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1.
Daisuke Shiode Yasuzumi Fujimori Fuxiang Hu Susumu Shimizu Teisuke Miura 《Fisheries Science》2001,67(1):110-117
SUMMARY: Flume-tank experiments were performed to examine the depth response of a new type of depth-controlled gillnet. Variations of net depth were investigated as the warp was paid out and wound up for different changes of warp length, main sinker weights, and winch speeds. In most experiments, when the warp finished paying out, the net continued to descend and then ascended slightly to an equilibrium depth (overshoot phenomenon). The overshoot distance was nearly constant when the warp was wound up and increased linearly with increasing winch speed when the warp was paid out. An increase in winch speed reduced net settling time, which converged on a constant value for both paying out and winding up. 相似文献
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Youji Wang Menghong Hu Weimin Wang Ling Cao Yi Yang Biping Lü Rongrong Yao 《Aquaculture International》2008,16(6):539-549
The diel feeding rhythm and ontogenesis during early life stage of loach Misgurnus anguillicaudatus were investigated under experimental conditions (light: L 06:00–18:00, D 18:00–06:00 h). Morphological and behavioral developments
of loach from newly hatched to 40 days after hatching were observed. Larvae were able to prey on daphnia 3–4 days after hatching
at 23 ± 0.5°C. As the larvae grew, they showed an increasing feeding capacity and a distinct feeding rhythm. Feeding intensity
and incidence for day-4 larvae were highest at 10:00 and 16:00 h. The highest levels of feeding intensity for day-12 larvae
occurred at 08:00, 12:00, and 18:00 h as did feeding incidence. By day 20, when the larvae metamorphosed, the highest levels
of feeding intensity occurred at 06:00, 18:00, and 24:00 h and were concurrent with the highest feeding incidence. After metamorphosis,
feeding capacity had again increased considerably and, in contrast to the earlier stages before day 20, feeding intensity
for day-30 juveniles peaked at 05:00 and 20:00 h, about 1–2 h after the maximum feeding incidence. The feeding rhythm of loach
juveniles at day 40 was almost the same as the day-30 juveniles. The estimated maximum daily feeding rates were 43.1%, 33.4%,
19.0%, 12.8%, and 5.8% of body weight on days 4, 12, 20, 30, and 40, respectively. Thus, loach was found to have different
feeding rhythms in the pre- and post-metamorphosis stages, with the highest feeding activity in daytime during the larval
planktonic stage before metamorphosis, and intensely nocturnal feeding behavior during the juvenile benthic stage after metamorphosis. 相似文献
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Dvl1及其结构缺失突变体ΔDIX(dsh and axin)和ΔDEP(dsh,Egl-10 and pleckstrin)腺病毒载体的构建并验证其在MSCs(mesenchymal stem cells)中的表达情况。将目的基因定向克隆至pAdTrack-CMV穿梭载体上,并经PmeⅠ线性化后在BJ5183细菌中与pAdEasy-1骨架质粒同源重组,获得重组腺病毒载体,在QBI-293A细胞中包装及扩增,实时荧光定量PCR和Western bolt验证Dvl1在MSCs中的表达情况。经PCR、PacⅠ单酶切鉴定及测序分析,成功构建Ad-Dvl1、Ad-ΔDIX和Ad-ΔDEP腺病毒载体,目的基因序列与GenBank报道一致,并选出150 MOI为最适感染MSCs的感染复数,成功构建了Ad-Dvl1、Ad-ΔDIX和Ad-ΔDEP腺病毒载体,并获得高滴度的病毒子,qPCR和Western blot证实Dvl1在MSCs中高表达并增加了β-catenin在细胞中的累积,为进一步研究Dvl1在MSCs迁移过程的作用奠定了基础。 相似文献
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大学变革的趋势:创业型大学的兴起 总被引:1,自引:0,他引:1
为了应对西方高等教育财政紧缩政策以及大学间日趋激烈竞争的严酷生存环境,西方部分大学通过一系列的组织转型和内部变革,造就了一种全新的大学发展模式:创业型大学。创业型大学的兴起,为当前我国大学的改革和发展提供了有益的启示。 相似文献
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Yu Ma Shi‐ying Bao Tao Yang Jin‐guo Hu Jian‐ping Guan Yu‐hua He Xue‐Jun Wang Yu‐ling Wan Xue‐lian Sun Jun‐ye Jiang Cui‐xiang Gong Xu‐xiao Zong 《Plant Breeding》2013,132(4):397-400
Simple sequence repeat (SSR) marker is a powerful tool for construction of genetic linkage map which can be applied for quantitative trait loci (QTL) and marker‐assisted selection (MAS). In this study, a genetic map of faba bean was constructed with SSR markers using a 129 F2 individuals population derived from the cross of Chinese native variety 91825 (large seed) and K1563 (small seed). By screening 11 551 SSR primers between two parents, 149 primer pairs were detected polymorphic and used for F2 population analysis. This SSR‐based genetic linkage map consisted of 15 linkage groups with 128 SSR. The map encompassed 1587 cM with an average genetic distance of 12.4 cM. The genetic map generated in this study will be beneficial for genetic studies of faba bean for identification of marker‐locus‐trait associations as well as comparative mapping among faba bean, pea and grasspea. 相似文献
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