品系雄蜂的热击倒时间为15.19 min，比HZ品系雄蜂长3.03 min。补充不同营养对NR品系的产卵量存在影响，35℃条件下补充10%蔗糖既能延长其寿命，也能提高产卵量。以上研究结果表明高温驯化得到的稻虱缨小蜂品系在35℃高温环境中能维持正常的生长发育和寄生能力，具备适应稻田夏季高温环境的潜力。     

基于健康安全的棕地再开发决策模型构建及模拟研究——以广东省F市为例

为提高棕地再开发决策的科学性与安全性，以“建设用地准入机制”和“土壤环境质量标准”等为指导思想和标准依据，结合环境、经济、社会和地块特征等因素，构建棕地再开发决策指标体系及决策模型，运用模糊综合法对目标地块拟开发方向进行决策模拟。以广东省F市土壤环境质量调查和土地利用规划数据为基础，结合“三旧改造”规划方案，测算土壤污染程度，采用ArcGIS进行可视化安全评估，对目标地块再开发方向进行决策模拟，并以变动权重组合进行验证。结果表明： 37%的棕地再开发方向需要修正，在各影响因子中，环境因素权重最大，地块特征其次，社会和经济因素最小。研究表明，模拟决策模型确定的土地分级分类风险管控措施对棕地再开发决策具有较好的指导作用。     

以竹代塑新产品竹微丝复合包装材料的制备及其性能

为减少塑料制品的使用,缓解其对全球环境的污染,以质量分数为90%的竹微丝、2%的聚乙烯、8%的甘油制备竹微丝复合材料,参照传统竹编织技艺,分别采用纯纵向编织法和纵横向编织法等制备了竹微丝复合包装材料,并对其性能进行分析。结果表明:1)与通过传统竹篾工匠手工制备的竹微丝相比,由大型智能化竹拉丝设备制备的竹微丝边缘更整齐;2)与竹微丝单方向编织技术相比,采用纵横向交叉编织技术的竹微丝复合包装材料的抗拉性能更好;3)竹微丝复合包装材料的拉伸强度远大于PE、PVC等传统塑料包装材料。     

拟穴青蟹Cactus基因的cDNA克隆、序列及生物学功能

为获取拟穴青蟹Cactus基因cDNA全长、分析基本生物学信息,并初步探索其在病原物刺激下的免疫反应,实验采用RACE技术获得了拟穴青蟹Cactus(SpCactus)基因的cDNA全长序列,其cDNA全长为2035 bp,开放阅读框(ORF)1311 bp,编码436个氨基酸,分子量为46.01 ku。对蛋白理化性质进行预测发现,SpCactus为亲水性蛋白,等电点pI为4.91。经预测,SpCactus与其他物种的IκB蛋白具有相似的功能结构域。同源性比对结果显示,SpCactus与凡纳滨对虾和中国明对虾的Cactus蛋白同源性均高达62%,相似度为73%。系统进化树分析显示,SpCactus与甲壳动物聚为一支,与无脊椎动物聚为一大支。实时荧光定量PCR(RT-PCR)检测发现,SpCactus基因在拟穴青蟹不同组织中均有表达,肌肉中的表达量最高,其次为心脏、眼柄、血液和鳃,肝胰腺中的表达量最低。LPS和金黄色葡萄球菌刺激均能显著诱导SpCactus基因的表达。本实验成功扩增了SpCactus基因全长,并进行了生物信息学分析、理化性质预测,初步探讨了其生物学功能,为进一步研究其在免疫反应中的生物学功能提供参考依据。     

Occurrence and Effects of the Rhizocephalan Parasite,Polyascus gregarius,in the Chinese Mitten Crab,Eriocheir sinensis,Cultured in a Freshwater Pond,China

An epizootic outbreak of the parasitic barnacle, Polyascus gregarius, was reported for the first time in cultured commercial mitten crab, Eriocheir sinensis. Observations were made in a freshwater culture pond in Shenjia Dang, Zhejiang province, China, from June to October 2007. The surface morphology and appearance of the interna, externa, or scar of P. gregarius were recorded in 1464 crabs, including 871 males and 593 females. Overall prevalence of infection was 37.8 and 31.7% in male and female crabs, respectively. The monthly variation of infection was mainly dependent on the mortality of the host, with the highest prevalence (58.0% of males and 51.3% of females infected) in June, and the lowest (13.4% of males and 6.3% of females) in October. A host with multiple externae or scars was encountered frequently, with the maximum of 28 externae or 19 scars on a single crab. Being restricted in the culture pond, the hosts could not escape and return to the brackish water of the estuary; therefore, the externae were never exposed to male cyprids and remained unfertilized. As a result, all the externae degenerated and dropped off finally after 2–3 mo. This parasite caused castration, anecdysis, and increased mortality in the host, and therefore, had both direct and indirect effects on the commercial culture of E. sinensis. Infected males were castrated and displayed morphological modifications of secondary sexual characters. The size of infected crabs was significantly smaller than that of uninfected crabs because the molting was reduced by infection. After all the externae dropped off, the scarred host crabs finally died. A high mortality (possibly >50%) due to parasitism was observed in this study.     

Growth,Survivorship, Air‐bubble Disease,and Attachment of Feeble Juvenile Seahorses,Hippocampus kuda (Bleeker, 1852)

Seahorse aquaculture has been a focus for meeting the demand of traditional medicine and aquarium. Feeble (poor quality) juveniles are commonly found in the commercial seahorse culture. In this study, we compared the growth and survival performances of the feeble and healthy common seahorses Hippocampus kuda. The results show that the feeble juveniles had high growth and survival at the temperature of 27–29 C and salinity of 26–28‰ with frequent feeding (≥three times a day) during first 5 wk. Through a 12‐wk investigation, we found that the air‐bubble disease could significantly affect the specific growth rate and survival of the feeble juveniles. There was a positive and significant correlation between the substrate‐attachment rate and survival rate in the feeble juveniles, and substrate‐attachment rate may be used to assess the quality of the feeble populations in the seahorse H. kuda.     

Plant establishment on a green roof under extreme hot and dry conditions: The importance of leaf succulence in plant selection

Plant selection for extensive green roofs has largely been based on cool, temperate climate research. However, as green roof implementation in hotter and drier climates increases, there is a need to evaluate plant performance under these climatic conditions. Succulents have been shown to be successful in hot and dry green roofs, although survival differs between species and the role of leaf succulence in survival has not been fully explored. For non-succulent plants, habitats with conditions similar to green roofs (‘habitat templates’) have been used to select plants, although few studies have discussed the performance of these selections under green roof conditions. Therefore, we evaluated establishment of 32 plant species on an unirrigated extensive (125 mm deep) green roof in Melbourne, Australia over a 42 week period (from winter through summer into autumn). Plants were selected on the basis of life-form, succulence, appropriate habitat templates and/or successful use on green roofs internationally. Climatic conditions during the experiment were often extreme, with evaporation regularly exceeding rainfall and a hot and dry summer (mean maximum air temperature 35 °C and 80.6 mm total rainfall), leading to roof temperatures of 65 °C. After 42 weeks, only succulent plants remained alive and only three of the succulent species had 100% survival. Survival was positively related to the degree of leaf succulence (g H₂O leaf area cm⁻¹) making this a useful trait for plant selection for unirrigated green roofs in hot, dry climates. The failure of most species, despite being chosen from appropriate habitats, demonstrates the need to evaluate potential plants on green roofs under extreme climatic conditions. Supplementary irrigation may be essential to sustain non-succulent species during extreme weather in hot and dry climates.     

近江牡蛎（Crassostrea hongkongensis）家系早期发育生长比较

选取数量性状有较大差异的6个近江牡蛎（Crassostrea hongkongensis）作为亲本，成功建赢了9个半同胞家系，并对各个家系的受精、孵化、成活和早期生长的情况进行比较。结果显示，各家系子代的受精率、9孵化率和成活率均受亲本成熟度的影响；各家系的早期生长中，家系CH1、CH4和CH7的生长率大于其他家系；近江牡蛎幼虫的早期生长阶段受母本效应影响明显。