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中链脂肪酸甘油酯已经成为抗生素替代物研究的新热点。文章旨在探究月桂酸、单辛酸甘油酯、单月桂酸甘油酯、辛葵酸甘油酯对大肠埃希菌、金黄色葡萄球菌、肠炎沙门氏菌和鸡白痢沙门氏菌的抑菌效果。结果显示:月桂酸、单月桂酸甘油酯、单辛酸甘油酯对大肠埃希菌有显著(P<0.05)抑制作用;月桂酸、单月桂酸甘油酯、单辛酸甘油酯、辛葵酸甘油酯对金黄色葡萄球菌有显著(P<0.05)抑制作用;月桂酸、单月桂酸甘油酯、单辛酸甘油酯、辛葵酸甘油酯对肠炎沙门氏菌有显著(P<0.05)抑制作用;月桂酸、单月桂酸甘油酯、单辛酸甘油酯对鸡白痢沙门氏菌有显著(P<0.05)抑制作用。其中,月桂酸、单月桂酸甘油酯、单辛酸甘油酯对4种致病菌的作用效果随时间延长而增强。 相似文献
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松鼠葡萄球菌(Staphylococcus sciuri,S.sciuri)是引起奶牛乳房炎(Cow Mastitis)的病原菌之一,其主要致病因子是该菌产生的脱皮毒素C(Exfoliative Toxin C,ExhC)。为了深入了解该基因的分子特征,本试验对从乳房炎患牛牛乳中分离的一株松鼠葡萄球菌ExhC基因(Genbank登录号:MT845354)进行了克隆及序列分析。按GenBank收录的ExhC基因设计并合成引物,利用PCR对ExhC基因进行扩增,并对扩增后的ExhC基因进行测序及分析。结果表明,松鼠葡萄球菌ExhC基因的开放阅读框序列长度为837bp,共编码278个氨基酸。通过Blast模块对ExhC基因进行同源性分析,得到5株相似序列,与ExhC基因序列相比同源性均为99.04%。系统进化树图指示该基因与其余五株相似序列属于不同的分支,遗传距离较远。利用TMHMM对松鼠葡萄球菌ExhC基因837bp序列跨膜区进行预测,结果显示ExhC蛋白的第1~277位氨基酸在细胞膜外,5~25位氨基酸所在位置为跨膜区。通过ExhC氨基酸序列分析和ExhC蛋白二级结构、三级结构预测,推断第97位氨基酸插入Asn以及其他位点氨基酸的突变可能会导致松鼠葡萄球菌功能区变化和毒株毒力的相对变化,这将给松鼠葡萄球菌致病机理的研究奠定基础。 相似文献
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施用有机肥对当归药材性状、产量及抗病性的影响 总被引:1,自引:0,他引:1
为了探寻有机肥对当归成药栽培的影响,采用黑膜覆盖栽培,基施纯有机肥(O,2000 kg·hm-2)、纯化肥(C,磷酸二铵,420 kg·hm-2)、减半量化肥增施有机肥[1/2(O+C)],以不施肥为对照(CK),栽培期测定早期抽薹率、发病率、药材产量和性状指标。结果表明,纯有机肥栽培当归早期抽薹率为8.0%,分别较CK、1/2(O+C)和C降低19.4%、15.2%和7.1%。药材产量则相反,O、C和1/2(O+C)栽培当归药材产量相当,鲜产量分别较CK提高105.9%、84.6%和78.2%。纯有机肥栽培当归根最长,侧根最多,单根最重,药材产量最高。化肥对根的增粗作用最大,但含水量高,根病率高,发病重。不同施肥每hm2鲜药材产量与综合评价指数大小顺序一致,依次为O(9220.6 kg,0.926)>C(9038.5 kg,0.610)>1/2(O+C)(8728.4 kg,0.481)>CK(4897.4 kg,0.190)。每hm2干药材产量排序为O(3149.2 kg)>1/2(O+C)(3098.7 kg)>C(2909.2 kg)>CK(1707.0 kg),与经济收益一致。在岷县道地产区黑膜覆盖栽培,纯施有机肥对当归药材具有显著增效作用,可有效降低早薹率、根病率和发病程度,改善药材性状,提高药材产量和质量,在当归标准化栽培中可推广应用,以改变当归依赖化肥的生产现状,促进绿色有机栽培。 相似文献
5.
82份籼粳稻骨干亲本抗稻瘟病基因的分子检测 总被引:1,自引:0,他引:1
北粳南移在江西省已推行11年,为进一步明确适合江西晚粳常规稻的稻瘟病抗性情况及粳渗籼骨干亲本的选择,本研究利用Pi37/Pi35/Pish、Pi5、Pi2、Pi9、Pia、Pi23、Pigm、Pi33/Pi42、Pi56、Pik/kg(t)/ks/kp/km/kh/43、Pikh共11个抗瘟基因的标记结合井冈山抗性谱的鉴定,对28份骨干粳稻和54份骨干籼稻进行分子标记与抗性分析。结果表明,粳稻中有1份携带其中的7个抗性基因,有5份携带6个抗性基因,有7份携带5个抗性基因,有12份携带4个抗性基因,有3份携带3个抗性基因,抗性频率最高的Pia为0.786,其次为Pikh和Pi9,频率均为0.643,Pigm和Pi37/Pish在这批粳稻材料中的抗性频率为0;籼稻中有8份携带其中的7个抗性基因,有10份携带6个抗性基因,有13份携带5个抗性基因,有17份携带4个抗性基因,有4份携带3个抗性基因,有2份携带2个抗性基因,抗性频率最高的Pi9为0.981,其次Pik/Pikh/Piks频率为0.833,Pigm、Pi37/Pish、Pikh、Pia、Pi5、Pi2、Pi23和Pi33/Pi42的频率分别为0.556、0.537、0.519、0.389、0.352、0.315、0.167和0.111,Pi56在这批籼稻材料中的抗性频率为0。明确这些材料抗瘟基因的分布,将为广谱持久抗性籼粳材料创制骨干亲本的选择与配组指明了方向。 相似文献
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旨在研究果园土壤有机质与土壤氮素养分的相关性与空间分布变化,为果园土壤的可持续利用提供依据。对庆阳苹果主产区30个果园土壤有机质含量和土壤氮素养分进行农化分析。结果表明:0~20 cm果园土壤有机质与全氮含量的相关关系为y=0.055x+0.140,相关系数R2=0.940,土壤有机质与碱解氮含量的相关关系为y=3.061x+26.65,相关系数R2=0.414,土壤全氮与碱解氮含量的相关关系为y=55.28x+18.83,相关系数R2=0.441;20~40 cm果园土壤的有机质与全氮含量的相关关系为y=0.045x+0.250,相关系数R2=0.721,土壤的有机质与土壤碱解氮含量的相关关系为y=2.237x+23.84,相关系数R2=0.158,土壤的全氮与碱解氮含量的相关关系为y=57.47x+5.141,相关系数R2=0.298。 相似文献
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Anna Maria Addamo Karen J. Miller Vreni Häussermann Marco Taviani Annie Machordom 《水产资源保护:海洋与淡水生态系统》2021,31(1):1-14
- 1. When considering widely distributed marine organisms with low dispersal capabilities, there is often an implication that the distribution of cosmopolitan species is an artefact of taxonomy, constrained by the absence of characters for delimiting either sibling or cryptic species. Few studies have assessed the relationship among populations across the global range of the species' distribution, and the presence of oceanographic barriers that might influence gene flow among populations are underestimated.
- 2. In this study, evolutionary and ecological drivers of connectivity patterns have been inferred among populations of the cold-water coral Desmophyllum dianthus, a common and widespread solitary scleractinian species, whose reproduction strategy and larval dispersal are still poorly unknown.
- 3. The genetic structure of D. dianthus was explored using 30 microsatellites in 347 specimens from 13 localities distributed in the Mediterranean Sea and Atlantic and Pacific Oceans.
- 4. Results clearly reveal genetically differentiated populations in the Northern and Southern Hemispheres (FST = 0.16, FSC = 0.01, FCT = 0.15, P-values highly significant), and Chilean and New Zealand populations with independent genetic profiles.
- 5. Marine connectivity patterns at different spatial scales are discussed to characterize larval dispersal and gene flow through the Northern and Southern Hemispheres.
9.
Shutao Dai Jinna Hou Maomao Qin Ziju Dai Xiaojie Jin Shilei Zhao Yanhua Dong Yongxia Wang Zhengqing Wu Zhensheng Lei 《中国油料作物学报(英文)》2021,6(1):28-34
Miniature inverted-repeat transposable elements(MITEs)are a group of DNA transposable element(TE)which preferentially distributed with gene associated regions.Tens of MITEs families have been revealed in Brassica napus genome,they scatter across the genome with tens of thousands copies and produce polymorphisms both intra-and inter-species.Our previous studies revealed a Tourist-like MITE,Monkey King,associated with vernalization requirement of B.napus,however there are still few studies reveal MITE association with agricultural traits in B.napus.In the present study,80 polymorphic markers were developed from 55 MITEs,and used to evaluate genetic diversity in a panel of B.napus accessions consisting of 101 natural and 25 synthetic genotypes.Five agricultural traits,oil content,glucosinolate content,erucic acid content,weight of thousand seeds(WTS)and plant height,were investigated across 3-years field experiments,in addition,two traits,hypocotyl length and root length,were evaluated at the 4-leaf stage in the laboratory.Correlations between the MITE-based markers and seven traits were analyzed,finally,10 polymorphic markers produced by 6 pairs of MITE specific primers were revealed relatively high correlation with 5 traits.Two polymorphic markers were anchored with two candidate genes,BnaA02g13530D and BnaA08g20010D,respectively,which may contribute to glucosinolate content and WTS.This research may contribute to genetic improvement through utilization of MITE-induced polymorphisms in Brassica species. 相似文献
10.
Organic matter fractions within macroaggregates in response to long-term fertilization in calcareous soil after reclamation 下载免费PDF全文
Soil organic carbon(SOC) plays a key role in improving soil quality and optimizing crop yield. Yet little is known about the fate of macroaggregates(0.25 mm) under long-term fertilization and their relative importance in SOC sequestration in reclaimed calcareous soil. Therefore, the effects of mineral fertilizers and organic manure on the mechanisms of organic carbon(OC) stabilization in macroaggregates were investigated in this study. Four treatments were used: unfertilized control(CK), mineral fertilizer(NPK), compost chicken manure alone(M), and mineral fertilizers plus manure(MNPK). Samples from the 0–20 cm layer of soil receiving 11-year-long fertilization were separated into four fractions based on the macroaggregates present(unprotected coarse and fine particulate organic matter, cPOM and fPOM; physically protected intra-microaggregate POM, i POM; and biochemically protected mineral associated OM, MOM) by the physical fractionation method. Compared with the control, the long-term application of NPK had little effect on SOC content, total nitrogen(TN) content, and OC and TN contents of macroaggregate fractions. In contrast, incorporation of organic manure(MNPK) significantly increased SOC(45.7%) and TN(24.3%) contents. Application of MNPK increased OC contents within macroaggregate-extracted fractions of cPOM(292.2%), fPOM(136.0%) and iPOM(124.0%), and TN contents within cPOM(607.1%), fPOM(242.5%) and iPOM(127.6%), but not the mineral associated organic carbon(MOM-C) and nitrogen(MOM-N) contents. Unprotected C fractions were more strongly and positively correlated with SOC increase than protected C fractions, especially for cPOM-C, indicating that SOC sequestration mainly occurred via cPOM-C in the studied calcareous soil. In conclusion, MNPK increased the quantity and stability of SOC by increasing the contents of cPOM-C and cPOM-N, suggesting that this management practice(MNPK) is an effective strategy to develop sustainable agriculture. 相似文献