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AIM: In this study, CD147 antibody was used to carry out targeted modification of nanoparticles for protein kinase Cε (PKCε)-siRNA gene therapy to target lung cancer cells. The inhibitory effects of the nanoparticles on the proliferation and invasion of the lung cancer cells were observed. METHODS: The magnetic nanoparticles targeting CD147 protein were assembled as gene vector. The expression of CD147 in the lung cancer cells was observed under laser scanning confocal microscope. The cells were divided into CP group, CN group and LP group as the experimental groups. Targeted nanoparticles were used as CA group. Non-transfected cells were used as control group. The cell transfection was carried out with 250 ng plasmids/well in 6-well plate. The effect of nanocontrast agent on the cell endocytosis was observed under laser scanning confocal microscope. The mRNA expression of PKCε was detected by RT-qPCR. The protein expression of Ki67, MMP3, PKCε, Wnt1 and GAPDH was determined by Western blot. The cell proliferation ability was detected with colony formation assay. The cell invasion ability was detected by Transwell method. RESULTS: The expression of CD147 protein in the human lung cancer A549 cells was confirmed by immunofluorescence staining. The endocytosis of siRNA into the A549 cells in CP group was observed with the highest efficiency as compared with CN group and LP group. The relative mRNA expression of PKCε in the A549 cells of CP group, CN group, LP group and CA group were (9.76±0.18)%, (98.51±0.32)%, (99.17±0.16)% and (99.68±0.11)%, respectively. The difference between CP group and control group was statistically significant (P<0.05). No significant difference among CN group, LP group and control group was observed. The protein expression of PKCε, Ki-67, MMP3 and Wnt1 in CP group was significantly reduced, and the protein expression levels among CN group, LP group and control group had no significant difference. The colony number in CP group was significantly smaller than that in control group (P<0.05). The effective colony numbers in CN group, LP group and CA group had no significant difference as compared with control group. The number of the invading cells in CP group was significantly less than that in control group (P<0.05). The numbers of the invading cells in CN group, LP group and CA group had no significant difference as compared with control group. CONCLUSION: Nanogene vector targeting CD147 can carry PKCε-siRNA to conduct gene therapy efficiently on the lung cancer cells to achieve effective inhibitory effects on the proliferation and invasion of the lung cancer cells. 相似文献
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CD147基因在家兔肠道的克隆与结构预测 总被引:2,自引:1,他引:1
基于电子延伸序列,克隆并分析兔CD147基因.采用兔十二指肠黏膜组织提取总RNA,进行RT-PCR反应,测序并进行结构预测.克隆的兔CD147基因包含一个完整的开放阅读框架,全长为810 bp,编码由270个氨基酸残基组成的CD147前体蛋白,推导的氨基酸序列信号肽为1~16个氨基酸.氨基酸序列与牛、人、褐鼠、野猪的同源性分别为62.5%,65.9%,59.5%和66.3%,三级结构预测表明CD147具有2个免疫球蛋白类似区.试验成功克隆了兔CD147基因,注册到GenBank(Accession.EU650668),并预测其三级结构,为进一步研究其生物学功能奠定基础. 相似文献
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稀土元素钷(147Pm)在食草动物中的富集性 总被引:4,自引:0,他引:4
以1 4 7 Pm ( N O3 )2 溶液 (质量浓度32×10- 3 μg/m L, 放射性比活度为962 k Bq/m L) 喷施牧草, 并用其饲喂食草动物 (豚鼠), 应用核素示踪技术研究豚鼠对1 47 Pm 的富集性。结果表明, (1) 喷施于牧草的14 7 Pm 被动物摄入后, 大部分通过粪便较快排出, 连续饲喂25 d 后, 其排出率为6105% 和7554% ; 连续饲喂13 d 后以普通饲料继续喂养31 d,1 4 7 Pm 排出率为6892% 和7582% 。(2) 滞留于鼠体的1 4 7 Pm 在脏器组织间呈不均匀分布, 其中, 肌肉内含量较低, 骨髓、骨骼、心脏和脂肪中较高, 具有明显的选择性蓄积; 睾丸和肾脏滞留量亦高, 但是终止摄入后排出较快。 相似文献
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基于MSP430F147低功耗单片机节能灌溉系统研究 总被引:1,自引:0,他引:1
【目的】农业用水浪费较严重,灌溉水利用系数较低,很多地区存在水资源匮乏的问题,所以,走节水节能型农业发展之路是必然趋势。【方法】建立以MSP430F147为核心的单片机节能灌溉系统的控制模型,该系统平时处于休眠状态,固定周期进入活动模式,耗能低,从而实现系统的低功耗。【结果】该系统通过测量土壤介电常数确定土壤湿度,然后根据土壤湿度值来确定作物的灌溉用水量,单片机定时控制灌水量,从而达到节水节能的目的。【结论】该系统节水节能,同时通信方式采用无线通信方式,传输距离远,安装方便并减少了通信线路安装的成本,符合未来通信的发展方向。 相似文献
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目的探讨鼻咽癌细胞中CD147、E-cadherin的表达及其意义。方法用免疫组化SP法检测55例鼻咽癌细胞和37例鼻咽黏膜柱状上皮细胞中CD147、E-cadherin的表达。结果鼻咽癌细胞和鼻咽黏膜柱状上皮细胞中,CD147阳性表达率分别为74.55%和43.24%,组间差异有统计学意义(P〈0.01);E-cadherin阳性表达率分别为41.82%和64.86%,组间差异有统计学意义(P〈0.05)。55例鼻咽癌细胞中CD147与E-Cadherin蛋白表达呈负相关(r=-0.269,P〈0.05)。结论鼻咽癌细胞中CD147异常高表达,而E-cadherin表达下降,二者的联合检测对评估鼻咽癌上皮间质转化及预后有重要意义。 相似文献
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We compare the expression levels of the lactate transporter complex consisting of the lactate transporter, monocarboxylate transporter 1 (MCT1), and its ancillary protein, cluster of differentiation 147 (CD147), in the membranes of red blood cells (RBCs) from two breeds of jumping horses and associate the expression levels of these proteins with their jumping ability. The expression levels of MCT1 and CD147 proteins on the membranes of RBCs collected from 30 show jumping horses of two different breeds were quantified: the Brazilian Sport Horses (n = 17) and the European Warmbloods (n = 13). The levels of MCT1 and CD147 in the RBC membranes were measured by western blot using horse-specific antibodies. Statistical analyses included unpaired Student t test and chi-squared test. According to the expression levels of MCT1 and CD147 proteins, 88% of the Brazilian Sport Horses were categorized as high lactate transporters (HTs) and the remaining 12% as low lactate transporters (LTs). The opposite was found for the European Warmbloods, where most animals (77%) were classified as LTs and the remaining animals (23%) were classified as HTs. Brazilian Sport Horses express statistically significantly higher levels of CD147 and MCT1 than European Warmbloods. The classification of horses considering the expression of proteins involved in the ability to transport lactate through the complex MCT1-CD147 seems to be breed dependent, with horses that are able to jump higher obstacles showing lower expression of the MCT1-CD147 complex in their RBCs. 相似文献
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AIM:To study the autophagy of prostate cancer PC-3 cells induced by CD147 in vitro. ME-THODS:The method of amino acid starvation to induce autophagy was used. The expression of CD147was detected by Western blotting. To study the functional effects of CD147 on autophagy in prostate cancer PC-3 cells, the down-regulation of CD147expression was induced by the technique of RNAi. The conversion of autophagic marker protein LC3-I to LC3-II was determined by Western blotting. The cell death after starvation-induced autophagy was analyzed by trypan blue exclusion assay. RESULTS:The CD147 expression gradually increased in starvation-induced autophagy. The down-regulation of CD147 significantly increased the expression of autophagy-related protein LC3-II compared with control group. Meanwhile, the cell death rates increased from (19.3±3.1)% and (22.3±3.5)% in control groups to (38.4±3.1)% in silencing the expression of CD147in the PC-3 cells (P<0.05). CONCLUSION:CD147 inhibits starvation-induced autophgy and autophagy death in the prostate cancer PC-3 cells. 相似文献