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Abstract: Raphunus sativus L. commonly known as "radish" is a popular vegetable crop used by people all over the world for its culinary and medicinal properties. Enhancement of in vitro regeneration system for radish variety beeralu is needed to further tissue culture studied. Therefore, the present study was carried out to find out the effects of different organic additives on in vitro shoot regeneration of Radish (Raphanus sativus L.) Var. Beeralu. Hypocotyl explants of aseptic plantlets were cultured on MS basal medium supplemented with 2.5 mg/L BAP (benzyl adenine) and 0.1 mg/L NAA (1-nphthaleneacetic acid) with different natural additives; 20% coconut water, 20% coconut milk, 10% grind spinach leaves, 10% grind potato tubers, 10% grind carrot, 5% rice flour, 10% green gram, 10% grind pumpkin, 10% banana fruit, 10% orange and control (without any additives). Complete randomized design (CRD) with five replicates was used. After one month the numbers of regenerated shoots were counted and statistical analysis was carried out using the Student Newman-Kuells Means Separation Test of SAS program (9.1.3). The highest mean number of shoots (12 shoots/explant) from Radish (Raphanus sativus L.) Var. Beeralu observed in MS basal medium with 2.5 mg/L BAP and 0.1 mg/L NAA with 10% orange juice whereas the 2nd highest shoots were obtained with 20% coconut water. The lowest number of shoots (0 shoot/explant) was observed from medium with carrot juice and pumpkin juice, but they induced callus formation. Media with grind spinach leaves, rice flour, green gram, grind potato tubers and banana inhibit the shoot regeneration.  相似文献   
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为了评价松果菊Echinacea purpurea L.根外植体的再生能力,将从松果菊无菌小苗得到的根外植体和叶片以及叶柄外植体接种到含有不同种类和浓度的细胞分裂素和生长素的培养基上,诱导不定芽的再生.结果表明,在多数情况下,根外植体的再生能力显著高于叶片,和叶柄类似.0.3 mg/L的苄基腺嘌呤和0.01 mg/L的萘乙酸是诱导根外植体不定芽再生最合适的激素种类和质量浓度组合.根外植体培养的不定芽再生频率为100%,每个根外植体得到再生芽1.75个.当把这些由根再生的不定芽从母体组织切开并转移培养到含有0.01 mg/L萘乙酸的培养基后,很容易生根并成为完整的植株.可见根是组培快繁松果菊理想的外植体材料.  相似文献   
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