采用多元统计分析方法构建南极磷虾粉品质评价体系

南极磷虾是一种重要的战略性海洋生物资源，营养价值高，资源量大，开发利用前景广阔。为了构建南极磷虾粉品质评价体系，为南极磷虾粉品质的科学评价提供依据。通过检测15项南极磷虾粉品质指标，采用因子分析筛选品质评价指标，应用概率分布分级品质评价指标，通过层次分析确定品质评价指标权重，利用K-均值聚类分析和判别分析建立南极磷虾粉品质判别函数。结果表明：1）南极磷虾粉品质指标之间离散度有差异，变异系数在8.89%～84.46%；2）从15项指标中选择6项作为南极磷虾粉品评价指标（蛋白质、脂质、盐分、赖氨酸、生育酚、挥发性盐基氮）；3）建立了南极磷虾粉品质评价指标的分级标准和评分标准；4）建立了南极磷虾粉品质判别函数，判别率较高。该研究构建的南极磷虾粉品质评价体系为南极磷虾粉品质评价提供了科学依据，可以用于南极磷虾粉的品质定性判别和评价。     

基于多元变量的南极磷虾拖网作业状态影响因素分析

拖网作业过程中的网位、网身状态、拖网整体状态是评价拖网性能优劣的重要指标。以南极磷虾拖网为例，通过测量拖网不同部位（上纲、第3~4节网身连接处和网囊口上部中点）深度，以部位间深度差表示拖网作业状态，分析捕捞操作、海洋环境和渔获量对拖网作业状态的影响，确定中层拖网作业过程中状态的变化趋势。结果表明：（1）上纲与第3~4节网身连接处深度差范围为-0.20~8.02 m，上纲与网囊口上部中点深度差范围为6.49~30.16 m；（2）曳纲长度、拖速、风速、150 m水层流速对磷虾拖网上纲深度影响显著（P< 0.05）；上纲深度与曳纲长度和150 m水层流速呈正相关关系，与拖速呈负相关关系，随风速增加，上纲深度先减小后增大；（3）拖速、200 m水层流速和浪高对上纲与第3~4节网身连接处深度差影响极显著（P< 0.05）；上纲与第3~4节网身连接处深度差具有随拖速和200 m水层流速的增加，先增大后减小的趋势；其中拖速2.6 kn和200 m水层流速0.3 kn时，上纲与网囊口上部中点深度差最大，2 m浪高时，深度差最小；（4）渔获量与上纲和网囊口上部中点深度差呈正相关关系；（5）曳纲长度是影响拖网作业状态的最重要因素，其次是拖网速度、风速、浪高和水流速度。     

不同配比南极磷虾粉饲料对网箱养殖俄罗斯鲟生长及氟累积的影响

配制南极磷虾粉替代饲料中鱼粉比例分别为0%、10%、20%、30%的4组饲料饲喂俄罗斯鲟(Acipenser gueldenstaedtii)200 d后,分析南极磷虾粉替代部分鱼粉对网箱养殖俄罗斯鲟生长和鱼体组织氟残留的影响。结果表明:(1)随着南极磷虾粉替代比例的升高,俄罗斯鲟的末重、增重、特定生长率、蛋白质效率先上升后下降,饲料系数先降低后升高,10%组显著高于对照组和其它试验组(P0.05);(2)实验结束时,俄罗斯鲟肌肉、肝脏和鱼鳔的氟浓度低于检测限,但鳃、皮、脊骨和背骨呈现剂量浓度效应,各试验组较对照组显著升高(P0.05)。综上所述,在本实验条件下,在俄罗斯鲟饲料中加入10%比例的南极磷虾粉时获得最佳生长效果和较低的组织氟累积。     

南极磷虾粉潜在危害因子分析及风险评价进展

南极磷虾粉是目前南极磷虾船载加工的主要产品之一,富含优质蛋白质、脂质、磷脂、虾青素和矿物质等物质,具有良好的开发利用前景。目前,南极磷虾粉主要用作水产动物饲料及提取油脂等的原料。然而,研究表明南极磷虾粉也存在潜在危害因子不明确等问题,严重限制了其应用领域和范围的拓展。因此,本文分析了南极磷虾粉潜在危害因子的来源、限量标准、检测方法等,介绍了南极磷虾粉风险评估进展,旨在为南极磷虾粉危害因子的减除和深度利用提供参考依据。     

Nematode diversity and distribution in the southern maritime Antarctic—clues to history?

Nematode worms are one of the most important soil faunal groups in Antarctica. However, relatively little is known about their wider distribution, biogeography and history in the region, and taxonomic information remains confused or incomplete. Here, we hypothesise that the Alexander Island (southern maritime Antarctic) fauna includes elements that have survived (at least) the period of Pleistocene glaciation in situ, forming a regional centre of endemism and biodiversity hotspot. We describe nematological surveys carried out across a latitudinal gradient between 68 and 77°S along the southern Antarctic Peninsula, comparing the data obtained with the maritime Antarctic fauna described in the few previous studies between northern Marguerite Bay and the South Orkney Islands (60-68°S). In general, our survey supports previous findings of a lack of overlap at species level between the maritime and continental Antarctic biogeographical zones, with the large majority of specimens obtained from all survey sites being attributable to known maritime or new and currently endemic taxa. However, collections from Alexander Island, Alamode Island and the most westerly site sampled, Charcot Island, include specimens morphologically very close to two known continental Antarctic species, which may indicate a link between the two regions. The fauna obtained at the northern study sites (ca. 68°S, Adelaide Island, Marguerite Bay) closely matches that described previously. However, in contrast with widely described patterns of decreasing diversity in other Antarctic biota, species richness increased markedly at locations on Alexander Island (ca. 72°S), including a substantial element of undescribed species (50% of taxa across all locations, 40% of taxa found on Alexander Island). Finally, the most southerly samples obtained, from inland nunataks in Ellsworth Land (75-77°S), indicate a fauna that does not include nematodes, which is exceptional not only in an Antarctic context but also for soils worldwide.     

Modelling local-scale determinants and the probability of microarthropod species occurrence in Antarctic soils

Soil fauna in the extreme conditions of Antarctica consists of a few microinvertebrate species patchily distributed at different spatial scales. Populations of the prostigmatic mite Stereotydeus belli and the collembolan Gressittacantha terranova from northern Victoria Land (Antarctica) were used as models to study the effect of soil properties on microarthropod distributions. In agreement with the general assumption that the development and distribution of life in these ecosystems is mainly controlled by abiotic factors, we found that the probability of occurrence of S. belli depends on soil moisture and texture and on the sampling period (which affects the general availability of water); surprisingly, none of the analysed variables were significantly related to the G. terranova distribution. Based on our results and literature data, we propose a theoretical model that introduces biotic interactions among the major factors driving the local distribution of collembolans in Antarctic terrestrial ecosystems.     

Effects of partial or total replacement of fish meal with freeze‐dried krill (Euphausia superba) on growth and nutrient utilization of juvenile Atlantic cod (Gadus morhua) and Atlantic halibut (Hippoglossus hippoglossus) fed the same practical diets

Studies were conducted with juvenile (28 g) Atlantic cod (Gadus morhua) and halibut (Hippoglossus hippoglossus) fed fish meal (FM) based diets with partial (0%, 25%, 50% and 75%) or total (100%) replacement of FM with freeze‐dried krill (FDK). Addition of dietary FDK significantly stimulated feeding activity in both species and resulted in higher feed consumption and intakes of digestible protein (DP) and energy (DE). This is a significant finding on the feed attractant properties of FDK because it occurred despite having 2–4 MJ kg^?1 higher levels of DE in the FDK diets which could have had an intake suppression effect. In addition, it indicates that the soluble protein fraction of krill thought to be responsible for its attractant properties and is routinely lost or greatly reduced during commercial processing of krill meals, is retained by freeze‐drying. Supplementation of FDK significantly improved growth rates, digestibility and nutrient utilization. Levels exceeding 25–50% FM replacement, however, generally did not significantly increase growth performance or nutrient utilization. In addition, feed formulations with high FDK (>75% replacement) are likely to be cost‐prohibitive as long as the market price remains higher than FM.     

Acoustic identification of isada krill, Euphausia pacifica Hansen, off the Sanriku coast, north-eastern Japan

In this study, the feasibility of identification and abundance estimation of isada krill, Euphausia pacifica , was examined using a two-frequency (38 and 120 kHz) method. The acoustic survey and midwater trawling were conducted off the Sanriku coast, northern part of Honshu, Japan. In one pattern, the SV difference between the two frequencies was large, and in the other, the SV difference was small. These results were interpreted by scattering models, and showed that discrimination between isada krill and walleye pollock was possible.     

Prey selection of common minke (Balaenoptera acutorostrata) and Bryde's (Balaenoptera edeni) whales in the western North Pacific in 2000 and 2001

A study of common minke and Bryde's whales was conducted in the western North Pacific in the 2000 and 2001 summer seasons to estimate prey selection of cetaceans as this is an important parameter in ecosystem models. Whale sighting and sampling surveys and prey surveys using quantitative echosounder and mid‐water trawl were carried out concurrently in the study. Biomasses of Japanese anchovy, walleye pollock and krill, which were major prey species of common minke and Bryde's whales, were estimated using an echosounder. The results suggested that common minke whale showed prey selection for Japanese anchovy while they seemed to avoid krill in both the offshore and coastal regions and walleye pollock in the continental shelf region. Selection for shoaling pelagic fish was similar to that in the eastern North Atlantic. Bryde's whale showed selection for Japanese anchovy in August 2000 and July 2001, while it showed prey selection for krill in May and June in 2001.