工业级棉短绒清理回收工艺的改进

为降低棉短绒中的杂质含量，提高棉短绒的质量及收率，对棉短绒清理回收的生产工艺进行了改进，创新连续化多级除杂装备和短绒精细化分离工艺。棉籽清理除杂工艺中，增加了圆筛、棒条机，并且调整了风力清籽机组配置；短绒分离工艺中，增加了五联筛和新型棒条机。改进后的工艺能够提升对毛棉籽的除杂效果，彻底清理剥绒得到的棉短绒中的碎棉仁、棉壳及棉秆等杂质。此外，该工艺还能回收棉短绒下脚料，满足大型生产线的生产要求。     

滇中冷凉山区不同播期反季花椰菜产量效益分析

为摸索滇中高海拔冷凉山区反季节栽培花椰菜的最佳播期以集成高效栽培技术推广应用,于2017—2018年选择海拔2250 m的云南省峨山县塔甸镇大西村地块进行9个播期的2年随机区组试验。结果表明,花椰菜生育期随播期推迟而延长,而花球采收期除播期7月10日外随播期推迟而逐渐增长;花椰菜株高、外叶数、开展度、球高、球径和单球重等农艺性状有随播期延迟呈现先逐渐减小而后又逐渐增大的趋势;莲座期黑腐病和霜霉病的病情指数随着播期的延迟呈现先逐渐升高而后又逐渐下降的趋势;花椰菜小区产量随着播期的延迟呈现先逐渐下降而后又逐渐提高的趋势,播期4月20日和4月30日与其余7个播期产量之间的差异达极显著水平。综合花椰菜在冷凉山区反季节栽培的生产实际和各播期产量产值及商品性表现,推荐滇中高海拔冷凉山区反季节栽培花椰菜的最佳播期为4月20—30日。     

Screening and Verification of Low Nitrogen Tolerant and Nitrogen Sensitive Cotton Germplasm

[Objective] The aim of this study is to evaluate nitrogen efficient cotton germplasms and improve nitrogen use efficiency. [Method] Eighty cotton germplasms were selected and evaluated in the hydroponic experiment under low (0.25 mmol·L^－1) and high (5 mmol·L^－1) nitrogen concentration. Different traits for screening were identified and nitrogen use efficiency types were classified. Field experiments were also performed for comparison and confirmation of the identified germplasms. [Result] The results showed that there were significant differences in the total plant dry matter, shoot nitrogen accumulation and nitrogen absorption efficiency in cotton germplasms at the two nitrogen levels. Based on coefficient of variation, principal component analysis and correlation, six traits including total plant dry matter, shoot dry matter, root dry matter, total nitrogen accumulation, shoot nitrogen accumulation and nitrogen absorption efficiency were used as screening indicators. According to the Heatmap clustering analysis and the nitrogen efficiency comprehensive index, two germplasms (Lu05R59 and CCRI 69) were identified as low nitrogen tolerant and nitrogen efficient, and two germplasms (Coker 201 and Xinluzhong 30) as low nitrogen sensitive and nitrogen inefficient. The results of field experiment were consistent with the results of the hydroponic culture at the seedling stage. [Conclusion] It was finally determined that Lu05R59 and CCRI 69 were the low nitrogen tolerant and nitrogen efficient germplasms, and Coker 201 and Xinluzhong 30 were low nitrogen sensitive and nitrogen inefficient germplasms. The results of these studies provide the possibility for screening and rapid identification of nitrogen use efficiency in cotton at the seedling stage, and provide the ideal materials and theoretical basis for further study of cotton nitrogen efficient.     

丛生竹林下竹荪富硒菌棒覆土栽培技术

在丛生竹林下对不同基质配方和硒浓度菌棒进行埋棒栽培以及覆土方式栽培试验，结果表明，菌棒不同基质配方对竹荪产量及其营养成分有显著影响，而且三列浅沟形和双列龟背形2种覆土方式有利于提高竹荪产量。改良基质配方菌棒竹荪产量比常规配方提高约50%；在添加外源硒质量分数为0~2.0 mg/kg的浓度范围内，竹荪子实体产量先随着硒浓度的增加而逐渐增加后再有所降低，基质中添加1.5 mg/kg硒肥比不添加产量提高了195.30%。基质中添加硒质量分数为1.0~2.0 mg/kg的硒肥可以较显著地提高竹荪花的硒含量，其干物质中硒含量平均值从约2.50 mg/kg提高到8.05~13.30 mg/kg，外源硒肥利用率达到9.70%~15.36%。菌棒不同基质配方对竹荪子实体的粗蛋白及粗多糖含量有明显影响，改良配方竹荪蛋与竹荪花的粗蛋白含量是常规配方的1.21和1.29倍，其粗多糖含量是常规配方的4.81和1.35倍；基质中添加硒肥与不添加硒肥相比，竹荪蛋与竹荪花中粗蛋白含量分别增加了40.90%和14.30%。在硒质量分数为1.0 mg/kg的竹荪菌棒林下覆土栽培试验中，三列浅沟形覆土方式单位面积鲜竹荪蛋产量最高，为10.27 kg/m²；双列龟背形覆土方式单个菌棒的鲜竹荪蛋产量最高，达1.40 kg。三列浅沟形和双列龟背形2种覆土方式基质生物转化率分别为93.00%和94.14%，达到了较高水平。     

雪山草鸡感染传染性法氏囊病病毒新型变异株的鉴定

传染性法氏囊病病毒(infectious bursal disease virus,IBDV)新型变异株正在我国广泛蔓延,给商品肉鸡群和蛋鸡群带来了新的威胁。本研究对一个疑似发生非典型传染性法氏囊病的地方品种雪山草鸡群进行了RT-PCR检测,并对分离的3株毒株进行了基因测序及序列分析。结果显示,引起该鸡群发病的病原是IBDV新型变异株,属于A2dB1基因型;这3个毒株的VP2高变区编码蛋白上含有IBDV变异株的特征性氨基酸,也具有IBDV新型变异株的独特氨基酸。结果提示,我国地方品种鸡群中也有IBDV新型变异株的流行,现有的部分商品化IBDV疫苗已不能有效预防IBDV新型变异株的流行。     

不同吸附特性的稻草生物炭对稻田氨挥发和水稻产量的影响

秸秆生物炭具有改善土壤生态环境、土壤蓄水保肥和减少温室气体排放等正效应,但其石灰效应会加大稻田氨挥发损失。为充分发挥生物炭吸铵特性,降低其石灰效应的不利影响,对不同热解温度(300、500、700℃)和酸化水平(pH值=5、7、9)稻草生物炭处理下的田面水NH_4~+-N浓度、氨挥发和水稻产量进行了研究。结果表明:偏酸性(pH值=5)、中性(p H值=7)生物炭处理在基肥期和分蘖肥期均能显著降低田面水NH_4~+-N峰值浓度(P0.05),降幅达16.90%～35.60%。全生育期稻田氨挥发损失占施氮量的15.14%～26.05%(2019年)、15.10%～19.00%(2020年)。稻田增施热解温度为700℃、酸化水平为5(p H值=5)的生物炭(C700P5)降氨效果最好,两年氨挥发分别显著降低22.93%、12.61%(P0.05)。高温热解配合偏酸性、中性生物炭(C700P5、C700P7)增产效果显著,增产率达9.92%～13.50%,结构方程模型表明,其增产原因是生物炭酸化处理降低了稻草生物炭的石灰效应,而热解温度调整提高了生物炭阳离子交换量(CationExchange Capacity,CEC),进而降低了田面水NH_4~+-N浓度和氨挥发损失,最终提高了水稻地上部氮素积累和水稻产量。研究可揭示不同热解温度和酸化水平制备的生物炭在稻田中的应用潜力,并为稻田合理施用生物炭和减少化肥施用量提供理论依据。     

水肥一体化施肥对山地果园‘油㮈’生长及果实品质的影响

以福建特色水果‘油㮈’为试材,在等肥料成本条件下,进行水肥一体化施肥和常规施肥下‘油㮈’树枝梢生长长度和粗度、果实品质、产量表现及施肥成本的比较研究,以期为山地水肥一体化施肥提供参考。结果表明,水肥一体化施肥可在一定程度上促进当年枝梢长度和粗度的增长,提高果实可溶性糖、可溶性固形物及Vc含量,降低可滴定酸含量,提高果实平均单果重、可食率、产量。等价施肥条件下,水肥一体化施肥节省人工成本1104.0元/hm²。建议结合降水量等气候条件及山地土壤特点、生态环境等对水肥一体化施肥技术进行优化。     

长江中下游地区棉花涝灾渍害预防及灾后管理技术

针对我国长江中下游地区因梅雨季节阴雨时间长、降水量大而集中常常引发涝灾和造成棉花渍害的现象,总结提出了一系列棉花防涝防渍及灾后田间管理技术措施与要求,以期为长江中下游地区棉农提供技术指导,促进当地棉花产业的发展和植棉效益的提高。     

基于GlobeLand 30的哈密市2000-2020年土地利用格局变化研究

[目的]研究新疆维吾尔自治区哈密市近20 a的土地利用变化,为该地区土地利用规划及生态环境保护提供有力支撑。[方法]基于2000,2010,2020年的GlobeLand 30数据集,通过利用转移矩阵、坡度分布指数、标准差椭圆以及土地利用重心迁移,分析哈密市2000-2020年土地利用的时空变化特征。[结果]①哈密市2000-2020年土地利用变化比较明显。2020年各土地利用类型面积比例为:未利用地>草地>耕地>林地>建设用地>水域。哈密市虽然面积大但能利用的绿洲面积较小。2020年草地面积为10226.39 km²,水域面积为295.17 km²。2000-2020年土地利用类型变化量中,草地的减少量最大,流失面积为671.48 km²,主要转换成未利用地、林地和少量耕地;建设用地增量最大,面积高达405.60 km²。2000-2020年其他土地利用类型中林地和耕地面积呈增长趋势;未利用地和水域呈减少趋势,其中水域面积的减少量很小,基本保持稳定。②2000-2020年草地在5个坡度梯度上分布比较均匀;而耕地和建设用地基本分布在Ⅰ级和Ⅱ级坡度梯度,耕地在Ⅰ级坡度梯度上显优势分布;林地在Ⅰ级坡度梯度上面积最大;未利用地分布面积随着坡度梯度的增加而减少。③2010-2020年土地利用变化与2000-2010时段的变化相比方向性较明显,范围更广,重心向西北方向迁移。[结论]2000-2020年哈密市的土地利用变化有着明显的时空特征,坡度对各地类分布的影响较为明显。     

Effects of DHRS3 in C2C12 Myoblast Differentiation and Mouse Skeletal Muscle Injury

Myoblast differentiation is an essential process during skeletal muscle development. C2 C12 myoblast is a commonly used experimental model to study muscle cell differentiation in vitro. Dehydrogenase/reductase(SDR family) member 3(DHRS3) is a highly conserved member in short-chain alcohol dehydrogenase/reductase superfamily and has been shown to be involved in the metabolism of retinol. Previous experimental results showed that the expression of DHRS3 increased significantly during the differentiation of myoblasts differentiation. However, the effect of DHRS3 on mouse muscle cell differentiation was unclear. The objective of current study was to determine if DHRS3 affected muscle cell differentiation, and if DHRS3 was involved in muscle regeneration. Protein expression was determined by western blot and immunofluorescence analysis. The activation and inhibition of DHRS3 increased and decreased C2 C12 myoblast differentiation respectively, which indicated that DHRS3 could affect C2 C12 myoblast differentiation. DHRS3 expression was significantly changed during muscle regeneration, with the regeneration of muscle injury, the expression of DHRS3 tended to increase first and then decrease. It suggested that DHRS3 might be involved in muscle regeneration. In summary, this study confirmed the involvement of DHRS3 in C2 C12 myoblast differentiation and mouse skeletal muscle regeneration and provided a theoretical basis for further elucidating the molecular mechanism of muscle development.