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1.
Metal resistance genes are valuable resources for genetic engineering of bioremediation tools. In this study, novel genetic determinants involved in cadmium (Cd) resistance were identified using a small-insert metagenomic DNA library constructed from an arable soil microbiome. A total of 16 recombinant plasmids harboring 49 putative open reading frames (ORFs) were found to be associated with enhanced Cd tolerance. In addition to several ORFs for ion transport/chelation and stress response, most ORFs were assumed to be associated with non-direct metal resistance mechanisms such as energy metabolism, protein/amino acid metabolism, carbohydrate/fatty acid metabolism, and signal transduction. Furthermore, 13 ORFs from five clones selected at random were cloned and subject to Cd resistance assay. Eight of these ORFs were positive for Cd resistance when expressed in Escherichia coli, among which four ORFs significantly reduced Cd accumulation and one increased Cd enrichment of the host cells. Notably, C1-ORF1, potentially encoding a histidine kinase-like adenosine triphosphatase, was the most effective Cd resistance determinant and reduced host Cd accumulation by 33.9%. These findings highlight the vast capacity of soil microbiome as a source of gene pool for bioengineering. The novel genetic determinants for Cd resistance identified in this study merit further systematic explorations into their molecular mechanisms. 相似文献
2.
利用FNL再分析资料、卫星资料和自动站资料对2019年7月22~23日京津冀一次区域性暴雨过程进行了环流形势、卫星云图、动热力结构的分析。结果表明:此次暴雨发生在500hPa两槽一脊、副热带高压北抬、低空急流的大尺度环流背景下。西南方向的水汽输送和黄渤海的补充水汽为暴雨提供了良好的水汽条件,在河北中部的水汽辐合抬升则为暴雨提供了抬升条件。此次暴雨的产生受中尺度对流云团的连续生成合并影响,降水效率高。在暴雨中心整层表现为低层辐合、高层辐散,上升速度中心明显,使得高层的抽吸作用更强,更有利于上升运动的维持与发展,为降水强度增大提供了有力的动力条件,高空弱冷空气的入侵对能量锋区不稳定能量的释放起到了触发作用。 相似文献
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为增加河北二季作区马铃薯品种的多样性,选取适合河北二季作区种植的早熟、特色马铃薯新品系,以黑金刚为对照,对参试马铃薯生长期、商品薯性状及产量等进行了综合评价。结果表明:新品系S4-1-6每667 m~2商品薯产量2 132.28 kg,比对照增产12.23%,综合抗病性较强,薯块长椭圆形,紫黑皮紫肉;S6-1-1每667 m~2产量2 105.51 kg,比对照增产10.82%,综合抗病性强,薯形椭圆,红皮粉红肉。2个彩色马铃薯新品系适宜做土豆泥、土豆饼,丰富了百姓的餐桌,为当地马铃薯种植增加了品系选择。 相似文献
5.
TRMM在海河流域南系的降水估算精度评价及其对SWAT模型的适用性 总被引:3,自引:3,他引:0
准确估算区域降水对水文过程评价和水资源管理意义重大。为评估TRMM 3B42V7降水产品在海河流域南系的估算精度及其在土壤和水评估模型(Soil and Water Assessment Tool,SWAT)中的适用性,利用28个气象站降水观测数据(2007-2016年)和101个雨量站观测数据(2010-2016年)开展研究。研究表明:站点尺度上,3B42V7降水产品对月降水估算的均方根误差小于15 mm,平均误差小于8.5 mm;在湿润季节的估算精度更好。流域尺度上,日降水估算精度较差,相关系数小于0.6。分区尺度上,3B42V7能够很好地捕捉到不同等级降水强度,但对微量降雨有所低估;山区和平原的年降水量均出现高估现象,平原区较为突出;此外,3B42V7能够较好地捕捉到研究区内极端降水的时间和空间分布。分2种情景进行水文模拟,利用月平均流量对模型进行校准和验证,在情景Ⅰ中,验证期模拟结果较好,决定系数在0.56~0.96之间,纳什效率系数在-11.09~0.94之间。TRMM 3B42V7可为海河流域及其类似区域的水资源管理提供参考。 相似文献
6.
为了解决石家庄地区春甘薯—秋白菜生产中品种不配套、栽培技术不规范、病虫害较为严重等问题,充分发挥甘薯节水抗旱、营养均衡、祛病保健,白菜营养丰富、品质柔嫩、口味颇佳的独特优势,特编写了《春甘薯—秋白菜复种栽培技术规程》,详细规范了春甘薯—秋白菜复种栽培的基础条件、茬口安排、品种选择以及春甘薯栽培、秋白菜复种的技术要点。确保了每667 m^2春甘薯产量在2000 kg以上,大白菜产量在4000 kg以上,每667 m^2种植效益比传统模式增加1000元。为种植春甘薯、秋白菜的菜农和蔬菜生产企业提供了技术支撑。 相似文献
7.
Shen Congcong Ma Dawei Sun Ruibo Zhang Benyao Li Delin Ge Yuan 《Journal of Soils and Sediments》2019,19(10):3442-3452
Journal of Soils and Sediments - Coal exploitation inevitably brings a chain of ecological problems, e.g., land destruction and biodiversity decrease. Most previous studies have investigated the... 相似文献
8.
The water-saving potential of using micro-sprinkling irrigation for winter wheat production on the North China Plain 下载免费PDF全文
Li-chao ZHAI Li-hua LÜ Zhi-qiang DONG Li-hua ZHANG Jing-ting ZHANG Xiu-ling JIA Zheng-bin ZHANG 《农业科学学报》2021,20(6):1687-1700
The shortage of groundwater resources is a considerable challenge for winter wheat production on the North China Plain. Water-saving technologies and procedures are thus urgently required. To determine the water-saving potential of using micro-sprinkling irrigation(MSI) for winter wheat production, field experiments were conducted from 2012 to 2015. Compared to traditional flooding irrigation(TFI), micro-sprinkling thrice with 90 mm water(MSI1) and micro-sprinkling four times with 120 mm water(MSI2) increased the water use efficiency by 22.5 and 16.2%, respectively, while reducing evapotranspiration by 17.6 and 10.8%. Regardless of the rainfall pattern, MSI(i.e., MSI1 or MSI2) either stabilized or significantly increased the grain yield, while reducing irrigation water volumes by 20–40%, compared to TFI. Applying the same volumes of irrigation water, MSI(i.e., MSI3, micro-sprinkling five times with 150 mm water) increased the grain yield and water use efficiency of winter wheat by 4.6 and 11.7%, respectively, compared to TFI. Because MSI could supply irrigation water more frequently in smaller amounts each time, it reduced soil layer compaction, and may have also resulted in a soil water deficit that promoted the spread of roots into the deep soil layer, which is beneficial to photosynthetic production in the critical period. In conclusion, MSI1 or MSI2 either stabilized or significantly increased grain yield while reducing irrigation water volumes by 20–40% compared to TFI, and should provide water-saving technological support in winter wheat production for smallholders on the North China Plain. 相似文献
9.
简述了伟思富奇恒温恒湿箱以及与之配套使用的神荣露点仪日常维护与保养的注意事项,指出以预防故障为前提的日常维护工作的必要性.分析了设备可能出现的故障情况,并列举排除故障的方法和维修措施. 相似文献
10.