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1.
《Veterinary parasitology》2015,207(1-2):56-63
Although several techniques exist for the detection of equine tapeworms in serum and feces, the differential diagnosis of tapeworm infection is usually based on postmortem findings and the morphological identification of eggs in feces. In this study, a multiplex polymerase chain reaction (PCR)-based method for the simultaneuos detection of Anoplocephala magna, Anoplocephala perfoliata and Anoplocephaloides mamillana has been developed and validated. The method simultaneously amplifies hypervariable SSUrRNA gene regions in the three tapeworm species in a single reaction using three pairs of primers, which exclusively amplify the internal transcribed spacer 2 (ITS-2) in each target gene. The method was tested on three types of sample: (a) 1/10, 1/100, 1/500, 1/1000, 1/2000 and 1/5000 dilutions of 70 ng of genomic DNA of the three tapeworm species, (b) DNA extracted from negative aliquots of sediments of negative control fecal samples spiked with 500, 200, 100, 50 and 10 eggs (only for A. magna and A. perfoliata; no A. mamillana eggs available) and (c) DNA extracted from 80, 50, 40, 30, 10 and 1 egg per 2 μl of PCR reaction mix (only for A. magna and A. perfoliata; no A. mamillana eggs available). No amplification was observed against the DNA of Gasterophilus intestinalis, Parascaris equorum and Strongylus vulgaris. The multiplex PCR method emerged as specific for the three tapeworms and was able to identify as few as 50 eggs per fecal sample and as little as 0.7 ng of control genomic DNA obtained from the three species. The method proposed is able to differentiate infections caused by the two most frequent species A. magna or A. perfoliata when the eggs are present in feces and is also able to detect mixed infections by the three cestode species. 相似文献
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目前新加坡已建成1个国家图书馆、3个区域图书馆、23个社区图书馆、10个社区儿童图书馆、10个学校和学术馆、14个政府和特别服务馆。这些设施都有有充足的、高素质的人员配备,政府给予的充分的财政支持和一个优秀的领导团队所对新加坡文化、经济起了巨大的推动作用。 相似文献
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Daniel P. Fogarty Benjamin Reinhart Tochko Tzvetkov Ivan Nesch Cooper Williams 《Journal of Equine Veterinary Science》2011,31(7):365-369
We describe and demonstrate the first application of a laboratory-based diffraction-enhanced X-ray imaging instrument for noninvasive equine imaging. A formalin-preserved disarticulated forelimb from a near-term aborted miniature horse fetus was imaged with diffraction-enhanced X-ray imaging. The resultant calculated images—absorption, extinction, refraction, and scattering—are presented, and soft-tissues such as the dorsal digital extensor tendon, articular cartilage, as well as various joint, tendon sheath, and bursa recesses are observed in simultaneous registration with the adjacent dense bone tissue. Radiation dose calculations were performed and a calculated surface dose of 0.6 mGy for the soft muscular tissue was determined for the imaging experiment. 相似文献
8.
Long range (or strategic) planning is an important tool for forest management to deal with the complex and unpredictable future.
However, it is the ability to make meaningful predictions about the rapidly changing future that is questioned. What appears
to be particularly neglected is the question of the length of time horizons and the limits (if any) to these horizons, despite
being considered one of the most critical factors in strategic planning. As the future creation of values lies within individual
responsibility, this research empirically explored the limits (if any) of individual foresters’ time horizons. To draw comparisons
between countries with different traditions in forest management planning, data were collected through telephone surveys of
forest managers in the state/national forest services of the Netherlands and Germany. In order to minimize other cultural
differences, the research in Germany concentrated on the federal state of Nordrhein-Westfalen, which has considerable similarities
with the Netherlands, e.g. in topography, forest types and forest functions. The results show that, in practice, 15 years
appears to be the most distant horizon that foresters can identify with. This is in sharp contrast to the time horizons spanning
decades and even generations that are always said to exist in forestry. The “doctrine of the long run”—the faith in the capacity
of foresters to overcome the barriers of the uncertain future and look ahead and plan for long-range goals—which in many countries
still underlies traditional forest management, can therefore be rejected.
相似文献
Heiner SchanzEmail: |
9.
杂交水稻制种父母本花期相遇诊断技术研究 总被引:2,自引:0,他引:2
以四川全省试验资料为基础,研究了杂交水稻制种父母本生长发育、抽穗开花的规律及影响父母本播差期安排的主要因子,建立了相应的预测模型和四川杂交水稻制种父母本花期相遇微机诊断系统(DSFPMPHRSP),在四川杂交水稻制种生产中推广应用,提高调差精度2~3d,增产杂交稻一代种子150~300kg/hm2。 相似文献
10.
In industrial areas, heavy metals may accumulate in forest soil organic horizons, affecting soil microorganisms and causing
changes in the chemical composition of the accumulated organic matter. The objectives of this study were to test the ability
of near-infrared spectroscopy (NIRS) to detect heavy metal effects on the chemical composition of forest soil O horizons and
to test whether NIRS may be used to quantitatively determine total and exchangeable concentrations of Zn and Pb (Znt, Pbt, Znex, Pbex) and other chemical and microbial properties in forest soil O horizons polluted with heavy metals. The samples of O horizons
(n = 79) were analyzed for organic C (Corg), total N and S (Nt, St), Znt, Pbt, Znex, Pbex, basal respiration (BR), microbial biomass (Cmic) and Cmic-to-Corg ratio. Spectra of the samples were recorded in the Vis-NIR range (400–2,500 nm). To detect heavy-metal-induced changes in
the chemical composition of O horizons principal components (PC1–PC7) based on the spectral data were regressed against Znt + Pbt values. A modified partial least squares method was used to develop calibration models for prediction of various chemical
and microbial properties of the samples from their spectra. Regression analysis revealed a significant relationship between
PC3 and PC5 (r = −0.27 and −0.34, respectively) and Znt + Pbt values, indicating an effect of heavy metal pollution on the spectral properties of the O horizons and thus on their chemical
composition. For quantitative estimations, the best calibration model was obtained for Corg-to-Nt ratio (r = 0.98). The models for Corg, Nt, and microbial properties were satisfactory but less accurate. NIRS failed to accurately predict St, Corg-to-St, Znt, Pbt, Znex, and Pbex. 相似文献