15N-Urea Efficiency in Maize as Influenced by Humic Substances and Urease Inhibitors Treatments

Urea treated with urease inhibitors (UI) in association with humic substances (HS) is expected to improve urea efficiency by reducing ammonia losses and also provide the benefits of HS such as improve nitrogen (N) recovery by plants. Ammonia volatilization (AV), ¹⁵N recovery efficiency in the soil (NRE) and ¹⁵N use efficiency (NUE) were evaluated in maize (Zea mays L.) in a greenhouse pot trial. Treatments consisted of ¹⁵N-urea treated with UI (0, 0.4% boron (B) +0.15% copper (Cu), 0.64% B, and NBPT (N-(n-butyl) thiophosphoric triamide)) and three levels of HS (0%, 0.6%, and 1.2%). A control treatment (without N) was also included. N treatments were applied at V4 (vegetative leaf stage 4) on soil surface. HS was not efficient on AV reduction, NBPT had the greatest reduction in AV, and B had higher efficiency on AV reduction than Cu. At V8 (vegetative leaf stage 8), NBPT had the greatest NUE followed by B. At VT (tassel fully emerged), NUE from urea treated with NBPT reduced when increased levels of HS, and NBPT had the greatest NRE. UI in association with HS treated-urea does not reduce AV and it does not improve NUE by maize in vegetative stages.     

风化煤改变黄河三角洲盐渍化土壤溶液组分的过程

黄河三角洲盐渍土改良具有重要的经济和社会意义。风化煤(0%、1%、3%、5%)添加到不同含盐量(1.0 mg·g~(-1)、7.5 mg·g~(-1)、35.3 mg·g~(-1))的滨海盐渍化土壤后,可降低土壤浸提液的钠吸附比(Sodium adsorption ratio,SAR)及Cl~-/SO_4~(2-)比,有助于减轻盐渍危害。7.5 mg·g~(-1)含盐量土壤在5%添加下SAR由7.81降为6.61,Cl~-/SO_4~(2-)由10.20降为8.25。风化煤中丰富的羧基等表面官能团及Ca~(2+),可通过三个作用过程改变盐渍化土壤溶液的离子组成,涉及:(1)风化煤固相Ca~(2+)与溶液Na~+离子的交换,从而降低土壤溶液中Na~+的浓度和危害;(2)溶液中Ca~(2+)与土壤交换性Na~+的置换,有助于土壤团聚体的形成和Na~+的淋洗;(3)溶液中的Na~+与风化煤中的溶解性有机质形成络合物,使Na~+的活度和植物有效性降低,间接起到减害作用。此外,钠吸附比和氯硫比可用于在实验室初步判断风化煤等物料用于盐渍土改良的潜力和效果。     

Humic acids stimulate growth and activity of in vitro tested axenic cultures of soil autotrophic nitrifying bacteria

In the present study, the effect of humic acids on activity and growth of Nitrosomonas europaea and Nitrobacter agilis was investigated in vitro under axenic conditions. Humates from compost-stabilized vegetable waste or leonardite were added to the chemolithotrophic culturing medium at concentrations of 0, 5, 50 and 100 mg l^–1. It was found that both types of humic acids increased either NH₄ ⁺ or NO₂ ^– oxidation and cell growth of nitrifying bacteria in a dose-independent manner. By combining these results with data from a comparative growth evaluation of N. agilis based on possible utilization of humates or pyruvate in heterotrophic conditions, evidence was obtained that nitrifiers cannot use humic acids as an alternative carbon and energy source. Thus, the stimulating effect of this fraction of soil organic matter on chemolithotrophic ammonia and nitrite oxidizers might be attributed to an increase in microbial membrane permeability favouring a better utilization of nutrients. Received: 15 April 1996