青海省祁连圆柏天然林单木生物量模型构建

利用祁连圆柏整株生物量与生长指标数据，为估算祁连圆柏林的生物量估算提供参考。通过野外调查，共获取了63株祁连圆柏天然林样木生物量与生长指标实测数据。用其中50株样木数据进行回归模拟，用其余的13株样木数据对模型可靠性进行检验，构建器官生物量与生长指标间的回归模型。结果表明，祁连圆柏单木水平下，树干生物量模型的R²_adj为0.96，均方根、模型有效性和残差系数分别为0.50、0.85和0.05；枝条生物量模型的R²_adj为0.897，均方根、模型有效性和残差系数分别为0.69、0.80和-0.66；叶生物量模型的R²_adj为0.61，均方根、模型有效性和残差系数分别为0.54、0.86和0.15；根生物量模型的R²_adj为0.93，均方根、模型有效性和残差系数分别为0.12、0.997 和-0.01。在调查数据范围内构建的模型较好地反映了祁连圆柏生物量与生长指标间的关系，形式简单、使用方便；与实测值相比，树干与叶生物量模拟值偏小，枝和根偏大。     

基于视觉里程计的森林样地调查系统研究

以视觉里程计技术恢复连续摄影序列图像位姿,并以恢复位姿的图像为基础构建样地调查系统。该系统通过对图像位姿尺度恢复、定义样地坐标系、标记立木等过程估计样地中立木位置及胸径。用相机对12块半径为7. 5 m的圆形样地进行连续摄影,获取有序图像序列,并使用构建的样地调查系统对图像序列进行处理,以获取样地中立木位置及胸径。实验结果表明,所有样地立木位置估计值x轴与y轴方向的偏差(BIAS)分别为0. 04、-0. 03 m,均方根误差(RMSE)分别为0. 21、0. 17 m;样地中立木胸径估计值的BIAS及RMSE分别为0. 09 cm(0. 51%)和0. 88 cm(5. 03%)。     

疏花对无核荔枝果实和树体营养的影响

为了研究疏花对无核荔枝果实和树体营养的影响,以不疏花为对照,在初花期对其进行疏花处理,统计疏花量、记录果实发育情况及成熟日期,并测定果实成熟时的品质和整个过程中树体叶片营养变化情况。据统计,每株树的平均疏花量达到80%-90%左右,疏掉的小穗数、支穗数和侧穗数分别为89.81%、89.74%、81.82%。试验结果表明,疏花使无核荔枝的果实发育情况及果实成熟时的可食率、单果重、总糖、维生素C、还原糖均高于对照；果实成熟时,平均单株产量提高70.59%；疏花处理的果实成熟期较对照提前7天左右；该过程中树体叶片N、P、K、S、Ca、Mg含量在前期高于而后期稍低于对照。疏花加快了无核荔枝果实生长发育,并提高了果实的产量和品质。     

渭-库绿洲土壤剖面盐分分布特征及驱动因子分析

【目的】监测渭-库绿洲土壤盐渍化的空间分布特征,探究驱动因子作用机理,对当地因地制宜进行土壤盐渍化调控。【方法】采用决策树、克里金插值和灰色关联度分析研究了渭-库绿洲土壤盐渍化的剖面分布特征,着重分析了样本点海拔、植被覆盖度、地下水位、TWI(地形湿度指数)、地下水矿化度5个驱动因子对土壤盐渍化的影响。【结果】①研究区表层土壤(0～10 cm)属于重度盐渍化土壤,10～20、20～40、40～60 cm各深度剖面土壤属于中度盐渍化土壤。土壤EC_(1:5)有强的空间变异性,其分布格局受灌溉等人为驱动因素的影响较大。②绿洲内部(即耕作区)表层土壤属于非盐渍化区域,绿洲东部10～20、20～40、40～60 cm土层有轻、中度的盐渍化现象。绿洲内部表层以下土壤盐分高于表层,绿洲存在潜在的盐渍化风险。耕作区外围绿洲-荒漠交错带区域各剖面层均属于盐渍化区域,随着剖面深度的增加,盐渍化程度在不断减弱。③样本点海拔、植被覆盖度、地下水位、TWI、地下水矿化度与土壤EC1:5的灰色关联度大小次序为:0～10 cm土层:地下水矿化度>TWI>样本点的海拔>植被覆盖度>地下水位;10～20、20～40 cm土层:地下水矿化度>样本点的海拔>TWI>植被覆盖度>地下水位。【结论】渭-库绿洲土壤盐渍化主要分布在绿洲-荒漠交错带区域,土壤盐分表聚强烈,地下水矿化度是造成该研究区土壤盐渍化问题的首要原因。     

Direct quantitative real‐time PCR assay for detection of the emerging pathogen Neonectria neomacrospora

The epidemic outbreak in northern Europe of Neonectria neomacrospora, the causal agent of dieback in Abies spp., led the European and Mediterranean Plant Protection Organization (EPPO) to include the pathogen on its alert list in 2017. Effective monitoring of this pathogen calls for a rapid and sensitive method of identification and quantification. A probe‐based real‐time PCR (qPCR) assay based on the β‐tubulin gene was developed for the detection and quantification of N. neomacrospora in infected wood samples, and directly for ascospores. This study presents the first published species–specific molecular detection assay for N. neomacrospora. The analytical specificity was validated on taxonomically closely related fungal species as well as on 18 fungal species associated with the host (Abies sp.). The analytical sensitivity was tested on naturally infected wood, on purified pathogen DNA in a matrix of host DNA and on N. neomacrospora ascospores for detection of airborne inoculum. The latter was tested both with a DNA extraction step prior to qPCR and without DNA extraction by direct qPCR on collected ascospores. The assay was specific to N. neomacrospora, with a sensitivity of 130 fg purified DNA, or 10 ascospores by direct qPCR. Omitting DNA extraction and amplifying directly on unpurified ascospores improved assay sensitivity significantly.     

乡土阔叶树种在碳汇造林中的应用及优势分析

随着我国社会经济的发展,逐步暴露出了许多的生态环境问题。而为了维持生态的平衡,就要提高植树造林的力度,这其中碳汇造林就是植树造林工作的延伸。在碳汇造林中乡土阔叶树种发挥着重要作用,针对乡土阔叶树种在碳汇造林中的应用进行分析,对项目中出现的问题进行优化。     

南方地区甜樱桃不同品种及树形组合筛选

以甜樱桃主栽品种“布鲁克斯”、“红蜜”、“拉宾斯”、“萨米脱”、“黑珍珠”、“秦林”、“06-7”为试材,研究在南方山地条件下不同栽培模式对生长量、果实品质及产量的影响。结果表明：在高海拔条件下甜樱桃物候期相应推迟7-14天；不同树形树势存在显著差异,UFO树形树势良好,且对于果实品质提高有一定作用；UFO树形可显著提高“布鲁克斯”、“06-7”、“萨米脱”等品种产量。该研究为甜樱桃在浙江等南方地区推广合理树形提供参考依据     

茶树植保机械及减量施药技术研究进展

农药防治是茶树病虫害综合防治的重要组成部分,其在病虫害突发或爆发时具有快速高效的防治优势。茶树叶片表面具有亲水性,常量施药会造成茶叶农残超标、生态环境破坏等问题,实现茶树减量施药是减少茶叶农残的有效手段。系统综述了茶树生物特性、茶树病虫害预测诊断及防治方法、茶树植保机械及施药技术,强调提高茶树低容量喷雾的农药有效利用率是实现茶树减量施药的关键。针对目前茶园地面工况复杂及农药利用率低的问题,本文从低容量仿形喷雾机、茶树病虫害喷雾决策及智能终端等六个方面提出茶树病虫害施药技术及装备的研究建议,指出低量化、精准化及智能化是未来茶树植保喷雾机械及施药技术的发展方向。     

药材植物淋出物对油松枯落物分解和土壤酶活性的影响

【目的】研究油松(Pinus tabuliformis)林下9种药材植物淋出物对油松枯落物分解和土壤酶活性的影响,为选择适宜油松林下种植的药材植物及适宜的林药复合模式提供依据。【方法】以秦岭山区广泛分布的典型油松林及林下9种常见药材植物蒲公英(Taraxacum mongolicum)、地丁(Corydalis bungeana)、薄荷(Mentha haplocalyx)、鱼腥草(Houttuynia cordata)、细辛(Asarum sieboldii)、荆芥(Nepeta cataria)、金银花(Lonicera japonica)、绞股蓝(Gynostemma pentaphyllum)和夏枯草(Prunella vulgaris)为研究对象,通过药材植物茎叶淋出物(以水浸提液模拟)喷浇油松枯落物进行分解试验,研究药材植物茎叶淋出物对油松枯落物分解速率、养分(C、N、P、Cu、Zn和Mn)释放和土壤酶活性的影响。【结果】蒲公英、地丁、薄荷、鱼腥草、金银花和荆芥的茎叶淋出物均显著抑制了油松枯落物的分解及总体养分释放(除金银花茎叶淋出物对N释放、荆芥茎叶淋出物对N和P的释放影响不显著外)。蒲公英、地丁、薄荷、鱼腥草和金银花茎叶淋出物均显著降低了土壤多酚氧化酶的活性,金银花茎叶淋出物降低了土壤中羧甲基纤维素酶活性,荆芥茎叶淋出物均显著降低了土壤中羧甲基纤维素酶和β-葡萄糖苷酶的活性。【结论】细辛、绞股蓝和夏枯草可与油松进行林药复合种植,其余6种药材植物应避免在油松林下种植或降低种植密度。     

Intestinal Microbiota in Large Yellow Croaker,Larimichthys crocea,at Different Ages

The polymerase chain reaction–denaturing gradient gel electrophoresis (PCR‐DGGE) of 16S ribosomal RNA gene was used to investigate bacterial communities in the intestines of large yellow croaker at six different ages (12 d, 18 d, 26 d, 40 d, 3 mo, and 1 yr old) as well as within the corresponding feed and culture water. In addition, Illumina Miseq sequencing was utilized to compare intestinal microbiota between 12‐d‐old and 1‐yr‐old individuals. PCR‐DGGE results revealed that the culture water had the highest bacterial diversity, followed by the feed, while the intestines had the lowest diversity. The intestinal microbiota at six ages changed severely; however, the change did not follow any trend. The large yellow croaker intestines harbored specific bacterial communities that differed from those in both feed and water. Illumina Miseq sequencing results revealed that the diversity of intestinal bacteria in 12‐d‐old fish was higher than that in 1‐yr‐old fish, and the bacterial composition differed significantly between them. γ‐Proteobacteria and Pseudoalteromonas supplied the most abundant phylum and genus in the 12‐d‐old fish intestine. However, in the 1‐yr‐old fish intestine, Firmicutes and Clostridium were the most dominant, respectively. The study may contribute to a better understanding of gut microbiota and dynamics of the large yellow croaker and the relationship with their surrounding environment.