禅意与共生

日本的传统哲学和传统建筑中包含着深厚的共生理念和“禅”的哲学。二战以后,日本当代建筑师在融合了传统哲学和近现代西方哲学思想的基础上,以不同的方式拓展和表现了这种共生和禅意的生态思想,在当今建筑领域树立了日本建筑的独特风格。结合笔者对中国美术学院的认识,探讨禅意和共生理论。     

A preliminary study on the effects of dietary supplementation of brewers yeast and nucleotides, singularly or in combination, on juvenile red drum (Sciaenops ocellatus)

This study was designed to evaluate the effects of dietary supplementation with brewers yeast (Brewtech^®) and nucleotides (Optimûn), either singularly or in combination, on red drum growth, body composition, stress responses and possible resistance to Amyloodinium ocellatum infection. Juvenile red drum (～1 g initially) fed practical fish‐meal‐based diets for 6 weeks had similar weight gain regardless of whether the diet was unsupplemented (basal) or supplemented with brewers yeast (2% of diet), nucleotides (Optimûn at 0.2% of diet) or both brewers yeast (2% of diet) and nucleotides (Optimûn at 0.2% of diet). Dietary effects on hepatosomatic index, intraperitoneal fat ratio or whole‐body composition were not significant, except that fish fed the singular nucleotide supplement had a significantly higher whole‐body lipid content compared with fish fed the basal diet. No significant dietary effects on cortisol response were observed after a 15 min confinement stress test perhaps because of the extreme variation among individual fish. Subsequently, in situ challenge by co‐habitation with A. ocellatum caused 100% mortality regardless of dietary treatment within a 48 h period. It is concluded that dietary supplementation of brewers yeast and nucleotides did not promote enhanced growth or improved cortisol response and resistance to uncontrolled amyloodiniosis in juvenile red drum, at least at the tested dosages.     

Morphological studies of peripheral blood cells of the Chinese sturgeon, <Emphasis Type="Italic">Acipenser sinensis</Emphasis>

The peripheral blood cells of one-year-old Chinese sturgeon (Acipenser sinensis) have been studied by light microscopy and transmission electron microscopy. The erythrocyte count was 84.86 × 10⁴ cell mm⁻³ in the peripheral blood of the fish and that of leukocytes was 2.24 × 10⁴ cell mm⁻³. The erythrocytes and four main types of leucocyte—thrombocytes, lymphocytes, granulocytes (including neutrophils and eosinophils), and monocytes, were identified in the peripheral blood. In addition to normal erythrocytes, reticulocytes and division of erythrocytes were observed. Thrombocytes were the most numerous among the leukocytes, and the number of neutrophils with lobated nuclei was larger than for other fish. The structures of the erythrocytes, lymphocytes, monocytes, granulocytes, and thrombocytes of the fish were studied. The erythrocytes were almost completely devoid of organelles, except for some mitochondria and granules. A large number of vacuoles and a few organelles were observed in cytoplasm of the monocytes. There were many microvilli on the membrane and pseudopodia-like cytoplasm bulge in the lymphocytes. The neutrophils were round or oval in shape with bilobed, trilobed, or multilobed nuclei whereas the eosinophils had big special granules, dark stained. There were many vesicles in some thrombocytes, which were related to its phagocytosis; some thrombocytes had almost no cytoplasm or organelles.     

Effect of salinity on survival, growth, oxygen consumption and ammonia-N excretion of juvenile whiteleg shrimp, Litopenaeus vannamei

In this study, we tested the lower salinity tolerance of juvenile shrimps (Litopenaeus vannamei) at a relatively low temperature (20 °C). In the first of two laboratory experiments, we first abruptly transferred shrimps (6.91 ± 0.05 g wet weight, mean ± SE) from the rearing salinity (35 000 mg L^?1) to salinities of 5000, 15 000, 25 000, 35 000 (control) and 40 000 mg L^?1 at 20 °C. The survival of L. vannamei juvenile was not affected by salinities from 15 000 to 40 000 mg L^?1 during the 96‐h exposure periods. Shrimps exposed to 5000 mg L^?1 were significantly affected by salinity, with a survival of 12.5% after 96 h. The 24‐, 48‐ and 96‐h lethal salinity for 50% (LS₅₀) were 7020, 8510 and 9540 mg L^?1 respectively. In the second experiment, shrimps (5.47 ± 0.09 g wet weight, mean ± SE) were acclimatized to the different salinity levels (5000, 15 000, 25 000, 35 000 and 40 000 mg L^?1) and then maintained for 30 days at 20 °C. Results showed that the survival was significantly lower at 5000 mg L^?1 than at other salinity levels, but the final wet weight under 5000 mg L^?1 treatment was significantly higher than those under other treatments (P<0.05). Feed intake (FI) of shrimp under 5000 mg L^?1 was significantly lower than those of shrimp under 150 00–40 000 mg L^?1; food conversion efficiency (FCE), however, showed a contrasting change (P<0.05). Furthermore, salinity significantly influenced the oxygen consumption rates, ammonia‐N excretion rates and the O/N ratio of test shrimps (P<0.05). The results obtained in our work provide evidence that L. vannamei juveniles have limited capacity to tolerate salinities <10 000 mg L^?1 at a relatively low temperature (20 °C). Results also show that L. vannamei juvenile can recover from the abrupt salinity change between 15 000 and 40 000 mg L^?1 within 24 h.     

细化设备管理 提高使用效益

随着高等教育事业的发展,国家对高校设备经费投入也逐年增加,但目前的投入远不能满足高等教育事业快速发展的需求。同时,普遍存在的一个情况是,各个高校的设备使用率较低,资源闲置。在有限的资金投入下,如何提高现有设备使用效益,成为最有效的解决这一瓶颈的关键。文章根据云南大学教学科研设备使用情况,结合信息学院目前实验室现有仪器设备及教学任务,探讨了一些高校仪器设备管理的经验和方法。尤其探讨了学校根据实际情况,自主研发了资产管理系统,进一步规范了资产管理工作,提高了设备使用效益,特别是针对大型仪器设备,建立共享平台,实行预约制度,充分体现了大型设备的资源优势。     

KS－1型双功能控湿仪的研制

ＫＳ－１型控湿仪由集成运算放大器、５５５时基电路、固态继电器等先进的电子器件组成，具有双功能、自动控制灵敏、结构简单、使用方便、经济实用等特点。本文介绍了该控湿仪的设计思想、基本原理和性能特点，并对５５５集成芯片组成的单稳、控时电路作了具体的分析     

张履祥与太湖地区农业知识的传播

张履祥农业知识传播与交流的典型耕读士人,他农业知识的来源以及自己农业知识的外传,进一步说明以他为代表的太湖地区底层士人对农业知识传播的贡献。张履祥和其生活范围内人士通过书信等方式的交流,通过这个过程把农业知识介绍给他人。这里介绍了张履祥获得农业知识的方式,包括书籍、实践、老农、家传等途径不断积累自己的农业知识。其次说明了张履祥通过血缘、地缘、结社交游、门人训导的方式将自己总结的农业知识传播出去。这个过程正是一种典型的农业知识传播方式,身为士人的张履祥是农业知识传播的纽带。     

Determination of aflatoxin M1 in milk and milk powder using high-flow solid-phase extraction and liquid chromatography-tandem mass spectrometry

Animal feeds occasionally have some degree of contamination by Aspergillus spp. Even pasteurized milk at times contains the toxic liver carcinogen aflatoxin M1 (AFM1). Confirmation of its presence is now done with solid-phase extraction (SPE) and high-performance liquid chromatography (HPLC)-fluorescence, using a small enough sample that SPE time is reasonable. In this study 200 mL of milk was extracted using a C18 disk at a flow rate of approximately 100 mL/min and AFM1 quantified by HPLC-tandem mass spectrometry with negative electrospray ionization. The effectiveness and cleanup efficacy of immunoaffinity columns (IAC) was compared with that of Mycosep multifunctional cleanup columns (MFC). Average recovery and detection limits of whole milk and low-fat milk cleaned up by IAC were significantly superior to those obtained with the MFC (78-87% and 0.59-0.66 ng/L, respectively). The new procedure improves extraction speed, sensitivity, and specificity.