全文获取类型
收费全文 | 745篇 |
免费 | 9篇 |
国内免费 | 7篇 |
专业分类
林业 | 15篇 |
农学 | 7篇 |
20篇 | |
综合类 | 42篇 |
农作物 | 13篇 |
水产渔业 | 4篇 |
畜牧兽医 | 19篇 |
园艺 | 635篇 |
植物保护 | 6篇 |
出版年
2023年 | 3篇 |
2022年 | 2篇 |
2021年 | 4篇 |
2020年 | 8篇 |
2019年 | 10篇 |
2018年 | 9篇 |
2017年 | 11篇 |
2016年 | 20篇 |
2015年 | 31篇 |
2014年 | 35篇 |
2013年 | 55篇 |
2012年 | 49篇 |
2011年 | 68篇 |
2010年 | 51篇 |
2009年 | 45篇 |
2008年 | 46篇 |
2007年 | 55篇 |
2006年 | 56篇 |
2005年 | 49篇 |
2004年 | 47篇 |
2003年 | 36篇 |
2002年 | 32篇 |
2001年 | 24篇 |
2000年 | 15篇 |
排序方式: 共有761条查询结果,搜索用时 140 毫秒
1.
LUO Li-hui FU Xiao-ying ZHENG Yang-xi LIANG Wei-jie ZHI Xi-mei DENG Hai-ou ZHANG Wei-jie WANG Rui-xue WU Wen 《园艺学报》2020,36(4):598-605
AIM To investigate whether pyroptosis contributes to the inflammation and injury in mouse embryonic osteoblastic cell line MC3T3-E1 induced by high glucose (HG; 45 mmol/L glucose). METHODS The cell viability was measured by CCK-8 assay. The protein expression levels of nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) and caspase-1 (CASP1) were determined by Western blot. The secretion levels of interleukin-18 (IL-18) and IL-1β were measured by ELISA. The intracellular level of reactive oxygen species (ROS) was detected by 2',7'-dichlorodihydrofluorescein diacetate staining followed by photofluorography. Mitochondrial membrane potential (MMP) was examined by rhodamine 123 staining followed by photofluorography. The alkaline phosphatase (ALP) activity was determined using the ALP kit, and the number of mineralized nodules was detected by alizarin red S staining. RESULTS After the MC3T3-E1 osteoblasts were treated with HG for 24 h, the protein expression levels of NLRP3 and CASP1, and the secretion levels of IL-18 and IL-1β were significantly increased. The decrease in cell viability, and the increases in ROS generation and MMP loss were also observed. Moreover, the differentiation and mineralization of MC3T3-E1 osteoblasts were inhibited, evidenced by decreases in both ALP activity and mineralized nodule number. Knockdown of CASP1 by siRNA attenuated the HG-induced osteoblast inflammation and injury mentioned above. CONCLUSION Pyroptosis mediates HG-induced inflammation and injury in MC3T3-E1 osteoblasts. 相似文献
2.
AIM: To investigate the inhibitory effect of microRNA-145 (miR-145) on epithelial-mesenchymal transition (EMT) in renal cancer A-498 cells. METHODS: The A-498 cells were transfected with miR-145 mimics (M145) and mimic negative control(MNC), which served as M145 group and MNC group, respectively. Mock control (MC) group was set up using untreated A-498 cells. The expression level of miR-145 in each group was detected by RT-qPCR. Transwell assay was used to detect the invasion ability of the cells. The protein expression of vimentin, E-cadherin and ADAM28 was determined by Western blot. Bioinformatic method was used to predict the target genes of miR-145. Antagonistic effect of ADAM28 over-expression on the inhibition of EMT by miR-145 was detected by Western blot. The relationship between miR-145 and ADAM28 was analyzed by dual-luciferase reporter assay. RESULTS: The expression level of miR-145 in M145 group was significantly up-regulated than that in MC group (P<0.05). The number of invasive cells in M145 group was 12.78±3.37, which was significantly lower than that in MC group (P<0.05). ADAM28 may be the target gene of miR-145. Compared with MC group, the protein expression of vimentin and ADAM28 in M145 group was significantly decreased (P<0.05), while the protein expression of E-cadherin was significantly increased (P<0.05).After ADAM28 over-expression, the protein expression of vimentin in the A-498 cells of M145 group was significantly increased (P<0.05), and the protein expression of E-cadherin was significantly decreased (P<0.05). The results of dual-lucife-irasei reporter assay showed that ADAM28 was a downstream target gene of miR-145. CONCLUSION: miR-145 may inhibit the expression of EMT-related proteins through the downstream target gene ADAM28 and inhibit the EMT process of renal cancer A-498 cells. 相似文献
3.
《Fitoterapia》2014
Four new compounds, microminutin B (1), microminutin C (2), micromarinate (3), and secomicromelin (4) as well as 17 known compounds were isolated from the fruits of Micromelum falcatum. All compounds were evaluated for antifungal activity against Pythium insidiosum using disc diffusion assay. P. insidiosum is a fungus-like microorganism for which antifungal agents now available are not effective. The results show that four compounds including secomicromelin (4), 7-methoxy-8-(4′-methyl-3′-furanyl)coumarin (10), micromarin B (17), and isomicromelin (19) could inhibit the mycelia growth of P. insidiosum. 相似文献
4.
AIM: To explore whether IL-1β inhibits the oligodendrocyte precursor cell (OPCs) differentiation and affects axonal myelination. METHODS: One-day-old SD rats were randomly divided into control group and LPS group (48 rats in each group). The rats in LPS group were intraperitoneally injected with 1 mg/kg LPS. The rats in control group were injected with an equal volume of PBS. The rats in each group were further divided into 3 h, 24 h, 3 d, 7 d, 14 d and 28 d subgroups after injection. The expression of IL-1β and IL-1R1 in the rat corpus callosum at 3 h, 24 h, 3 d, 7 d was determined by double immunofluorescence and Western blotting. The myelin basic protein(MBP) expression in the rat corpus callosum at 14 d, 28 d after injection was also measured. In vitro, primary OPCs culture was performed and divided into control group, 30 μg/L IL-1β group, 30 μg/L IL-1β+IL-1Ra group and 30 μg/L IL-1Ra group. The expression of MBP in the OPCs induced differentiation for 3 d was observed by double immunofluorescence and Western blotting. RESULTS: The expression of IL-1β and IL-1R1 in the rat corpus callosum at 3 h, 24 h, 3 d, 7 d after LPS injection was obviously increased and the expression of MBP in the rat corpus callosum at 14 d, 28 d in LPS group was obviously decreased compared with control group in vivo. The level of MBP was significantly decreased after IL-1β treatment for 3 d in vitro. However, IL-1Ra (IL-1R inhibitor) reversed the down-regulation of MBP expression. IL-1β inhibited the expression of p-ERK, ERK over-expression reversed the down-regulation of MBP expression compared with IL-1β group. CONCLUSION: IL-1β inhibits the differentiation of OPCs, which may be involved in ERK pathways, thus leading to axonal hypomyelination in the corpus callosum of septic neonatal rats. 相似文献
5.
AIM: To investigate the role of mesenchymal stem cell-induced regulatory dendritic cells (MSC-DCregs) in mouse acute graft-versus-host disease(aGVHD) model. METHODS: Bone marrow cells from BALB/c (H-2d) mice were isolated and were induced to differentiate into DCs. The DCs were selected by flow cytometry, and after 10 d co-culture with MSCs, they were induced to be MSC-DCregs. Male 8-week-old C57BL/6 (H-2b) mice were used as donor mice. The female 8-week-old BALB/c (H-2d) mice, who had received 100 cm source-skin distance, 30 cm×30 cm radiation field, 700 cGy total body irradiation (TBI) pretreatment were used as recipient mice. The recipients were divided into 5 groups: control group, TBI group (injected with medium only), bone marrow transplantation group (injected with 1×107 bone marrow cells), aGVHD group (injected with 1×107 bone marrow cells and 1×107 spleen cells), and MSC-DCregs group (injected with 1×107 bone marrow cells, 1×107 spleen cells and 1×106 MSC-DCregs). The white blood cell count, recipients' chimerism, clinical evaluation of aGVHD, survival analysis and pathological changes were determined. RESULTS: Hematopoieic recovery was seen at 10 d after transplantation. The recipients' chimerism was parallel to the donors' at 30 d. The median survival time of the mice in aGVHD group and MSC-DCregs group was 27 d and 33 d, and the survival rates at 30 d were 20% and 100% (P<0.01), respectively. The clinical scores of the mice in MSC-DCregs group were lower than those in aGVHD group (P<0.01). Moreover, the pathological changes in the skin and liver of the mice in MSC-DCregs group were less serious than those in aGVHD group. CONCLUSION: The MSC-DCregs induce an aGVHD tolerance in vivo, and further research of its mechanism is still in great necessary. 相似文献
6.
HU Bin ZENG Bing-hui HU Yue-lin ZHAO Qiang JING Xiang-yi ZHANG Yong-ling WANG Yi-ming 《园艺学报》2015,31(7):1237-1241
AIM: To investigate the underlying genetic changes of a Chinese patient with infantile malignant osteopetrosis (IMO). IMO is a monogenic disease, mostly caused by mutations of TCIRG1 and CLCN7 genes. The former is believed a homozygous gene and only cause the disease in homozygous or compound heterozygous status. However, it has been reported that heterozygous mutations also cause the disease in 6 non-Chinese cases. METHODS: Genomic DNA was extracted from peripheral blood of the patient and his parents. All exons and splice sites of TCIRG1 and CLCN7 genes were amplified by PCR followed by Sanger sequencing. Mutation detection in the 2 genes was also investigated in the parents. Haplotypes were constructed by variations obtained in mutation detection and microsatillites flanking TCIRG1 gene in the family by Cyrillic. Chromosomal microarray analysis (CMA) was performed to detect copy number variations (CNV) of the patient and his mother. RESULTS: A novel mutation c.449_452delAGAG (p.Gln149Glnfs16) was detected in the patient. This mutation truncated 666 amino acids at the C terminal of the V-ATPase 116 kD isoform a3 protein. It wiped out the entire ATPase V0 complex and was predicted to result in total loss of protein function. This mutation was also detected in the patient's father. No pathogenic mutation was detected in CLCN7 gene. CMA did not reveal any CNV involving TCIRG1 or CLCN7 gene. CONCLUSION: We reported a novel heterozygous mutation of TCIRG1 gene causing IMO. This represents the first IMO case in China caused by heterozygous TCIRG1 gene mutation. 相似文献
7.
XU Jing-ting CAO Ya-lin ZHENG Wen-hui ZHANG Ya-xing WANG Ling WANG Ting-huai 《园艺学报》2015,31(6):1008-1013
AIM: To investigate the effect of Jia-jian-yi-yin decoction on endothelium-dysfunction in ovariectomized rats. METHODS: The ovariectomized rats were treated with Jia-jian-yi-yin decoction or turbid liquid of estradiol valerate for 8 weeks. The vascular ring tension was measured. Scanning electron microscopy and Western blotting were applied to assess the function of endothelium-dependent dilation, aortic endothelial morphology and the expression of endothelial lipase. The pathologic sections were prepared to observe the effect of Jia-jian-yi-yin decoction on the uterus. RESULTS: In ovariectomized rats, the decrease in endothelium-dependent relaxation to acetylcholine (ACh) was reversed to normal level, the endothelial morphology returned to normal without lipid accumulation and the endothelial lipase expression was decreased by Jia-jian-yi-yin decoction. Furthermore, no obvious change of the wet weight of uterine between the ovariectomized rats with or without Jia-jian-yi-yin decoction treatment was observed. CONCLUSION: Jia-jian-yi-yin decoction may have protective effects on endothelium-dependent vasodilation and aortic endothelial morphology in estrogen-deficient animals. 相似文献
8.
LIANG Meng-ya TANG Zhi-xian CHEN Guang-xian RONG Jian DAI Gang WU Zhong-kai 《园艺学报》2015,31(5):823-827
AIM: To observe the expression of hypoxia-inducible factor 1 (HIF-1) and neuroglobin (NGB) in piglet cortex during deep hypothermic circulatory arrest. METHODS: Wuzhishan piglets were randomly assigned to cardiopulmonary bypass group (CPB group), 40 min of circulatory arrest (CA) at 18 ℃ without cerebral perfusion (DHCA group) or with selective antegrade cerebral perfusion (SACP group). After 180 min of reperfusion, cortical tissue was harvested for determining HIF-1α and NGB expression by HE staining, Western blot and real-time PCR. RESULTS: Severer cerebral injury was observed in DHCA group than that in SACP group. After 180 min of reperfusion, HIF-1α protein and mRNA levels were significantly higher in DHCA group than those in CPB group (P<0.05). Accordingly, SACP animal had higher levels of HIF-1α protein and mRNA than those in DHCA group (P<0.05). Simultaneously, higher NGB protein and mRNA levels were found in DHCA group than those in CPB group after 180 min of reperfusion (P<0.05). The SACP animal had higher levels of NGB protein and mRNA than those in DHCA group (P<0.05). CONCLUSION: Up-regulation of HIF-1 and NGB are involved in the mechanism against cerebral injury resulting from DHCA in the cortex and possibly a part of cerebral protective effect of SACP. 相似文献
9.
RAO Fang XUE Yu-mei DENG Chun-yu YU Xi-yong XIAO Ding-zhang CHEN Shao-xian LIN Qiu-xiong YANG Hui KUANG Su-juan LIU Xiao-ying ZHU Jie-ning WU Shu-lin 《园艺学报》2015,31(11):1986-1991
AIM: To investigate whether the association of connexin 43(Cx43) and L-type calcium channel involved in the pathogenesis of atrial fibrillation (AF). METHODS: The biochemical assays and whole-cell patch-clamp technique were used to study the expression of Cx43 in human atrial tissue. The co-localization of Cx43 and L -type calcium channel, and the regulation of L-type calcium current in atrial myocytes were investigated. RESULTS: The expression of Cx43 at mRNA and protein levels was decreased in human atrial tissues of AF patients. In cultured atrium-derived myocytes (HL-1 cells), knockdown of Cx43 significantly inhibited the mRNA expression of L-type calcium channel α1c subunit, as well as L-type calcium current. Co-localization of Cx43 with L-type calcium channel α1c subunit in mouse atrial myocytes was observed. CONCLUSION: The decrease in Cx43 is involved in the pathogenesis of AF, probably through reducing the L-type calcium current in atrial myoctyes by co-localization with L-type calcium channel, thus representing the potential pathogenesis in atrial fibrillation. 相似文献
10.
西藏拉萨地区5000a来古气候古环境变迁 总被引:1,自引:1,他引:0
根据西藏拉萨桑达第四系剖面的研究,采用植硅体、分子化石等气候待用指标,并配以TL测年,探讨了拉萨地区5000a来气候及环境变迁.结果表明:1)在5.889Ka.BP-2.3 Ka.BP,植硅体形态类型为齿形-平滑棒型、石屑型、网脊块状,反映的植被景观为森林-草原景观,气候温凉;C27+C29/C31+C33值>1,木本... 相似文献