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1.
我国油菜根肿病发病面积在66.7万公顷左右,约占总生产面积的10%,已严重威胁到油菜的安全生产。基于此,本研究以含有CRb抗根肿病位点的大白菜材料CR Shinki为供体亲本,以甘蓝型油菜国审杂交种华油杂62的父本Pol.CMS恢复系Bing409为受体亲本,通过杂交、回交及自交等育种程序,结合前景和遗传背景筛选,将CRb抗病位点导入到Bing409中。在BC3F2代获得了遗传背景高度接近Bing409且含CRb抗病位点的抗根肿病新恢复系Bing409R,进而成功选育了我国首个抗根肿病杂交油菜新品种华油杂62R。CRb抗病位点在甘蓝型油菜背景中表现为单基因显性遗传,根肿病抗性遗传改良的同时并未对Bing409R及由其配制的杂交种华油杂62R的产量、品质造成不良影响,Bing409R及华油杂62R对我国四川、湖北、安徽等地区根肿菌生理小种具有免疫抗性。本研究的开展为我国油菜抗根肿病育种提供了宝贵资源,为我国抵抗油菜根肿病的威胁提供了重要保障。  相似文献   
2.
AIM:To investigate the potential role of p62dok in the regulation of hepatic gluconeogenesis. METHODS:The expression of p62dok, insulin signaling transduction, and hepatic gluconeogenesis were investigated in the liver tissues of mice treated with high-fat diet(HFD) and in cultured mouse hepatocytes treated with free fatty acid(FFA). The experiments of gene silencing and overexpression were conducted to observe the effects of p62dok on insulin signal transduction and hepatic gluconeogenesis in cultured mouse hepatocytes. Western blotting was used to detect the protein levels and the phosphorylation statue. RESULTS:The increased p62dok levels were found in the liver tissues from HFD-treated mice and FFA-treated hepatocytes. Meanwhile, phosphorylation of Akt and forkhead box O1 protein(FoxO1) was were decreased and the expression of glucose-6-phosphatase(G6Pase) and phosphoenolpyruvate carboxykinase(PEPCK) was increased. Silencing of p62dok in cultured hepatocytes treated with FFA induced the increase in phosphorylation of Akt and FoxO1, and decrease in the protein levels of G6Pase and PEPCK. CONCLUSION:Up-regulation of p62dok induced by HFD or FFA enhances hepatic gluconeogenesis via inhibiting insulin signal transduction.  相似文献   
3.
N-methyl-D, L-aspartate (NMA) elicited secretion of growth hormone (GH)-releasing hormone from both the hypothalamic-preoptic area and the median eminence that were collected from boars. We suggest that the previously described increase in GH secretion that follows peripheral treatment of swine with NMA is attributable, at least in part, to NMA-stimulated secretion of GH-releasing hormone from the central nervous system.  相似文献   
4.
江优明62是福建省三明市农业科学研究所育成的晚籼中熟三系杂交稻新品种,在福建永安市作晚稻种植表现出高产、转色好、抗性较好等优点。进行产量构成因素相关和通径分析,该品种构成产量的主要因素是单位面积有效穗。其作晚稻种植高产栽培技术要点:主攻单位面积有效穗数、穗粒数,创建理想的高产群体结构,并发挥其内在的大穗优势、提高结实率和千粒重。  相似文献   
5.
本文报道了农抗SH—62产生菌原生质体再生菌株的筛选和再生菌株R—4的发酵规律。试验表明,通过摇瓶的初筛和复筛,从活性比出发菌株要强的202株再生菌株中,找出R—4、R—66和R—72三株活性最强而又稳定的再生菌株。在再生菌株R—4的发酵试验中,选出了该菌株的最佳发酵培养基为1号培养基,并证明这个菌株对溶氧量并不敏感,在偏碱条件下有利于生长和产素,发酵周期为72小时等最适发酵条件。  相似文献   
6.
花生新品种湛油62的选育研究   总被引:3,自引:0,他引:3  
湛油62是湛江市农科所以(湛油30×CS41)F4作母本,梧油6号作父本进行有性杂交选育而成的花生新品种。该品种具有高产、优质、高抗锈病和耐涝等优点。在2000~2001年广东省花生品种区域试验中,平均产量3905.25kg/hm2,比第一对照种汕油523增产10.31%,增产达极显著水平;比第二对照种粤油256增产26.61%,增产达极显著水平。该品种于2003年3月通过广东省农作物品种委员会的审定,适宜于我国南方花生产区推广种植。  相似文献   
7.
Recent studies highlighted the role of autophagy as a cardinal regulatory system for homeostasis and cancer‐related signalling pathways. In this context, the deregulated expression of p62 – Sequestosome1 (p62/SQSTM1) – a protein acting both as an autophagy receptor and signalling hub, has been associated with tumour development and chronic inflammation. Multiple clinical studies test drugs targeting autophagy, and even more research is on the way to clinical trials. However, no comparative investigations have been carried out to identify adequate preclinical models to assess p62‐based medicine. In veterinary oncology the role of p62 in cancer‐related pathways has been largely ignored. We compared p62 sequences in multiple organisms and found that canine p62 significantly diverges from the humans and from other animals sequences. Then, we chart by immunohistochemistry the expression levels of p62 in canine mammary tumours. A total of 66 tumours and 10 non‐neoplastic mammary samples were examined. The expression of p62 was higher in normal tissue and adenomas than carcinomas, with lowest levels of p62 protein detected in high grade carcinomas. In all cases examined the tumour stroma appeared to be p62‐negative. Taken together our results would suggest that in dogs the association between p62 expression and cancer cells overturns that reported in human breast carcinoma, where p62 accumulates in malignant cells as compared to normal epithelium. Thus, at least in canine mammary tumours, p62 should be not considered a tumour‐rejection antigen for an anti‐cancer immunotherapy.  相似文献   
8.
纤维素酶高产菌株的筛选及最适产酶条件   总被引:2,自引:0,他引:2  
从本实验室保存的57株菌株中筛选出一株可高效降解纤维素的纤维素酶高产菌株哈茨木霉APS62,其滤纸酶活力(FPA)为4.981 IU.g-1,是对照里氏木霉模式菌株APS63的3.02倍.产酶条件优化研究的结果表明,以稻草粉为底物,APS62菌株产滤纸酶的最适条件为:培养温度28℃,培养时间3 d,稻草粉与麸皮比5∶0,接种量6%,氮源为NH4NO3(总氮量0.4%),培养基起始pH为5.0,吐温80含量0.1%.此条件下的FPA为6.125 IU.g-1,比优化前提高8.10%.  相似文献   
9.
Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV) ubiquitously expressed (FAU) gene is down-regulated in human prostate, breast and ovarian cancers. Moreover, its dysregulation is associated with poor prognosis in breast cancer. Sponges (Porifera) are animals without tissues which branched off first from the common ancestor of all metazoans. A large majority of genes implicated in human cancers have their homologues in the sponge genome. Our study suggests that FAU gene from the sponge Suberites domuncula reflects characteristics of the FAU gene from the metazoan ancestor, which have changed only slightly during the course of animal evolution. We found pro-apoptotic activity of sponge FAU protein. The same as its human homologue, sponge FAU increases apoptosis in human HEK293T cells. This indicates that the biological functions of FAU, usually associated with “higher” metazoans, particularly in cancer etiology, possess a biochemical background established early in metazoan evolution. The ancestor of all animals possibly possessed FAU protein with the structure and function similar to evolutionarily more recent versions of the protein, even before the appearance of true tissues and the origin of tumors and metastasis. It provides an opportunity to use pre-bilaterian animals as a simpler model for studying complex interactions in human cancerogenesis.  相似文献   
10.
为确定春油菜新品种华油杂62在伊犁河谷春油菜主产区适宜的氮磷钾施用量,试验采用3414最优回归设计,研究氮磷钾不同施用量对华油杂62产量及农艺性状的影响。结果表明:N2P2K2处理的产量最高,达到181 kg/667m~2;其次为N1P2K2处理,产量为178.1 kg/667m~2;氮、磷、钾单因子效应随着施肥量的增加产量呈现先增长后降低趋势,根据一元二次方程模型,求得施N、P2O5、K2O的最高产量施肥量分别为4.76、12.03、3.04 kg/667m~2,最佳经济施肥量分别为4.25、7.38、1.44 kg/667m~2。  相似文献   
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