高山马铃薯脱毒苗DNA甲基化的MSAP分析

为了解脱毒对高山马铃薯试管苗DNA甲基化水平的影响,探究DNA 甲基化在高山马铃薯脱毒后的作用,结合毛细管电泳检测技术对怀玉山高山马铃薯脱毒苗的基因组DNA甲基化进行MSAP分析。结果表明,怀玉山高山马铃薯常温继代带毒苗甲基化水平较高,经过玻璃化法超低温保存处理但未能脱毒时其甲基化水平有所下降,但甲基化仍处于较高水平,而常规茎尖培养脱毒和玻璃化法超低温疗法脱毒可明显降低试管苗的甲基化水平,且玻璃化法超低温疗法脱毒苗的甲基化水平更低。在怀玉山高山马铃薯超低温保存(未能脱毒)、茎尖常规培养脱毒和超低温疗法脱毒3个处理方式中,去甲基化模式和甲基化模式并存,但均以去甲基化模式为主要趋势,且在去甲基化模式的强弱依次为超低温疗法脱毒>茎尖常规培养脱毒>超低温保存(未脱毒)。本研究结果为怀玉山高山马铃薯脱毒苗的种质保存和规模化生产提供了一定的理论依据。     

The Effect of Trehalose Supplementation of INRA-82 Extender on Quality and Fertility of Cooled and Frozen-Thawed Stallion Spermatozoa

Stallion semen cryopreservation is often associated with poor post-thaw sperm quality. Sugars act as nonpermeating cryoprotectants. The aim of the present study was to evaluate the cryoprotective effect of trehalose on stallion sperm quality and field fertility rates subjected to cooling and freeze–thaw process. Semen samples were collected from six Arabian stallions, divided into five different treatments in a final concentration of 100 × 10⁶ sperm/mL by using INRA-82 extender containing 0, 25, 50, 100, and 200 mM of trehalose then subjected to both cold storage and cryopreservation. Sperm motility, acrosome, plasmatic membrane, and DNA integrity were analyzed, and 57 mares were used to evaluate the field fertility of chilled and frozen-thawed semen. Results showed that the extender containing 100 mM trehalose only increased the functional acrosomal, plasma membrane, and DNA integrities. The inclusion of 50 mM trehalose in semen extender resulted in significantly (P < .05) increased post-thaw total motility compared to the control group, and chilled semen achieved higher pregnancy rates compared to the frozen-thawed one. Pregnancy rate of mares inseminated with frozen-thawed semen (P < .05; 46.15% vs. 36.36%, respectively) was lower than those inseminated with chilled semen (76.47% vs. 68.75%, respectively) but higher than control. In conclusion, addition of 50 mM trehalose yielded the highest quality stallion semen after cooling and post-thawing in terms of motility, integrities of acrosome, membrane, and DNA as well as improved field fertility.     

多变时滞Volterra型动力系统的稳定性

探讨了一类多变时滞积分微分动力系统,并通过不动点方法给出了该系统零解渐近稳定的充要条件.在构造算子时,根据动力系统时滞特点,分别引入对应的连续函数h_i(s),i=1,2,…,n,然后利用这n个函数来构造算子,最后再利用Banach不动点方法来研究该动力系统的稳定性.     

外源水杨酸对水稻幼苗抗冷性的影响

以1.0、1.5、2.0、2.5、3.0、3.5 mmol/L水杨酸和蒸馏水处理一叶一心期的水稻幼苗,然后分别置于5℃和自然条件下生长,研究了水杨酸对水稻幼苗抗冷性的影响。结果表明:在5℃低温胁迫下,经1.5 mmol/L水杨酸处理的水稻幼苗的脯氨酸含量、叶绿素含量和SOD活性均高于仅用蒸馏水处理的对照,而丙二醛含量则低于对照,表明一定浓度的水杨酸可增强水稻幼苗的抗冷性,但外源水杨酸处理对冷胁迫下水稻幼苗生长存在"低促高抑"的效应。     

植物生长调节剂对甘蓝型油菜愈伤组织诱导和生长的影响

利用植物组织培养方法,探讨甘蓝型油菜种子的最佳消毒方式以及叶片和茎段在附加不同质量浓度KT,6-BA,NAA,2,4-D的MS培养基中愈伤组织诱导的影响。结果表明,对于不同品种甘蓝型油菜种子采用2种消毒方式进行培养,污染率都较低。B1,B10,A5品种的第Ⅱ种消毒方式(75%酒精2 min+0.1%HgCl220 min)较好;B4品种的2种消毒方式(75%酒精2 min+0.1%HgCl2 20 min和75%酒精3 min+0.1%HgCl230 min)差别不大。B1,B4,B10,A5品种的甘蓝型油菜叶片和茎段愈伤组织诱导的结果表明,不同激素组合对叶片和茎段愈伤组织诱导及生长都有很大的影响,其中,MS+6-BA 2 mg/L+NAA 0.5 mg/L培养基有利于愈伤组织诱导及生长。     

福建武夷山保护区蓼属植物种质资源调查及园林应用分析

通过对福建武夷山蓼属植物种质资源的初步调查,结果发现武夷山自然保护区的蓼属植物包括23种,其中园林应用价值较高的有7种,分别是长鬃蓼、从枝蓼、尼泊尔蓼、细叶蓼、愉悦蓼、柳叶蓼、红蓼.结合景观生态学的原理,对这7种蓼属植物在园林绿地中的应用进行了初步的分析和探讨.     

香果树带芽茎段不定芽高频增殖的优化

以香果树(Emmenopterys henryi Oliv.)带芽茎段为外植体,以MS为基本培养基,采用正交试验和单因子试验方法研究了不同因素对带芽茎段不定芽增殖的影响。结果表明:香果树带芽茎段不定芽增殖的最佳培养基为MS+KT2.0mg/L+6-BA2.0mg/L+NAA0.1mg/L,最佳蔗糖和琼脂浓度分别为40和7.5g/L,最佳pH值6、培养温度25℃,培养条件为光培养。证明利用组织培养技术能够实现香果树带芽茎段不定芽的高频增殖,为珍稀濒危植物香果树的快速繁殖提供了一条简单.有效的途径。     

植物生理学实验教学中创造性思维能力的培养

针对师范院校植物生理学实验教学中存在的问题,对教学内容、教学结构以及考核方法等进行了改革.实践证明,该方法对提高学生认识、分析、解决问题的能力以及创新能力的培养是行之有效的,并提出了改进方法.     

Appraisal of growth curve in Sirohi goat using non-linear growth curve models

Tropical Animal Health and Production - In the present study, the appropriateness of five non-linear mixed growth curve models was studied to describe body weight growth of Sirohi male and female...     

白耳黄鸡生产性能和肉质性状的研究

对白耳黄鸡生长性能、屠宰性能、常规肉质、胸腿肌化学成分、肌肉组织学特性、胸肌氨基酸和脂肪酸含量等指标进行了研究。白耳黄鸡公母鸡的屠宰率、胸肌率、腿肌率、蛋白质含量、氨基酸总量、腹脂率、人体必需氨基酸含量、必需脂肪酸含量等分别为89.23/90.18%,16.65/18.09%,22.37/21.91%,23.64/25.30%,206.29/218.8mg/g,1.64/2.39%,92.78/96.75mg/g,30.69/29.52%。结果表明,白耳黄鸡的各指标都表现优良,其具有屠宰性能好,肉质优良,必需氨基酸和脂肪酸含量高,腹脂率低等特点,是一个值得推广的地方优良品种。