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1.
The present study investigated the effects of simulated transport stress on morphology and gene expression in the small intestine of laboratory rats. Sprague Dawley rats were subjected to 35 °C and 0.1×g on a constant temperature shaker for physiological, biochemical, morphological and microarray analysis before and after treatment. The treatment induced obvious stress responses with significant decreases in body weight (< 0.01), increases in rectal temperature, serum corticosterone (CORT), serum glucose (GLU), creatine kinase (CK) and lactate dehydrogenase (LDH) levels (< 0.01), as well as expression of Hsp27/70/90 mRNA (< 0.05; < 0.01). The rat jejunum was severely damaged and apoptotic after mimicking transport stress, which may mainly be related to cell death, oxidation reduction and hormone imbalance determined by microarray analysis. The bioinformatics analysis from the present study would provide insight into the potential mechanisms underlying transport stress-induced injury in the rat small intestine.  相似文献   
2.
The experiment was performed to evaluate the ameliorative effect of ascorbic acid (AA) on some hematological parameters and erythrocyte osmotic fragility (EOF) in horses transported by road. A total of 14 horses, consisting of seven experimental and seven control horses, were used for the experiment. Before the transportation, blood samples were obtained by jugular venipuncture from all the horses. Experimental horses were administered with AA (200 mg/kg dissolved in 20 mL of distilled water per os), whereas the control horses were given 20 mL of distilled water per os. Thereafter, the animals were transported for 6 hours and blood samples collected after transportation. Packed cell volume and hemoglobin concentration were higher (P < .05) in experimental than the control group, whereas total leukocytes reduced significantly (P < .05) in experimental in comparison with the control horses. Lymphocyte, neutrophil counts, neutrophil/lymphocyte ratio, and total protein decreased in experimental horses in comparison with control, but they were not significant (P > .05). Erythrocyte osmotic fragility was lower in experimental than the control at 0.3% NaCl concentration (P < .05). The result of the present study revealed that AA ameliorated changes in hematological parameters and EOF induced by road transport stress, partly because of its antioxidant properties.  相似文献   
3.
牛呼吸疾病综合征及其防治   总被引:2,自引:0,他引:2  
牛呼吸疾病综合征是严重危害国内外肉牛养殖业的一种重要传染病,该病的致病因素复杂,包括支原体、病毒和细菌等特异性病原以及运输应激等多种因素。美国等发达国家对该病的攻关研究开始于上世纪80年代初,其研究和临床防控均已达到较高水平,但仍认为该病将是未来10~20年养牛业所面临的主要疾病。我国对该病的认识和研究尚处于起步阶段,借鉴世界上发达国家的先进经验将有助于提升我国对该病的防控水平。  相似文献   
4.
国内外磷指数评价指标体系研究进展   总被引:1,自引:0,他引:1  
磷是水体富营养化的关键影响因子之一,且农业面源流失的磷是水体中磷的主要来源之一。通过对面源磷的流失风险进行评价,识别其关键区,进而进行针对性管理,是控制面源磷流失的有效手段之一。作为一种评价面源磷流失风险的简单方法,自20世纪90年代以来,磷指数已经在美国、中国及欧洲一些国家得到了广泛的应用。通过采用磷指数法,在评价其流失风险空间分布特征的基础上,可以方便快速地识别出田块或流域内磷流失的关键区。各个国家或地区根据本研究区的情况建立了不同的指标体系,但缺乏统一的磷指数建立标准。从源因子、迁移因子等组成因子及因子等级划分与磷指数计算等几个方面,对国内外的磷指数评价指标体系进行了分析评述,并对其发展进行了展望,旨在为目标研究区磷指数的建立和应用提供借鉴。  相似文献   
5.
Cortisol is involved in the initiation of parturition and we hypothesized that increased maternal cortisol release advances the onset of foaling. Transport is a stressor for horses and induces an increase in cortisol release. To determine stress effects on the time of foaling, late-pregnant mares were transported by road for 3 hours (n = 12) or remained in their stable as controls (n = 4). Starting on day 325 of gestation, saliva and blood samples were taken for cortisol and progestin analysis, respectively. Fetomaternal electrocardiograms were recorded repeatedly. Mares were checked for impending parturition and changes in precolostrum pH. When pH decreased to 6.5, mares were either transported or left untreated. After birth, saliva was collected repeatedly from mares and their foals and heart rate (HR) was recorded. Foals were checked for maturity and health. Gestation length was 337 ± 2 days in stressed and 336 ± 2 days in control mares. Cortisol concentration increased from 3.3 ± 0.9 to 8.4 ± 0.8 ng/mL in transported mares (P < .001) and remained constant in controls. Maternal HR and heart rate variability (HRV) did not differ between groups and neither fetal HR nor HRV changed in response to transport. In transported mares, time from precolostrum decrease to parturition was shorter (40 ± 10 hours) than the respective time in controls (134 ± 49 hours, P < .01). Neither duration of foaling nor times to first standing and suckling of foals or the postnatum increase in HR and decrease in HRV differed between groups. In conclusion, transport-induced maternal cortisol release may have advanced the onset of foaling.  相似文献   
6.
Recent investigations by the Australian Department of Agriculture, Fisheries and Forestry into high mortalities on live export voyages from Australia to the Middle East during the Northern hemisphere summer suggest that animal welfare may be compromised by heat stress. The live export industry has generated a computer model that aims to assess the risk of heat stress and to contain mortality levels on live export ships below certain arbitrary limits. Although the model must be complied with under Australian law, it is not currently available for independent scientific scrutiny, and there is concern that model and the mandated space allowances are inadequate. This review appraises the relevant literature on heat stress in sheep and cattle, including laboratory studies aimed at mimicking the ambient temperatures and humidity levels likely to be encountered on live export voyages. Animal welfare is likely to be very poor as a result of heat stress in some shipments.  相似文献   
7.
蒸腾能使木质部产生不超过-0.1MPa的有限负压,这个负压在木质部内自上而下依次向下传递。水分在导管或管胞内所受的吸附力、毛管力、真空力等综合作用力要大于或等于所受的重力,这个综合作用力可以将水分托住而不受重力作用而下移,使水分在负压作用下以分段移动的方式从根部上升到植物顶端。空穴和栓塞是两个不同的概念,其形成过程、恢复机制都有其本质的不同。空穴的发生与负压和水分的分段移动有密切的关系。  相似文献   
8.
卢军党  杨福增  许兵宗 《安徽农业科学》2010,38(22):12160-12161,12217
通过对农药装盒机说明书输送机构组成零件的分析,运用Pro/ENGINEER Wildfire3.0系统进行三维建模、虚拟装配,在装配过程中对之前所设计零件出现不合理的地方进行修改,使其满足所要设计的功能要求。最后生成爆炸图,使其能清楚地看到该装置的内部结构,为实际装配操作做好准备,大大地提高了农药装盒机设计和制造的效率。  相似文献   
9.
10.
樱桃番茄运输包装件振动冲击性能试验   总被引:1,自引:0,他引:1  
针对目前樱桃番茄储运过程中主要包装形式以及造成破损的情况,选择典型运输包装件,进行了正弦扫频、堆码强度、堆码扫频振动传递性能、垂直冲击和跌落试验。通过对单件包装件的扫频振动试验,测出单件的固有频率为26.61Hz;通过堆码强度试验,测出了包装件的最大堆码层数为9层。通过9层堆码扫频振动传递性能试验确定了底层、中间层和顶层包装件的固有频率分别为36.61、10.76和6.44Hz,得到了各层包装件振动加速度、振动传递率随时间的变化曲线,并探讨了固有频率和振动传递率峰值与堆码高度之间的关系。在此基础上,通过垂直冲击试验,测出了产品的脆值为89.58;通过跌落试验,测出了包装件最大允许跌落高度为580mm。  相似文献   
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