Determination of reference intervals for metabolic profile of Hanwoo cows at early,middle and late gestation periods

Background

Metabolic profile was initially designed as a presymptomatic diagnostic aid based on statistical analyses of blood metabolites to provide an early warning of certain types of metabolic disorder. However, there is little metabolic profile data available about Korean Hanwoo cows. Therefore, this study aimed to determine the reference intervals of metabolic profile for Korean Hanwoo cows.

Methods

Healthy animals (2,205) were selected and divided into early (day 1 to 95), middle (day 96 to 190) and late (day 191 to 285) period according to their gestating period. Metabolic profile including total protein (TP), albumin (Alb), urea (UREA), glucose (Glu), total cholesterol (T-Cho), long-chain fatty acid (LCFA), aspartate aminotransferase (AST), gamma-glutamyl transpeptidase (GGT), creatinine (Crea), calcium (Ca), inorganic phosphorous (iP) and magnesium (Mg) were analyzed using a TBA-40FR automatic biochemical analyzer. The data of Korean Hanwoo cows were then compared to those of the Japanese Wagyu cows.

Results

Most of the data of the Korean Hanwoo cows were relatively higher than those of Japanese Wagyu cows, with the exception of Glu and GGT. This may indicate that the nutritional level of feed for the Korean Hanwoo cows was higher than that of the Japanese Wagyu cows because of the different feeding system. In particular, relatively higher levels of UREA and LCFA were observed in the Korean Hanwoo cows, and this may also contribute to the low reproduction efficiency.

Conclusions

These findings may provide some theoretical basis for understanding the reproductive and feeding situation of Korean Hanwoo cows.     

文冠果实时荧光定量PCR内参基因的筛选

为筛选适用于文冠果(Xanthoceras sorbifolium Bunge)性别分化过程研究和不同器官的内参基因,本研究以文冠果性别分化关键期的顶芽和侧芽、根、茎、叶、种仁、雌能花和雄能花为材料,通过实时荧光定量PCR(qRT-PCR)鉴定7个候选内参基因(Actin,UBQ,EF-1α,TUB,eIF-4α,18S rRNA和GAPDH)在各样品中的表达量,并评价7个基因的表达稳定性。结果表明,7个候选内参基因在不同样品中的表达量存在明显差异,各基因在雌能花中的表达均显著高于其他材料,在雄能花中的表达处于较低水平;适用于文冠果性别分化过程的最优内参基因为UBQ和eIF-4α,适用于不同器官的最优内参基因为EF-1α和GAPDH。研究结果将对今后进行文冠果性别分化过程研究和不同器官中的基因表达分析提供参考。     

Assessing the sustainability of cropping systems in single- and multi-site studies. A review of methods

The multiple new challenges facing agriculture require the development of innovative cropping systems with high environmental, economic and social performances. Many research programmes are currently focusing on the design of such cropping systems. Some include the multicriteria assessment of cropping systems by diverse methods and approaches. Some of these research programmes are supported by experimental or farmers’ networks, generating new opportunities for data analysis and raising new research and methodological questions. In this article, we provide an overview based on a review of 56 articles, comparing the various methods for sustainability assessment in single- and multi-site studies. Articles were classified according to three characteristics: (i) their objectives, (ii) the study design (single- vs. multi-site), (iii) the type of system assessed (fictitious vs. real). Our analysis was structured around four items: (i) the variables used to describe cropping systems and production situations and the use of these variables in the assessment process, (ii) the criteria and associated indicators assessed, (iii) the methods used to explore multiple aspects of the performance of cropping systems, (iv) the use of reference values. We identified key points to be taken into account in multi-site studies. The application of the proposed guidelines to experimental networks should facilitate the identification of high-performance cropping systems and the identification of the drivers of cropping system performance.     

加强图书馆联合参考咨询系统的建设,促进文献资源的开发利用

本文简述了文献传递服务发展的基本过程,概括了联合参考咨询网服务模式与内容,对现阶段UCDRS存在的问题进行分析与探讨,提出了解决问题的方法及优化措施,积极整合各类图书馆信息资源和服务力量,促进公益性联合参考咨询系统的发展。     

Developing socioeconomic indicators for an ecosystem-based fisheries management approach: An application to the Korean large purse seine fishery

Developing socioeconomic indicators for ecosystem-based fisheries management is particularly important. This is because socioeconomic factors have direct effects on ecosystems, and ecosystems have direct effects on socioeconomic factors. Therefore, it is imperative that socioeconomic indicators are developed and evaluated in order to predict changes in ecosystems and to provide advice for effective fisheries management. In this study, socioeconomic indicators have been developed to be combined with biological and ecological indicators, in order to conduct the ecosystem-based fisheries assessment. In terms of socioeconomic indicators, five socioeconomic criteria were considered as important attributes of socioeconomic changes. These were economic production, business conditions, levels of income, the state of the market, and levels of employment. In order to establish reference points for the evaluation of indicators, target reference points and limit reference points were set through a comparison with other industries or other fisheries rather than by using the Traffic Light System (TLS) method, which has been used in many previous studies. In addition, on the basis of the application of developed indicators and reference points to the Korean large purse seine fishery, the socioeconomic conditions of the fishery and the usefulness of the indicators were evaluated and management implications were discussed.     

Optimal utilisation of natural resources for agricultural sustainability in rainfed hill plateaus of Orissa

The climatic data for 17 years from 1988 to 2004 of the rainfed hill plateaus of Kandhamal district of Orissa (India) were analyzed to find out the monthly climatic index from the calculated values of effective rainfall and evapotranspiration. The 80% dependable monthly climatic index was correlated with crop coefficient and suitable cropping period and sequences for the study area were suggested based on it. The extent of investment, net return and soil loss from agriculture were estimated as per the present condition and for the suggested cropping patterns. A mathematical model was formulated for optimal allocation of area to different crop sequences with different objectives viz. minimization of soil loss, minimization of investment and maximization of net return from agriculture and was solved using linear goal programming technique. The model suggested to take up food crops in area of 1,30,777 ha and perennial grass cover in 3223 ha with a cropping intensity of 1.61 resulting in a net return of Rs 1064.775 millions sustaining soil loss to a tune of 9489.67 thousand tons per year. The model was found to be favourable in respect of higher net return of Rs 8862.34 and lesser soil loss of 23.41 tons/ha than the corresponding present values. But more investment of Rs 4489.01 per ha was required to fulfill the objectives.     

Impacts of climate variability on reference evapotranspiration over 58 years in the Haihe river basin of north China

Physically, evaporative demand is driven by net radiation (R_n), vapour pressure (e_a), wind speed (u₂), and air temperature (T_a), each of which changes over time. By analyzing temporal variations in reference evapotranspiration (ET₀), improved understanding of the impacts of climate change on hydrological processes can be obtained. In this study, variations in ET₀ over 58 years (1950-2007) at 34 stations in the Haihe river basin of China were analyzed. ET₀ was calculated by the FAO Penman-Monteith formula. Calculation of Kendall rank coefficient was done by analyzing the annual and seasonal trends in ET₀ derived from its dependent climate variables. Inverse distance weighting (IDW) was used to analyze the spatial variation in annual and seasonal ET₀, and in each climate variable. An attribution analysis was performed to quantify the contribution of each input variable to ET₀ variation. The results showed that ET₀ gradually decreased in the whole basin over the 58 years at a rate of −1.0 mm yr⁻², at the same time, R_n, u₂ and precipitation also decreased. Changes in ET₀ were attributed to the variations in net radiation (−0.9 mm yr⁻²), vapour pressure (−0.5 mm yr⁻²), wind speed (−1.3 mm yr⁻²) and air temperature (1.7 mm yr⁻²). Looking at all data on a month by month basis, we found that T_a had a positive effect on dET₀/dt (the derivative of reference evapotranspiration to time) and R_n and u₂ had negative effects on dET₀/dt. While changes in air temperature were found to produce a large increase in dET₀/dt, changes in other key variables each reduced rates, resulting in an overall negative trend in dET₀/dt.     

Measuring versus estimating net radiation and soil heat flux: Impact on Penman–Monteith reference ET estimates in semiarid regions

The standardized ASCE Penman–Monteith and FAO-56 equations were used to estimate reference evapotranspiration (ET₀) using estimated and measured net radiation (R_n) and soil heat flux (G), based on hourly and daily meteorological data. The estimates were evaluated against lysimeter measurements. The results indicate that using measured or estimated values of R_n and G can have significant effect on the accuracy of the ET₀ estimations, especially when calculations were made on an hourly basis. The FAO-56 version performed very well during the irrigation season on a daily basis. The use of measured R_n and G did not improve ET₀ estimation on a daily basis, therefore, the use of estimated R_n and G appears to be dependable when calculations are based on 24-h weather data. When daily ET₀ was calculated from hourly estimations, the results were different depending on the version used. The ASCE version was more accurate, especially when R_n and G were measured. Therefore, measurement of R_n and G may have potential to improve estimation only when daily ET₀ is calculated from hourly estimations. The PM FAO-56 version was always a little less accurate than the ASCE version. For hourly calculations, using a constant surface resistance (as in FAO-56 version), the PM method underpredicted for high evaporative demand and vice versa. The ASCE version performed better than PM FAO-56 version when R_n and G were measured and estimated. Therefore, ASCE version tended to provide quite accurate values of hourly ET₀, even using estimated values of R_n and G. As conclusion, the methods proposed by FAO-56 for estimating R_n and G tended to produce accurate estimates for daily and hourly ET₀ under semiarid conditions and can be used with some degree of confidence for estimating ET₀. In addition, results suggest that the ASCE standardized equation on an hourly basis improved the accuracy of ET₀ estimation with respect to the FAO-56 version.     

春小麦品种农艺性状综合评价的研究

应用灰色关联度分析方法，对育成的6个春小麦品种(系)分别与“参考品种”和对照品种的农艺性状进行综合定量的评价。结果表明，以“参考品种”的综合农艺性状评判出的730-1品系优于以对照品种为参照评判出的730—2品系，该结果与田间表现相一致。