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排序方式: 共有142条查询结果,搜索用时 156 毫秒
1.
Andrea Arias Aguirre Bruno Studer Javier Do Canto Ursula Frei Thomas Lübberstedt 《Plant Breeding》2014,133(6):765-770
Perennial ryegrass (Lolium perenne L.) displays a two‐locus gametophytic self‐incompatibility (SI) system that remains intact at the tetraploid level. Two models are plausible for SI in autotetraploids. In Model I: both alleles at the S locus and both at the Z locus in diploid pollen matching the female genotype results in incompatibility. In Model II: only one allele at S and one at Z locus in diploid pollen matching the female results in incompatibility. The goals were to determine which of the models best explains SI in our autotetraploid ryegrass population and to evaluate the efficiency of high‐resolution melting (HRM) genotyping for discriminating different iso‐allelic genotypes. The progeny of a cross between two autotetraploids was characterized with three HRM‐based markers co‐segregating with Z. Segregation ratios were used to make inferences about the mode of action of the SI system. The observed segregation differed significantly (P < 0.001) from the expected under Model I, but not from the expected under Model II (P = 0.463). Thus, Model II explains SI in this population, and HRM is an efficient tool to distinguish different iso‐allelic genotypic classes. 相似文献
2.
Tengku Mohamad Tengku-Rozaina Wong Shu Jeng Mat Amin Amiza 《Journal Of Aquatic Food Product Technology》2018,27(6):667-679
Fish is a nutritious food for the whole family and a good source of protein and n-3 fatty acids. The fish-processing industry produces tonnes of fish-processing waste and by-products annually. Disposal of untreated by-products of the fish-processing industry may cause environmental pollution. Sardinella gibbosa or goldstripe sardinella is commonly used in surimi and fish cracker productions in Malaysia. However, data on the nutritional composition of goldstripe sardinella fillets (GSF) and by-products (GSB) remain limited. The objectives of this study are to determine the nutritional composition and thermal properties of fillets and by-products of S. gibbosa. Results showed that the protein and moisture contents of GSF were higher than GSB. Conversely, the ash and fat levels of GSF were lower than GSB. Levels of saturated fatty acids (SFA) in GSF and GSB were higher than monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids. Palmitic acid and oleic acid were the major SFA and MUFA found in GSF and GSB. Both GSF and GSB contain higher percentages of DHA and EPA. Melting and crystallization profiles of the oil from S. gibbosa fillets were similar to by-product oil, which was related to their fatty acid content. 相似文献
3.
本研究在前期构建的刺参(Apostichopus japonicus)高密度遗传连锁图谱和QTL分析的基础上,筛选出26个与体长、体重、体宽、棘刺总数、抗病力相关的SNP位点,设计出可用于HRM检测的SNP扩增引物13对。在扩大群体中利用HRM小片段法对这13个刺参重要经济性状相关的候选SNP位点进行分型和多态性检测,并结合扩大群体的相关性状数据进行了QTL位点验证。多态性结果显示,13个位点中有3个单态性位点,其余10个多态性位点中有3个位点为低等位多态性,7个位点为中等位多态性。10个多态性位点的最小等位基因频率(MAF)介于0.016(SNP113)~ 0.332(SNP160)之间,平均值为0.173;各位点的观测杂合度(Ho)介于0.031(SNP113)~0.818(SNP9)之间,平均值为0.433;期望杂合度(He)介于0.031(SNP113)~0.834(SNP9)之间,平均值为0.402;多态信息含量(PIC)介于0.030(SNP113)~0.393(SNP160)之间,平均为0.248,有6个位点偏离Hardy- Weinberg平衡。QTL验证结果表明,SNP40和SNP160位点为与生长(体长、体重、体宽)相关的位点,各位点的优势基因型分别为SNP40(CC)和SNP160(AA);SNP88、SNP112和SNP126这3个位点为与抗病力相关的位点,各位点的优势基因型为SNP88(CC)、SNP112(AA)和SNP126(TT)。基于这5个位点构建生长和抗病二倍型,发现二倍型K1(CC AA TT)抗病力最强,S1(CC AA)、S3(CC AC)在生长方面优势显著,相关研究结果可为分子标记辅助育种在生产中应用提供基础数据。 相似文献
4.
[目的]探讨融雪时间对川贝母生长和物候的影响。[方法]在青藏高原东部的一个高山雪床,在积雪早融部位与积雪晚融部位之间进行川贝母移植试验,观察川贝母的个体特征和物候的变化。[结果]川贝母的株高、单叶面积和地上生物量在早融与晚融部位之间有显著的差异,且晚融部位的值要大于早融部位的值;融雪时间对川贝母早期和中期物候阶段(如开始生长期、始花期)有影响,而对后期特别是晚期的物候阶段(如植株枯黄期)影响很小。[结论]积雪晚融的环境更有利于川贝母的生长,在人工种植中应遵循这一规律。 相似文献
5.
Wheat flour replacement from 0 to 40% by single tef flours from three Ethiopian varieties DZ-01-99 (brown grain tef), DZ-Cr-37 (white grain tef) and DZ-Cr-387 (Quncho, white grain tef) yielded a technologically viable ciabatta type composite bread with acceptable sensory properties and enhanced nutritional value, as compared to 100% refined wheat flour. Incorporation of tef flour from 30% to 40% imparted discreet negative effects in terms of decreased loaf volume and crumb resilience, and increase of crumb hardness in brown tef blended breads. Increment of crumb hardness on aging was in general much lower in tef blended breads compared to wheat bread counterparts, revealing slower firming kinetics, especially for brown tef blended breads. Blended breads with 40% white tef exhibited similar extent and variable rate of retrogradation kinetics along storage, while brown tef-blended breads retrograded slower but in higher extent than control wheat flour breads. Breads that contains 40% tef grain flour were found to contain five folds (DZ-01-99, DZ-Cr-387) to 10 folds (DZ-Cr-37) Fe, three folds Mn, twice Cu, Zn and Mg, and 1.5 times Ca, K, and P contents as compared to the contents found in 100% refined wheat grain flour breads. In addition, suitable dietary trends for lower rapidly digestible starch and starch digestion rate index were met from tef grain flour fortified breads. 相似文献
6.
Phenotypic and genetic characterization of Pseudomonas syringae strains associated with the recent citrus bacterial blast and bacterial black pit epidemics in Tunisia 下载免费PDF全文
E. Abdellatif M. Kałużna J. D. Janse P. Sobiczewski F. Helali J. R. Lamichhane A. Rhouma 《Plant pathology》2017,66(7):1081-1093
In the spring of 2012, symptoms of a disease resembling citrus blast and citrus black pit were observed in some orchards in Tunisia. The epidemic spread rapidly in the following years. Twenty‐four commercial citrus orchards from four Tunisian regions showing characteristic symptoms of bacterial diseases were surveyed during a 3‐year study. Eighty‐eight Pseudomonas‐like bacterial isolates were successfully obtained from the northeast and west of Tunisia. No isolates were recovered from the central region. Overall, 46 isolates were identified as Pseudomonas syringae pv. syringae and most of them showed similar phenotypic and genetic profiles. The virulence of three selected isolates differed from one plant cultivar to another as well as from the type of plant organ used for the inoculation. In a bioassay test, all isolates produced syringomycin, which was confirmed by molecular detection based on the syrB and syrD genes. Only EC122 possessed syrD but not syrB. DNA fingerprints, based on repetitive sequence‐based polymerase chain reaction (rep‐PCR) and PCR melting profile (PCR MP), were used to determine the potential genetic diversity among strains. Clustering of PCR MP fingerprinting data matched with rep‐PCR fingerprinting data. The generated distribution tree showed that Tunisian isolates were closely related to the citrus reference strain LMG5496. In contrast, EC112, isolated from citrus, and the almond isolate EC122 were distantly related to the type strain LMG1247T isolated from lilac. Such studies have not been reported until now for P. syringae from citrus. 相似文献
7.
ZHAXI Zhuo-ma GUO Xian YAN Ping WU Xiao-yun WANG Hong-bo LIU Jian YANG An-juan ZHANG De-hu WANG Zhao-zhong WANG Cheng-lin WANMA Dan-zhi NUO Ke-jia YANG Ying-chuan 《中国畜牧兽医》2013,(7):84-88
Lipoprotein lipase (LPL) is a 54 kb glycoprotein, and its activity is affected by nutritional factors and hormone levels ect conciliation. Because the particularity of the yak living environment intended Tianjun yak the LPL-Exon7 gene polymorphism at the molecular level, and with the growth and development traits associated analysis for the yak nutritional regulation of species resources protection, genetic breeding scientific theory basis. Qinghai Haixi Tianjun yak anticoagulant 106 extracted blood samples were taken DNA, and polymorphism analysis of candidate genes LPL-Exon7 adopted the use of PCR technology and high-resolution melting curve analysis, and measured yak weight, body length, body height, bust, tube Wai growth traits and genetic effects. The results showed that the polymorphic yak and wild blood yak of the LPL-Exon7 gene were polymorphism, there were three genotypes, named AA, AB, BB, controlled by a pair of alleles A and B, A allele was the dominant allele. Loci balanced detection χ2 test, in line with the law of Hardy-Weinberg, a state of equilibrium (P>0.05). The female and male of domestic yak and wild blood yak polymorphic information were 0.3588 and 0.2103, 0.3219 and 0.3343, polymorphic information content of domestic yak cows was PIC<0.25, at the low degree of polymorphism, the polymorphic information content of domestic yak bull wild blood yak public was in the 0.250.05). Therefore, the LPL-Exon7 was considered as one of yak genetic markers for marker-assisted selection. 相似文献
8.
9.
Dong Lingyan Yang Xuchao Liu Qingcai Zhang Binghuai 《保鲜与加工》1999,(2):54-59
The successive approaching and rapid cooling method is applied to determine the terminal point of melting,the melting rate of pre reducted pellet is carefully measured.Various factors influencing melting rate are studied via orthogonal experiment,and the influences of main factors are carefully studied via the single factor experiment.The apparent activation energy is expounded and calculated. 相似文献
10.
黄丽娜 《农业图书情报学刊》2007,19(5):76-79
从学校公共关系角度,揭示了高校图书馆服务礼仪对图书馆形象和高校形象所具有的形象外显和形象隐喻价值,论述了“规范的服务礼仪”的基本要求,探讨了在服务礼仪的教化和培训中内化和外化相结合的方式。 相似文献