内2优6号生育特性及高产栽培技术模式研究

内2优6号全生育期146d,适于单季稻种植,分蘖力中等,株型较大,每穗约200粒,粒重高,千粒重30.5g,抗倒性较强。但叶片较长较宽。高产栽培的关键,培育壮苗,扩行稀植,控制中后期氮肥用量,增施钾肥,浅湿干灌溉,促进根系生长,提高根系活力和结实率。     

芋疫病空间分布格局及其抽样技术研究

为了进一步提高对芋疫病预测预报,科学指导生产上的防治,应用最小二乘法、频次分布、聚集度指标、m^*-m回归分析和Taylor幂法则等对病株的空间分布型进行了分析。结果表明:当田间芋疫病病株率在0.427~0.513时,病株田间分布属聚集分布;当田间芋疫病病株率在0.720~0.820时,病株田间分布属均匀分布。此外其病株空间分布的基本成分是个体群,病株个体间相互吸引,病株在大田中存在明显的发病中心,且病株个体的空间格局随着病株密度的提高越趋均匀分。在此基础上,提出了Iwao最适理论抽样模型N=232.3783/m-87.9438,并建立序贯抽样模型T₀(N)=0.3689N±1.7177$\sqrt{N}$,即:调查株数N时,若累计病株率超过上界可定为防治对象田,若累计病株率未达到下界时,可定为不防治田,若累计病株率在上下界之间,则应继续调查,直到最大样本数m₀=0.3689时,也即病株率15%,所需抽样数542株止。     

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 ‘台绿1 号’是分别以日本青花菜品种‘绿雄90’和‘Br60’为原始材料经连续多代自交选 育的自交不亲和系‘B19-10-1-2-1’和‘Br60-2-2-1-2’为母本和父本杂交育成的青花菜新品种。生长势较 强，植株半直立，叶片长椭圆形，叶色深绿，蜡粉中等。花球紧实，高圆形且圆整，蕾粒中细，单球质 量0.76 kg，产量约23 000 kg · hm^-2。中晚熟，从定植到采收约95 d，保鲜和速冻加工兼用。适合长江流域秋、冬季栽培。     

斯蒂齿斑摇蚊的再描述(双翅目:摇蚊科)

对摇蚊科（Chironomidea）斑摇蚊属（Stictochironomus Kieffer,1919）斯蒂齿斑摇蚊[S.sticticus（Fabricius,1781]进行了再描述,并纠正Wang（2000）中所记录的辽宁省分布的S.sticicus（Fabricius）为S.akizukii（Tokunaga）。     

Immunologic mechanism of CXCL10 and its receptor involved in endometriosis

AIM: To investigate the immunologic mechanism of CXC chemokine ligand 10(CXCL10) and its receptor CXC chemokine receptor 3(CXCR3) involved in the process of endometriosis (EM). METHODS: Serum samples were collected from 3 groups: EM patients without operation (n=76), EM patients with operation (n=10) and the normal control persons (n=76). CXCL10 and CA125 concentrations were detected by means of ELISA and chemiluminometry. Cell surface antigens on the activated PBMC-CD3 and CXCR3, as well as CXCR3 subgene-CXCR3A and CXCR3B were tested by flow cytometry (FC) and RT-PCR when PBMC was separated from women with EM (n=10) and without EM (n=10), and then activated. RESULTS: Serum CXCL10 concentrations between three groups were significanly different (P<0.05). Compared to normal control group, although the supernatant CXCL10 concentration and CD3+/CXCR3+PBMC number in EM group has no significant difference (P>0.05), highly expressed CXCR3B in EM group rather than CXCR3A was observed. CONCLUSION: CXCL10 in women with EM is low, indicating that it plays a vital role in the process of EM and immune system of the women with EM is defected and impaired. The immunoreactivity of PBMC from both EM patients and normal person is same to activated signal, but the productions are different: PBMC in EM group mainly express CXCR3B but PBMC in normal person mainly express CXCR3A after activation, which may be one of the immune mechanisms that EM escapes from immunological lethal effect of the infected host.     

Soluble expression of a CXCL10-loop3-EGF fusion protein and its anti-tumor activity

AIM: To evaluate the implication of CXCL10-loop3-EGF fusion protein for the activities of targeting tumor and anti-angiopoiesis. METHODS: RT-PCR was preformed to amplify CXCL10 coding sequence from PBMC activated by IFN-γ. CXCL10-loop3-EGF fusion gene, which was conducted by Over-Lap Extention PCR, was hinged up with plasmid pTG19-T, transfected to E. coli DH5α and processed positive colony selection. After ligated with plasmid pET32a(+), recombinant CXCL10-loop3-EGF fusion gene was then transfected to E. coli Origami B (DE3) and induced to express its coding fusion protein his-CXCL10-loop3-EGF. The recombinant fusion protein CXCL10-loop3 -EGF was purified by His-bind affinity chromatograph, enterokinase cleavage, ultrafiltration and dislysis. The transwell chemotatic test and HUVEC angiopoiesis inhibition test were performed to determine the anti-tumor responses and anti-angiopoiesis activity of CXCL10-loop3-EGF fusion protein. RESULTS: CXCL10-loop3-EGF fusion protein was successfully constructed and confirmed by SDS-PAGE analysis and Western blotting. Significant PBMC chematatic activity and HUVEC anti-angiopoiesis activity were observed. CONCLUSION: CXCL10-loop3-EGF fusion protein, which has perfect anti-tumor activity, is successfully constructed.     

4种乡土阔叶树种容器苗生长规律及其培育技术研究

选用苦槠、小叶青冈、枫香与木荷4种乡土优良阔叶树种开展容器苗生长规律及其培育技术研究,结果表明,4个树种的苗高、地径生长规律不尽相同,苗高在7-9月呈直线型生长,9-11月生长趋缓;地径则在6- 11月呈均匀直线型生长。由木荷容器苗在设施大棚内、外培育比较知,优良的设施条件主要影响出苗期与幼苗期苗木的生长量。并提出了容器苗生产培育中控制苗木生长的时期和节约培育成本的技术。     

青钱柳叶片次生代谢产物含量分析

对5个样地青钱柳叶片的鞣质、生物碱、总黄酮、游离蒽醌、绿原酸、皂甙、总酚7种次生代谢产物的含量进行了测定和分析。结果表明:(1)青钱柳叶片中总次生代谢产物的含量在各个样地存在一定的差异,其中天台县华顶山的总次生代谢产物含量最高,其它样地相对较低,其高低顺序为华顶山>钱江源>四明山>庐山>大盘山;(2)相同成分在不同的样地也有差异,青钱柳叶片鞣质含量最高的样地是庐山,生物碱、总黄酮、游离蒽醌、皂甙含量最高的样地是钱江源,绿原酸、总酚以华顶山样地最高;(3)不同样地叶片中各次生代谢产物含量的相关性不高;(4)系统聚类分析显示,5个样地可聚为二类,华顶山、四明山、庐山聚成一类,钱江源、大盘山聚成另一类。     

台州市沿海围涂果园防护林效益调查研究

沿海围涂果园，采用“窄带小网型”防护林营建技术，能降低风速３６．８％－７０．％，减少落果３８．２％－６０．５％，提高空气湿度１％－３％，减少蒸发量１３．７％〉改善果园生态环境，促进果树生长，从而提高产量，该技术宜于南方滨海果园推广。     

水分胁迫对云锦杜鹃光合特性日变化的影响

以盆栽5年生云锦杜鹃苗木为材料,分对照、轻度胁迫、中度胁迫、重度胁迫4组,研究了土壤水分胁迫对云锦杜鹃叶片光合特性日变化的影响。结果表明:随着水分胁迫的加重,云锦杜鹃的光合日变化曲线由典型的双峰型逐渐转变为峰值很小的单峰型,峰值的降低在轻度水分胁迫下由气孔限制引起,而在中度和重度水分胁迫下则由非气孔限制引起。轻度、中度和重度水分胁迫对云锦杜鹃光合特性日变化的影响差异显著。经逐步多元回归得到了净光合速率日变化的回归方程。