茶树花青素形成分子机理的研究进展

花青素是由类黄酮合成途径产生的次生代谢产物,含量高时会使茶树新梢呈现红色或紫色。同时,花青素相比儿茶素等具有更明显的抗氧化、预防肿瘤等药理保健作用。文章就茶树花青素合成途径、转录及转录后调控等方面进行综述,以期更好地为高花青素茶树的育种研究提供理论依据。     

新型大跨度非对称塑料大棚内冬季温光变化特征研究

【目的】研究大跨度非对称塑料大棚内冬季温光变化特征,为新型棚体的开发与设计提供思路。【方法】根据跨度和覆盖层数不同,选取5座大棚,分别为17m单层(17-1)、17m双层(17-2)、18m单层(18-1)、18m双层(18-2)和20m单层(20-1),并以塑料拱棚和日光温室为对照,对大棚进行连续气温监测及典型晴天、阴天、雪天条件下气温、土壤温度(20cm深)和光照环境的监测与比较。【结果】①2017-12-01至2018-01-31,18-1、18-2、17-1、17-2、20-1温室冬季平均最低气温分别为4.1,4.9,-1.9,0,-1.8℃,平均最高气温分别为31.7,27.8,32.6,31.9,33.9℃。②典型晴天条件下,18-1、18-2、17-1、17-2、20-1温室最低气温分别为4.4,5.0,-3.4,-2.6,-3.1℃,较室外分别高11.6,12.2,3.8,4.6,4.1℃,较塑料拱棚分别高10.5,11.1,2.7,3.5,3.0℃,较日光温室分别低6.3,5.7,14.1,13.3,13.8℃;典型阴天条件下,最低气温分别为6.3,7.5,1.0,1.6,1.7℃,较室外分别高8.4,9.6,3.1,3.7,3.8℃,较塑料拱棚分别高7.7,8.9,2.4,3.0,3.1℃,较日光温室分别低5.4,4.2,10.7,10.1,10.0℃;典型雪天条件下,最低气温分别为4.9,6.0,-2.7,-1.0,-1.4℃,较室外分别高10.7,11.8,3.1,4.8,4.4℃,较塑料拱棚分别高10.0,11.1,2.4,4.1,3.7℃,较日光温室分别低5.0,3.9,12.6,10.9,11.3℃。典型晴天条件下,18-1、18-2、17-1、17-2、20-1温室20cm深处土壤平均温度分别为12.2,12.3,10.0,11.1,9.6℃,较塑料拱棚分别高6.0,6.1,3.8,4.9,3.4℃;典型阴天条件下,土壤平均温度分别为11.9,12.1,9.5,10.6,9.6℃,较塑料拱棚分别高5.8,6.0,3.4,4.5,3.5℃;典型雪天条件下,土壤平均温度分别为11.2,11.4,9.9,10.5,9.5℃,较塑料拱棚分别高5.4,5.6,4.1,4.7,3.7℃。③对大棚内南北方向气温和光照分布进行比较可知,各试验温室内气温以中部最高,南北次之,分布差异大小排列为17-1温室18-1温室20-1温室18-2温室17-2温室;各试验温室南部光照最好,中部略低于南部,北部最差,透光率大小排序为18-1温室18-2温室20-1温室17-1温室17-2温室。【结论】综合考虑温光环境性能可知,18-2温室大棚的保温性能较好,平均最低气温和土壤平均温度高,无极端低温和高温情况,且南北方向气温、光照分布差异小,透光率高,更适合推广应用。     

创意农业的生成机制及模式类型研究

当前，各界对创意农业的关注持续升温，经济发展推动我国各个产业不断向现代化迈进，在农业的产业现代化进程中，产业升级使传统农业产业结构不断调整；产业融合促进传统农业走向全新发展模式；农业产业链延伸有效提升农业生产各个环节的创新发展空间；创意产业为农业增加文化、创意附加值，创意农业应运而生。根据农业生产的不同产业链环节，创意农业划分为乡村及农业景观创意类、农业生产科技创意类、农产品及包装展示创意类、营销与服务创意类、农产品加工创意类、农业文化遗产创意类六大类型。     

基于卷积神经网络的拖拉机工况识别

农机工况识别在细化农机作业状态和帮助掌握区域污染物排放趋势方面有着重要的研究价值。基于拖拉机不同运行状态下的行驶速度、发动机转速以及实时油耗等时间序列,首次提出将图像识别方法引入到拖拉机工况识别中的思路,并分别应用参数优化的支持向量机与卷积神经网络对实际作业拖拉机工况进行研究。结果表明:(1)基于参数优化的支持向量机可以较好地实现样本点的工况识别且识别准确度达到99.851 9%,但无法实现农机工况的连续性识别,同时无法对农机工况转换阶段进行有效识别。(2)以拖拉机运行速度与发动机转速等信息构建样本图像来描述农机工况变化的数据表达,并在此基础上应用卷积神经网络可以有效实现农机工况的连续性识别,且识别准确率可以达到93.3%。本研究在农机工况识别方面具有一定参考价值,并为后续农机不同工况下区域污染物排放研究提供技术支持。     

有机及常规栽培条件对不同优良食味水稻品种的产量和品质影响试验

通过试验比较各水稻品种在有机及常规栽培条件下的产量和品质,筛选适宜靖江市推广栽培的优良食味水稻品种。试验结果表明:相同品种在常规栽培下整精米率较高,加工品质较好;在有机栽培条件下,稻米外观品质较好,垩白粒率较低,垩白度较低。     

抗马铃薯Y病毒病的VIGS生防剂发酵条件及施用方法优化

病毒诱导的基因沉默(virus-induced gene silence,VIGS)是植物病毒病防治方法之一,能够有效防治马铃薯Y病毒病。以VIGS表达载体发酵液对烟草马铃薯Y病毒病的防治效果为检测指标,筛选最佳发酵液发酵所需的培养基、发酵条件和施用方法。结果表明:VIGS表达载体发酵液最优培养基是YEP培养基;最优发酵条件为装瓶量60 m L/200 m L、初始p H 7.0、培养温度30℃、振荡培养24 h;施用方法以"原液稀释100倍"、"剪叶后喷施+移栽时灌根"和"含PVY CP和HC-Pro基因片段的VIGS载体发酵液混合使用"的施用效果最佳。上述结果为抗马铃薯Y病毒病的VIGS生防剂发酵提供了理论依据,也为其田间应用打下基础。     

Effect of Setting Condition on the Gel-Forming Ability of Rohu

To investigate the setting condition of the gel-forming ability of rohu, optimum setting temperature for strong and weak gels of unwashed and washed rohu gel and optimum setting time for maximum proteolytic activity were investigated. Nine setting temperatures were studied for textural properties and trichloroacetic acid (TCA)-soluble peptide contents. Both unwashed gel (UW-gel) and washed gel (W-gel) showed similar optimum setting conditions for producing a strong gel that was set at 40°C for 30 min, followed by heating at 90°C for 20 min. They displayed different optimum setting conditions for weak gel. Weak gel from the degradation of UW-gel and W-gel formed at 65 and 60°C, respectively. The occurrence of protein degradation of W-gels during setting at 60°C suggested that washing did not remove the endogenous protease, and the degradation of unwashed and washed mince was due to water-soluble protease and myofibril-bound protease, respectively. Eight setting times for maximum proteolytic activity were shown by the TCA-soluble peptide contents, accompanying the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) pattern. Both gels had similar results for setting for 120 min.     

马齿苋ISSR-PCR反应体系与反应条件的优化

[目的]优化马齿苋ISSR-PCR反应体系与反应条件。[方法]以马齿苋为试验材料,采用改良的CTAB法提取总DNA,对ISSRPCR引物进行筛选,同时进行ISSR-PCR反应体系和反应条件的优化,电泳检测ISSR-PCR产物。[结果]8个ISSR引物适合马齿苋的ISSR-PCR分析;最适反应体系为总体系25.0μL,其中2×Taq Platinum PCR Master Mix 12.5μL,10μmol/L引物2.0μL,40 mg/L DNA模板1.0μL,dd H_2O 9.5μL;最适反应条件为94℃预变性360 s;94℃变性60 s,54℃退火60 s,72℃延伸90 s,30次循环;72℃再延伸300 s。[结论]建立了马齿苋ISSR-PCR扩增的最佳反应体系和扩增程序,为进一步研究马齿苋提供基础资料。     

多粘类芽孢杆菌DN-1固态发酵条件优化

[目的]探讨多粘类芽孢杆菌DN-1固体发酵条件。[方法]以活菌数和芽孢率为指标,采用单因素和多因素试验对DN-1菌株的最适培养基成分及发酵条件进行优化。[结果]菌株DN-1最适培养基配比为:15%稻壳,40%玉米粉,20%马铃薯,10%茶渣饼,15%水,0.1%磷酸氢二钾和0.2%硫酸锰。最适发酵条件为:发酵时间72 h,发酵温度37℃,初始pH 6.5~7.0,接种量10%。优化后菌株DN-1活菌数、芽孢率分别达26.3亿/g、92%。[结论]试验结果为DN-1菌株的进一步研究及应用提供了参考。     

Markers for Predicting Overweight or Obesity of Broodmares

Obesity has become of great concern to all equine community from both veterinary and welfare points of view. For estimating obesity markers of brood mares, 17 mares with body conditions were subjected to blood sampling and ultrasound examination to measure rump fat for 6 consecutive weeks. Body length (L), girth (G), and height (H) were measured to estimate body weight (BW), body fat %, body fat mass (BFM) and body mass index (BMI). Mares were classified into three groups according to body condition score (BCS) and rump fat thickness (RF). Overweight mares (O) had BCS >7 and RF >7 mm, moderate (M) had BCS and RF >3 to ≤7, and emaciated (E) had BCS and RF ≤ 3 mm. Glucose, triglycerides, nitric oxide (NO), insulin, insulin-like growth factor-I (IGF-1), leptin, ovarian hormones, and thyroid hormones were measured. Results revealed that BCS, G, L, L × G × H, BW, RF, fat %, and BFM correlated significantly (P < .0001) with body condition. Tetraiodothyronine concentrations of E mares were significantly high (P = .04), but triiodothyronine concentrations tended (P = .07) to be low. Insulin (P = .06) and IGF-1 (P = .07) concentrations tended to be high in O mares. Moderate mares had the highest leptin concentrations (P = .007), but E mares had the lowest P4 concentrations (P = .01). Overweight mares had nonsignificantly high glucose, NO, and triglycerides. In conclusion, back fat and morphometric measurements are the easiest and simple assessment of overweight and obesity. Obese and overweight mares showed slight hyperinsulinemia, hypertriglyceridemia, and hyperglycemia. Hyperleptinemia alone is not indicative of obesity.