Practical use of response surface methodology for optimization of veterinary antibiotic removal using UV/H2O2 process

Three of the most commonly used veterinary antibiotics—enrofloxacin (ENR), sulfamethoxazole (SMX), and oxytetracycline (OTC)—were chosen as representative antibiotics for UV/H₂O₂ treatments. The objective was to determine the optimization of UV/H₂O₂ to remove antibiotics from aquaculture discharge water using response surface methodology. The degradation of the antibiotics was investigated under varying UV/H₂O₂ conditions in environments with different levels of pH, water matrices, humic acid, and constituent ions. The degradation results demonstrated that increasing the H₂O₂ dosage facilitated ENR degradation at a neutral pH while facilitating degradation of SMX and OTC at a slightly acidic pH. The optimum removal conditions for ENR, which was used in all influential effect experiments and the contact tank experiments, was obtained at 10 mM H₂O₂, a pretreated COD of 87.51 mg L⁻¹, and an initial pH of 6.15. Among the tested anions, only the presence of Cl^- showed slight positive effects on ENR degradation, due to the generation of secondary active radicals. During the reaction, the hydroxyl radical (OH) was present at a higher pH while singlet oxygen (¹O₂) was slightly present at a lower pH. The experimental results from H₂O₂ sequential addition indicated that freshly added H₂O₂ could quench the recently generated OH and therefore a high H₂O₂ concentration with frequent adding was not necessary. Our contact system reduced the ENR concentration in both the effluent reservoir and in the UV irradiation zone. The overall results supported the use of the UV/H₂O₂ system to treat remnant antibiotics in the discharge water.     

马尾松叶表微生物多样性

为发掘对林木生长发育有利的优良微生物资源,并筛选适合叶表微生物的收集方法,以马尾松针叶为试验材料,分别用悬摇法和超声波法收集马尾松叶表微生物,用扩增子高通量测序技术、MUSCLE和Qiime软件研究马尾松叶表微生物的多样性。结果表明:扫描电镜观测结果显示,马尾松针叶表面定殖有大量微生物,包括真菌(菌丝及孢子)和细菌。扩增子高通量测序结果表明,马尾松叶表微生物物种丰富,包含细菌运算分类单位(OTUs)490个,真菌OTUs 1273个。马尾松叶表细菌以未分类的蓝细菌属(unidentified_Cyanobacteria)(36.53%)、未分类的拜叶林克氏菌属(unidentified_Beijerinckia)(28.60%)、鞘氨醇单胞菌属(Sphingomonas)(2.35%)为优势属;叶表真菌以枝孢属(Cladosporium)(2.45%)、拟盘多毛孢属(Pestalotiopsis)(0.92%)、无头孢菌属(Capnobotryella)(0.91%)为优势属。针对叶表细菌多样性的研究表明,悬摇法和超声波法均有较高的物种检出度;在叶表真菌多样性的研究中超声波法优于悬摇法,但超声波法样品间数据变异性较大,测定结果不稳定。     

利用响应面法优化椰子风味发酵乳加工工艺

本文以脱脂乳和椰浆为原料进行风味发酵乳的制备。以感官评定为指标,在单因素试验的基础上,选取UHT椰浆添加量、发酵温度和发酵时间进行试验,利用响应面法对椰子风味发酵乳的最佳配方进行优化。结果表明,对风味发酵乳的影响程度最大的因子是UHT椰浆添加量,其次为发酵时间。椰浆发酵乳的最佳配方为椰浆添加量16.5%,发酵温度45℃,发酵时间8h。以此配方制成的椰浆发酵乳,感官评分为45.56,产品颜色均匀,口感顺滑细腻,有浓郁的奶香、椰香风味和滋味。     

基于稻田控水减排的氮肥运筹试验研究

为改善江汉平原地区稻田水肥管理措施,采用田间小区试验,研究了常规淹灌(CF)和浅灌深蓄(SIDS)条件下,农民习惯施肥(FFP)、30%尿素+70%控释参混肥(30%N+70%CRF)和优化减氮施肥(OPT-N)对水稻各生育阶段稻田氮磷流失特征、养分吸收和土壤养分积累的影响。结果表明:与CF处理相比,SIDS处理田间灌溉水量、总用水量、径流量和渗漏量分别降低41.7%,18.5%,45.8%和21.9%,降雨利用率增加16.2%,TN和TP径流流失量分别降低32.6%~35.9%和36.4%~53.1%,渗漏流失量分别降低22.8%~32.0%和16.2%~33.3%,水稻返青期—拔节孕穗期分别占稻田氮磷径流和渗漏流失总量的70%以上。30%N+70%CRF处理、OPT-N处理较FFP处理,TN径流流失量分别降低19.7%~29.2%,15.1%~25.2%,渗漏流失量分别降低25.4%~51.7%,20.9%~26.4%,TP渗漏流失量分别降低18.4%~24.5%,20.4%~31.6%,但TP径流流失量差别很小。从水稻养分吸收和土壤养分积累来看,SIDS处理实际产量相对CF处理可增产4.4%,但对0—40cm土层氮磷养分累积影响不大,30%N+70%CRF处理和OPT-N处理相对FFP处理可增产5.6%和0.4%,且0—20cm土层速效态氮磷养分能维持在一个较高且相对稳定的水平。综上,浅灌深蓄结合30%N+70%CRF施用有利于稻田节水、减少氮磷流失、水稻增产以及土壤肥力改善。     

伊犁绢蒿种子黏液提取工艺及对畜禽粪便污水的净化效果

伊犁绢蒿种子遇水能分泌黏液，黏液物质具有良好的保水性、成膜性、增稠性和分散性，可作为新的天然絮凝剂应用于污水处理中。该文就影响黏液提取率的3个因素提取时间、液料比、提取温度进行探讨，同时用所提取的种子黏液对牛、猪粪便污水进行处理。响应面法结果表明，绢蒿种子黏液在该试验条件下的最高提取率为16.76%，提取条件为：提取时间3 h、液料比60∶1、提取温度64 ℃；畜禽粪便废水处理试验表明，牛粪便废水处理在黏液投加量为50 mg/L时，絮凝率为28%和COD去除率为32.7%；pH值等于6时，絮凝率为46%，COD去除率为44.6%。明矾的联合使用对絮凝有显著的效果，絮凝率和COD去除率最大分别为72.3%和61.3%。猪粪便处理过程中，在黏液投加量为20 mg/L时，絮凝率为30.9%，COD去除率为32.7%，在pH值为7时，絮凝率为29.3%，COD去除率为50%，明矾的联合使用下，絮凝率和COD去除率最大分别为62.6%和68.3%，达到了预想的处理效果。研究可为伊犁绢蒿种质资源在畜禽粪便污水处理方面的开发应用提供参考。     

滦河流域气候变化与人类活动对径流的影响

以滦河流域为研究区域,应用敏感性分析和双累积曲线法分析气候变化和人类活动对研究区径流变化的影响。结果表明:研究区流域径流存在明显的下降趋势,与基准期(1956—1979年)相比,变化期(1980—2013年)径流量减少了52.59mm(50.43%);滦河流域径流对降水的敏感性系数Q/P为0.336 1,对潜在蒸散发的敏感性系数Q/E_0为-0.123 5,气候变化影响量为22.23mm;滦河流域径流变化过程中,人类活动影响量为32.07mm,其贡献率为57.26%,远大于气候变化的影响39.69%,人类活动的影响是导致滦河流域径流减少的主要因素。     

Effects of commercial thinning,log position in the stem,and cutting width on the surface quality of cants produced by a chipper-canter

Fifteen stems of jack pine (Pinus banksiana Lamb.) of 3 commercial thinned plots (control, moderate, and intensive thinning) and 15 stems of black spruce (Picea mariana (Mill.) B.S.P), both coming from the Abitibi-Témiscamingue region – Canada, were cross-cut into three 2.4?m length sections: bottom, middle, and top logs. Logs were processed with a chipper-canter at three cutting widths (12.7, 19.1, and 25.4?mm), producing chips and a three-faced cant. The middle section of the cant was used to evaluate surface quality across the grain on each face. Roughness and waviness parameters and depth of torn grain were recorded. Knot characteristics were assessed in the three cant faces. Poorer surface quality was found in the lower part compared to the upper part of the cant for both species. At larger cutting widths, jack pine logs coming from a natural stand showed lower surface quality compared to logs from thinned stands. Black spruce waviness increased with the cutting width and stem height. These results were attributed to the increase of forces and vibration when cutting at larger cutting widths, which was worsened by the presence of bigger, more numerous knots at the control plot and in the top logs. Black spruce had deeper torn grain compared to jack pine. Their differences in knot characteristics resulted in a maximum torn grain depth favored by the presence of more knots rather than by bigger knot size. Other knot characteristics, such as the position of the knots in the cant face, the insertion angle of the branch and the distribution of the knots around the log, should be studied to better understand the relationship between torn grain formation and knottiness.     

下垫面变化对喀斯特坡地地下产流产沙的影响

通过人工模拟降雨试验,探索表土剥离对喀斯特坡耕地侵蚀产沙的影响。采用钢槽装填土石模拟喀斯特坡地的"二元结构",通过正交试验分析了下垫面类型(坡耕地、裸坡地)、雨强(0.5,0.8,1.3mm/min)和地下孔(裂)隙度(1%,2%,3%,4%,5%)对坡地地下产流、产沙的影响。结果表明:表土剥离导致下垫面土壤物理性质改变,是影响坡地产流、产沙特征变化的主要因素。降雨强度主要影响坡地地下产流量和产沙量,地下孔(裂)隙度主要影响径流和泥沙在地表和地下的分配比例。表土剥离后表层土壤的容重和土壤颗粒分形维数呈显著性增大,导致土壤透水性、孔隙大小等发生改变。相比之下,在0.5mm/min雨强下,裸坡地的地下产流量、产流系数、产沙比重均大于坡耕地,雨强增大至0.8,1.3mm/min后,则是坡耕地大于裸坡地。同时,随着降雨强度的增大,坡耕地产流量和产沙量随雨强的变化顺序为0.5mm/min雨强0.8mm/min雨强1.3mm/min雨强。而裸坡地的产流量大小随雨强的变化顺序为0.8mm/min雨强1.3mm/min雨强0.5 mm/min雨强,产沙量大小随雨强的变化顺序为0.5 mm/min雨强0.8mm/min雨强1.3mm/min雨强。增大地下孔裂隙度能显著(p0.05)的增大两种坡地的地下产流、产沙的贡献量。地下孔裂隙度为1%~3%条件下,2种坡地地下侵蚀产沙比重存在极显著差异(p0.01),随着地下孔(裂)隙度增大至4%和5%,两者间的差异缩小。虽然裸坡地的地下侵蚀产沙量小于坡耕地的,但是其地下产沙比重更大。在喀斯特地区进行开发建设时,应注重地下漏失的防治工作。研究成果能够为喀斯特坡地土壤侵蚀研究和水土流失防治提供重要参考。     

割前摘脱收获机立式离心分离复脱清选装置优化

针对水稻割前摘脱脱出物草谷比低和处理量大的特点,为提高谷物脱出物的处理能力,设计了一种可循环喂入的立式离心分离复脱清选装置并对其进行了响应面优化试验。以喂入速度和螺旋输送器转速为影响因素,以分离率、复脱率和破碎率为试验指标,通过二次正交旋转中心组合试验得到了其性能指标和工作参数的响应曲面模型。利用Design-Expert软件分析各参数对性能指标的交互作用可知,输送器转速对分离率、复脱率和破碎率的影响较大,而喂入速度则较小。通过多目标优化,得到最佳工作参数:输送器转速541.4 r/min、喂入量1.226 kg/s;在该参数组合下的期望结果:分离率为90.4%、复脱率为66.1%、破碎率为0.77%;在该参数组合下的验证性试验结果:分离率为89.4%、复脱率为65.2%、破碎率为0.83%。结果表明立式离心分离复脱清选装置处理效率高,谷物损伤小,能够适应对割前摘脱脱出物的处理要求。     

Exogenous application of humic acid and salicylic acid to alleviate seedling drought stress in two corn (Zea mays L.) hybrids

Efficacy of 1?mM humic (HA) and salicylic (SC) acids on SC 260 and SC 705 corn seedlings to alleviate drought stress via polyethylene glycol was studied via hydroponics at Shiraz University, Iran in a factorial, randomized design, with four replicates each. Under stress, SC 260 had higher electrolyte leakage compared to SC 705, and exogenous application of HA combined with SA decreased SC 705 electrolyte leakage. As a general trend, photosynthetic pigment content, relative water content, root and shoot length, mean number and diameter of central and peripheral root metaxylem, and K⁺ accumulation were higher in SC 705 treated with HA and SA compared to SC 260. Application of HA with SA could be an effective and low cost approach to ensure seedling establishment and plant growth in fields affected by soil drought in the early season, especially for the SC 705 corn hybrid in semi-arid regions.