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从湖南省甘薯种植发展趋势入手,剖析了近年来湖南省甘薯产品功能从饱腹鲜食向多样化需求转变、产业布局由边远山区向经济发达地区拓展以及产业发展由单一粗放向规模化优质化转变的发展现状,指出了当前甘薯产业发展过程中存在产业发展缺乏顶层设计、种薯种苗繁育体系空白、基础设施建设滞后、产业化发展程度不高和科技支撑服务能力不足等问题,基于这些问题,提出了加强规划引领、构建现代种业体系、加强基础设施建设、推动全产业链发展、强化科技推广服务、加大政策扶持力度等推进甘薯产业高质量发展的对策. 相似文献
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为评估四川省兽医系统实验室检测能力和水平,2018—2020年四川省动物疫病预防控制中心组织全省21个地市(州)级兽医实验室开展了检测能力比对。结果显示:2018—2020年全省整体平均正确率分别为95.59%、97.09%和97.88%,说明四川省地市(州)级兽医实验室检测能力处于较高水平,能满足非洲猪瘟、禽流感等重大动物疫病检测需要。同时,部分实验室也反映出一些问题,如设备差、检测水平不高、检测报告不规范等。建议加大兽医实验室建设力度,更新完善检测设施设备;加强实验室技术人员培训,提升其检测能力和水平;同时继续推进兽医系统实验室考核工作,加强比对考核。 相似文献
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Yuhua Fu Pengyu Fan Lu Wang Ziqiang Shu Shilin Zhu Siyuan Feng Xinyun Li Xiaotian Qiu Shuhong Zhao Xiaolei Liu 《Journal of animal science》2021,99(2)
Despite the broad variety of available microRNA (miRNA) research tools and methods, their application to the identification, annotation, and target prediction of miRNAs in nonmodel organisms is still limited. In this study, we collected nearly all public sRNA-seq data to improve the annotation for known miRNAs and identify novel miRNAs that have not been annotated in pigs (Sus scrofa). We newly annotated 210 mature sequences in known miRNAs and found that 43 of the known miRNA precursors were problematic due to redundant/missing annotations or incorrect sequences. We also predicted 811 novel miRNAs with high confidence, which was twice the current number of known miRNAs for pigs in miRBase. In addition, we proposed a correlation-based strategy to predict target genes for miRNAs by using a large amount of sRNA-seq and RNA-seq data. We found that the correlation-based strategy provided additional evidence of expression compared with traditional target prediction methods. The correlation-based strategy also identified the regulatory pairs that were controlled by nonbinding sites with a particular pattern, which provided abundant complementarity for studying the mechanism of miRNAs that regulate gene expression. In summary, our study improved the annotation of known miRNAs, identified a large number of novel miRNAs, and predicted target genes for all pig miRNAs by using massive public data. This large data-based strategy is also applicable for other nonmodel organisms with incomplete annotation information. 相似文献
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穗是水稻(Oryza sativa L.)重要的生殖器官,其生长发育直接影响水稻的产量和品质。前期研究中,我们对水稻生殖生长时期穗发育各阶段的转录组、蛋白组数据进行联合分析,筛选到一个MYB基因家族的转录因子OsZ314基因。生物学信息分析表明,该基因位于1号染色体上,拥有典型的R2R3-MYB型转录因子结构域,且在水稻幼穗时期表达量最高;亚细胞定位结果显示,OsZ314基因定位于细胞核中;转录激活试验表明,OsZ314基因具有转录激活功能。本研究为深入探索OsZ314基因在水稻穗发育时期的分子生物学功能奠定了良好的基础。 相似文献
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Yu Lu Loïc Deblais Gireesh Rajashekara Sally A. Miller Yorsa A. Helmy Haijun Zhang Ping Wu Yanhong Qiu Xiulan Xu 《Plant pathology》2020,69(5):818-826
Acidovorax citrulli is a seed-transmitted gram-negative bacterium that can cause substantial economic yield loss in watermelon and melon production worldwide. Four small-molecule libraries containing 4,952 compounds were selected for high-throughput screening against A. citrulli wild-type strain Xu3-14 by evaluation of growth inhibition. One hundred and twenty-seven molecules (2.5% hit rate) were identified as bactericidal or bacteriostatic against A. citrulli at 100 μM. Secondary screens indicated that 27 candidate compounds were more effective against A. citrulli Group II strains than Group I strains (classified using repetitive element PCR). Several compounds were inhibitory to other pathogenic bacteria, including Clavibacter michiganensis subsp. michiganensis and Xanthomonas campestris pv. campestris, but did not affect the growth of plant beneficial bacteria Pseudomonas fluorescens and Bacillus subtilis. More than half of the compounds did not inhibit germination of Arabidopsis or watermelon seeds. The effect of small molecules on A. citrulli seed-to-seedling transmission was evaluated by applying each compound to inoculated watermelon seeds and assessing seedling infection. Nine compounds were chosen for further investigation based on their reduction of percentage seedling infection and compiling scores on their specificity, sensitivity, and phytotoxicity obtained in the secondary screens. The five best compounds were selected (thiamphenicol, nadifloxacin, pipemidic acid, ciclopirox, and zinc pyrithione) for greenhouse tests and were found to effectively reduce the seed-to-seedling transmission of A. citrulli in both artificially and naturally infested seeds. These top five compounds provide a basis for future development of an A. citrulli-specific bactericide. 相似文献
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裘烨真 《国家林业局管理干部学院学报》2018,(2):13-18
通过自然缺失症后果调查及成因分析,探讨加强生态教育、建设生态城镇、治理环境污染、完善生态旅游等途径,以期降低生态退化,加强生态重建,重塑人与自然的亲密关系,实现环境保护与经济发展的双赢。 相似文献
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