棉花不同时期去叶源对库源关系影响的初步研究

通过对棉花不同时期去主茎叶、叶枝叶、果枝叶和全部叶片的研究,初步探讨了棉花叶源对库的影响.结果表明:无论蕾期还是盛铃期,叶片是棉花光合产物的主要来源,没有叶片棉花就不能正常生长,也很难保证其产量;不同部位的叶片中,主茎叶的作用显著,蕾期去主茎叶后株高日增长量减少0.5 cm以上,干物质积累明显下降,盛铃期则导致棉铃大量脱落,成铃平均减少2个以上;去果枝叶的作用相对较小,去叶枝叶几乎无影响.     

植物在水分胁迫下脯氨酸积累的研究─—Ⅳ关于植物体内脯氨酸积累的直接触发因子

依据干旱胁迫下数种植物幼苗体内游离脯氨酸、叶气孔、叶水量的变化，剖析了植物与环境因子、气孔因子、氧气因子、酶因子及能量因子之间的相互依存关系，提出了ＰＲＯ积累系统（ＰＡＳ）。认为水分胁迫下植物体内ＰＲＯ的大量积累是氧化受抑和合成受激共同调控的结果。氧气最有可能为直接触发因子。     

Analysis of Growth and Productivity of Indian Mustard (Brassica juncea) in Relation to FYM, Nitrogen and Source of Fertilizer

A field experiment was conducted during the winter seasons of 1992–93 and 1993–94 at Anand to study the effect of FYM, nitrogen and source of fertilizer on growth and yield of mustard [ Brassica juncea (L.) Czernj & Cosson]. The results showed significant variation in leaf area index (LAI), crop growth rate (CGR), dry matter production and seed yield. The direct effect of farmyard manure (FYM) was conspicuous in improving the growth of mustard. FYM application at 10 tonnes ha⁻¹ significantly increased the LAI, CGR and dry matter accumulation per plant at almost all the stages during first year study (1992–93) and in pooled analysis. Similarly, nitrogen application registered maximum LAI, CGR at 75 kg level and RGR and NAR at 50 kg level at almost all the during both years. Sulphur carrying source (Ammonium sulphate plus single super phosphate) increased all stages growth characters. Maximum dry matter accumulation per plant and seed yield were recorded with highest levels of FYM (20 tonnes ha⁻¹), N (75 kg ha⁻¹) and source having S. Seed yield was strongly associated with LAI and dry matter accumulation per plant at all the stages.     

陆地棉功能叶不同生育时期酶活性及脂质过氧化作用的研究

对不同熟性棉花品种功能叶几种酶活性及脂质过氧化水平（以丙二醛含量表示）的研究结果表明，超氧物歧化酶（ＳＯＤ）活性在吐絮前呈波动上升，吐絮盛期争剧下降；丙二醛（ＭＤＡ）含量吐絮前缓慢上升，吐絮后明显增加；过氧化氢酶（ＣＡＴ）活性在结铃以前随叶片发育而上升，结铃后急剧下降；过氧化物酶（ＰＯＤ）活性始终呈上升趋势。进一步对ＰＯＤ同工酶进行电泳发现，其酶谱构成随生育进程而变化，早熟品种在开花期酶带条数最多     

The fate of fluroxypyr in the soil in an oil palm agroecosystem

A study on the fate of fluroxypyr in Malaysian agricultural soils was carried out under natural conditions in an oil palm plantation. Three experimental plots in the oil palm estate were selected and three types of treatments were administered, namely fluroxypyr at the recommended dosage, double the recommended rate, and the untreated control. Residues of fluroxypyr in the soil, water, and oil palm leaf were monitored at regular intervals up to 90 days after treatment (DAT). The recovery of fluroxypyr in the water and soil samples ranged from 91–102% when fortified at 1–25 p.p.b. and 91–98% when fortified at 4–50 p.p.b. The recovery from the oil palm leaf ranged from 76–114% when the oil palm leaf sample was fortified at 0.05–1.0 p.p.m. The results showed that fluroxypyr leached down to 50 cm depth at 1 DAT when the plots were treated with either dosage. However, fluroxypyr was not detected in the soil residue at 14 and 21 DAT. Fluroxypyr was not detected in the oil palm leaf samples irrespective of the rates of herbicide application or sampling intervals. The residue of fluroxypyr was detected in water from a nearby stream up to 5 DAT at levels ranging from 0.06–0.21 µg L⁻¹. This result suggests that fluroxypyr persists for a short period in the soil and water ecosystems, but not in the oil palm leaf.     

Movement of Xanthomonas campestris pv. vitians in the stems of lettuce and seed contamination

Xanthomonas campestris pv. vitians , the causal agent of bacterial leaf spot of lettuce (BLS), can be seedborne, but the mechanism by which the bacteria contaminates and/or infects lettuce seed is not known. In this study, the capacity of X. campestris pv. vitians to enter and translocate within the vascular system of lettuce plants was examined. The stems of 8- to 11-week-old lettuce plants were stab-inoculated, and movement of X. campestris pv. vitians was monitored at various intervals. At 4, 8, 12 and 16 h post-inoculation (hpi), X. campestris pv. vitians was recovered from 2 to 10 cm above (depending on stem length) and 2 cm below the inoculation site. Xanthomonas campestris pv. vitians was also recovered from surface-disinfested stem sections of spray-inoculated plants. Together, these results are consistent with X. campestris pv. vitians invading and moving systemically within the vascular system of lettuce plants. To investigate the mechanism of seed contamination, lettuce plants at the vegetative stage of growth were spray-inoculated with X. campestris pv. vitians and allowed to develop BLS. Seed collected from these plants had a 2% incidence of X. campestris pv. vitians external colonization, but no bacteria were recovered from within the seed.     

Genetic variability within Phaeoisariopsis griseola from Central America and its implications for resistance breeding of common bean

The genetic and virulence variability of 112 isolates of Phaeoisariopsis griseola , collected from various locations in Central America, were studied using seven random amplified polymorphic DNA (RAPD) primers and 12 common-bean differential genotypes. Broad molecular diversity ( H  = 0·92) among isolates was found using RAPD markers. Fifty pathotypes were identified on 12 differential bean genotypes, 29 of which were represented by only one isolate. Only 18 pathotypes were found in two or more countries. Pathotype 63-63 was the most virulent and caused leaf spots on all 12 common-bean differential genotypes. Comparison of virulence phenotypes and RAPD profiles to known Andean P. griseola isolates confirmed that all isolates belonged to the Mesoamerican group. Pairwise comparison between individual RAPD loci showed that the majority were in gametic phase linkage disequilibrium, revealing that P. griseola maintains a genetic structure that is consistent with asexual reproduction. The molecular and virulence diversities of P. griseola isolates from Central America imply that using single resistance genes to manage angular leaf spot is inadequate and stacking resistance genes may be necessary to manage the disease effectively.     

Variation of Parthenium hysterophorus in response to soil quality: implications for invasiveness

Summary The performance of Parthenium hysterophorus , native to the tropical Americas and invasive to several countries, was evaluated for response to soil quality. Phenological (six stages) and quantitative growth variables [relative growth rates in height (R_h) and diameter (R_d)] were measured every fortnight. Based on harvest data, the variables, root:shoot (R:S) ratio, specific leaf area (SLA), relative growth rate in biomass (R_w), net assimilation rate (NAR) and dry matter allocation to plant components, were determined. High clay content in soils prolonged the rosette stage, enhanced R_h and R_d and hampered root growth, but promoted biomass allocation to shoots. The extreme degree of plant mortality was observed, with only 33.3% individuals surviving to reproduce in the soils with highest clay contents. This appears to be one of the most important findings that certainly has a major bearing on the range of adaptation of the weed. Seed mass declined whereas seed production increased in relatively coarser soils with the exception of nearly pure sand. In soils rich in clay, plants produced a smaller number of larger seeds. These data suggest that variation in fundamental functional traits would enable P. hysterophorus to adjust to a variety of habitat conditions.     

Leaf, floret and seed infection of wheat by Pyrenophora semeniperda

Infection processes of Pyrenophora semeniperda on seedling and adult wheat leaves and wheat ears were investigated. Almost 100% germination of conidia occurred on seedling leaves, compared with 20–30% on adult leaves. Appressoria formed over the anticlinal epidermal cell walls and haloes always accompanied infection. Sometimes papillae formed within the leaves as a resistance mechanism. Infection hyphae ramified through the intercellular spaces of the mesophyll resulting in cellular disruption. The infection processes on floral tissues were similar to those observed on leaves; however, no infection occurred on anther, stigmatic or stylar tissues. Infection of ovarian tissue occurred both with and without appressoria formation. Hyphae grew mainly in the epidermal layers and appeared unable to breach the integumental layer as no growth was observed in endosperm or embryo tissues. The optimum dew period temperature for conidial germination was 23·6°C, compared with 19·9°C for lesion development, 20·4°C for the production of infection structures on seedling leaves and 23·7°C for floret infection. Leaf disease development occurred in a logistic manner in response to dew period, with maximum infection observed after 21 h compared with > 48 h in seeds. An initial dark phase during the dew period was necessary for infection and temperature after the dew period had an effect, with significantly more numerous and larger lesions being formed at 15°C compared with 30°C. Seedling leaves were found to be more susceptible than older leaves, under both field and controlled environment conditions. Infection of wheat seeds following inoculation of ears, or after harvest burial of inoculated disease-free seeds, was demonstrated. In the latter, 3-week-old seedlings were slightly stunted, whereas older plants were unaffected. The apparent unimportance of this plant pathogen as a cause of leaf disease in relation to its poor adaptation to dew periods and dew period temperature is discussed, along with the importance of its seed borne characteristics.     

引起糖甜菜细菌性叶斑病的萎蔫短小杆菌新致病变种

 1995年在内蒙古临河市新发现了糖甜菜细菌性叶斑病,从病斑所分离的10个细菌菌株经柯赫氏法则验证,均确系该病的病原菌。采用形态观察、表型特征和生理生化特性测定、数值分析、血清学反应、细胞化学成分分析、DNA G+C mol%和DNA-DNA同源性测定进行了鉴定,并与植物病原棒形细菌15个标准菌株进行了比较。该病原菌为革兰氏阳性细菌,不规则短杆状,有一根鞭毛、亚极生或侧生,结合其生理生化特性、细胞化学成分和DNA G+C mol%和DNA-DNA同源性测定结果,认为应属于短小杆菌属(Curtobacterium)的萎蔫短小杆菌(Cur. flaccumfaciens),数值分析也支持这一结论。此外,据血清学反应结果及其对短小杆菌属的其它植物寄主的致病情况,认为该病原菌应是萎蔫短小杆菌种下的一个新的致病变种,定名为Curtobacterium flaccumfaciens pv. beticola pv. nov. Chen et al.,2000(萎蔫短小杆菌糖甜菜致病变种)。