天然植物生长调节剂芸苔素的生物活性及应用浅析

天然芸苔素属于甾体类植物内源生物活性物质,其骨架为甾醇,其中芸苔素内酯生物活性最强,被称为第六类植物激素,是高效、广谱、无毒的植物生长调节剂(PGR)。为更好地将芸苔素推广应用于农业生产,本文概括了芸苔素内酯的生物活性及在粮食、果蔬等作物上的应用。探讨了芸苔素内酯在提高种子活力,促进作物生长,大幅度促进产量提升以及品质改善,提升作物抗逆性,缓解农药对作物影响的研究概况及取得的进展,指出了芸苔素内酯的应用是作物增产增收的关键途径,同时,能够减少化肥、农药的施用量,减少作物种植成本与减轻环境污染,将带来显著的经济效益。     

The Identification of QTL Associated with Cotton Fruit Branch Length Suitable for Mechanized Harvest Utilizing BSA-seq

[Objective] This study aims to identify quantitative trait loci (QTLs) associated with cotton fruit branch length by using the combination of bulked-segregant analysis (BSA) and next-generation sequencing (NGS) method. [Method] In this study, the cotton F₂ segregating population was developed with the short branch line Sujimian 125 and long-branch variety Sikang 1 as cross parents. According to the average internode length of fruit branches in middle, extreme individuals in F₂ population were divided into two groups to generate the bulked DNA samples. The whole-genome resequencing of two DNA bulks was applied to analyze the single nucleotide polymorphism (SNP) and insertion-deletion (InDel) between two groups, and the fruit branch internode length related QTLs were detected using Euclidean distance (ED). [Result] With SNP data obtained from sequencing, a 0.8 Mbp range associated region on chromosome A3 was identified; with InDel data obtained from sequencing, a 1.09 Mbp range associated region on chromosome A3 was identified. Two regions overlapped, and the overlapped range was 0.77 Mbp. [Conclusion] This result suggested that the difference between I-type fruit branch and zero-type was due to different genetic locus and pattern rather than dosage effect of the same gene.     

基于GIS的福建省将乐县土壤侵蚀及保持定量分析

【目的】对福建省将乐县土壤侵蚀及保持量现状进行定量分析,为该地区制定森林经营方案及水土保持工作提供依据。【方法】以福建省将乐县为研究区,首先建立降雨侵蚀力因子(R)图层、土壤可蚀性因子(K)图层、坡长坡度因子(LS)图层,然后依据实际土地覆盖类型和植被覆盖度构建植被覆盖或作物管理因子(C)图层和水土保持措施因子(P)图层。基于地理信息系统(GIS)和修正的土壤通用侵蚀方程(RUSLE)估算土壤侵蚀量和保持量,定量分析坡度(0°,90°)、土地覆盖类型与土壤侵蚀强度之间的相关关系。【结果】2015年研究区的现实侵蚀量为42.64×10~4t/年,平均土壤侵蚀模数为189.07t/(km~2·年)。从6种土壤侵蚀类型来看,微度侵蚀面积占研究区总面积的88.31%,轻度侵蚀面积占10.36%,中度侵蚀面积占1.13%,这3种侵蚀类型的侵蚀量占侵蚀总量的比例分别为12.43%,60.44%和19.79%;强度、极强度、剧烈侵蚀面积及侵蚀量所占比例均较小。各侵蚀等级在研究区内的空间分布较为均匀,总体来说中部、北部地区侵蚀量较大。7个坡度带中,土壤侵蚀量所占比例较大的是[15°,25°)、[8°,15°)和[25°,35°)坡度带,其面积所占比例分别为35.63%,23.95%和17.53%,侵蚀量所占比例分别为44.68%,24.63%和20.90%。研究区年均土壤保持量为6.98×10~6 t,每km~2土壤保持量为3 099.36t/年;有林地和稀疏植被的每km~2土壤保持量均较高,分别为3 773.58和22 254.39t/年,明显高于其他土地覆盖类型。【结论】将乐县微度侵蚀面积所占比例最大,轻度侵蚀的侵蚀量所占比例最大;森林和稀疏植被对将乐县的土壤保持量贡献最大,进行皆伐作业设计时应该尽量减小皆伐面积。     

Preparation and Characterization of Microcrystalline Cellulose from Sacred Bali Bamboo as Reinforcing Filler in Seaweed-based Composite Film

Microcrystalline cellulose (MCC) isolated from cheap, fast-growing and abundant accessible Sacred Bali bamboo (Schizostachyum brachycladum) was utilized as reinforcement material in the seaweed-based composite film. Isolation of MCC was carried out by using a combination of pulping, bleaching and acid hydrolysis process. This study emphasized on the feasibility of MCC production from Sacred Bali bamboo by studying its properties using X-ray diffraction (XRD), scanning electron microscopy (SEM), fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA). The commercial-MCC (CMCC) was used as reference material. Results showed that the production yield, moisture content (MC) and fiber length of bamboo-MCC (BMCC) were 83.37±1.48 %, 4.50±0.5 %, and 0.47±0.02 mm, respectively. According to the chemical analysis by FTIR, both lignin and hemicellulose were completely removed from BMCC, and thus, improved the crystallinity (78 %) and thermal stability (≈325 °C) of BMCC. This study also revealed that MCC produced from Sacred Bali bamboo demonstrated strong mechanical reinforcement effect in the seaweed-based film. Hence, Sacred Bali bamboo-MCC could be used as reinforcement material in the polymer.     

面向数控加工系统的3D打印切片算法与分区扫描策略

为实现大型农机零部件的快速修复与更换,该文针对3D打印协同数控成形大型零件中的折线拐点金属过堆积、工件翘曲变形、层厚难以实时调整等问题,提出数控系统与电子束成形相结合的3D打印切片算法与分区扫描策略。为提高切片速度,实现成形过程中的层厚实时调整,采用反向光线追踪算法对三维模型渲染切片,通过MS(marching squares)算法提取二值图像的坐标序列,快速获取指定层的轮廓坐标;为避免成形过程中电子枪运行速度不均匀引起的金属过堆积现象,选用B样条基函数对轮廓数据进行曲线插值,结合数控系统的曲线插补命令,实现恒定线速度成形。针对大型零件在成形过程中的变形问题,采用六边形分区与平行线变角度扫描技术,根据各分区图案的形心欧式距离规划扫描顺序,实现变形控制。结果表明:采用非均匀有理B样条曲线和直线分段插值后,拟合曲线对原始多重曲面截线的逼近误差范围与切片数据相比减少了30%。选用网格数量为1 483 132的STL(stereolithography)模型进行效率测试,该算法切片用时90 s,与商用软件Magics15.01切片相比用时减少了34.6%,与开源软件Cura15.06切片相比用时减少了31.4%,研究结果可为大型零件成形过程中的层厚动态调整及变形控制等提供新的思路。     

砾石对石灰土表面干缩裂隙发育特征的影响

[目的]研究砾石粒径及含量对石灰土表面干缩裂隙发育特征的影响,为探索喀斯特地区的水土流失机理提供参考依据。[方法]通过室内模拟试验和数字图像处理技术,研究了砾石粒径(2.0～5.0 mm和5.0～12.5 mm)和砾石含量(0%,10%,20%,30%,40%)条件下的石灰土表面干缩裂隙特征。[结果]①无砾石石灰土平均表面裂隙率仅为3.03%,含砾石石灰土具有更大的表面裂隙率,在大粒径(5.0～12.5 mm)高含量(40%)条件下,表面裂隙率最大,达到8.66%;②当砾石含量增加时,裂隙的形态变得细小且密集,小粒径砾石会使裂隙网络更复杂;③大粒径(5.0～12.5 mm)条件下的土壤表面裂隙率与砾石含量成正线性相关,而小粒径(2.0～5.0 mm)条件下的裂隙率与砾石含量成负线性相关;④砾石会成为裂隙发育的基点,每个砾石颗粒周围都有可能产生围绕砾石或是向外延伸的裂隙。且在砾石形态的棱角处,容易产生向外延伸的裂隙。[结论]石灰土中存在砾石会导致表面裂隙率提高,弱化土体的抗侵蚀能力,是石漠化治理不容忽视的问题。     

Influence of in-house produced biochars on cracks and retained water during drying-wetting cycles: comparison between conventional plant,animal, and nano-biochars

Purpose

Biochars produced from different feedstocks (such as wood, pig manure) possess varying physical and chemical properties, which have influence on crack and evaporation rate of biochar-amended soil (BAS). Furthermore, influence of compaction state and drying-wetting cycles on evaporation rate and cracking of BAS has not been investigated comprehensively. The objective of this study was to investigate the effects of biochar types, compaction state of BAS, and drying-wetting cycles on crack propagation and retained water (or evaporation rate).

Material and methods

An animal and plant feedstock-based biochars were produced in-house from pig manure (PM) and wood (W), respectively. In addition, nano structured chalk and wheat biochar (CWB) were also produced. Soil amended with individual biochars was compacted in petri-glass discs at two densities. Disc specimens were subjected to multiple drying-wetting cycles, and evaporation rate of specimens and crack area were monitored throughout the experimental period (70 days). Images were captured after every 24 h and processed using image processing technique to obtain the crack intensity factor (CIF).

Results and discussion

The results show that plant-based W BAS showed the high water retention, i.e., low evaporation rate and low CIF. Furthermore, the crack potential of CW BAS was seen to be higher. In dense compacted soil, maximum CIF% can be reduced from 3.9 to 0.4% for W BAS, from 3.9 to 1.7% for PM BAS, and from 3.9 to 1.6% for CW BAS.

Conclusion

WB was able to resist cracking more efficiently than other types of biochar. Evaporation was found to be minimal for plant-based W BAS at 10% biochar percentage. Higher biochar content in soil was seen to increase the water retention of BAS significantly. Dense state of BAS at high biochar content (i.e., 10%) was effective in reducing evaporation rate and crack progression.

     

低温胁迫对冰菜转录组水平响应分析

利用二代高通量测序技术对低温胁迫处理的冰菜进行测序,构建冰菜转录组数据库.分别得到24.13 Gb有效数据和24045条Unigene的注释,得到DEGs 1902个(T0 vs T1)和2134个(T0 vs T2).T0 vs T1组和T0 vs T2组分别有40和41个功能小类化归GO数据库;分别有20和24个功能分类注释到KOG数据库;155和272条基因注释到KEGG数据库,并分别富集在74和105条代谢通路.T0 vs T1组DEGs主要注释到植物信号转导等4个代谢通路;T0 vs T2组DEGs注释到苯丙醇类生物合成等11个代谢通路,其中正向影响代谢途径:丙酮醇类生物合成、嘌呤代谢、谷胱甘肽代谢、脂肪酸代谢、类黄酮代谢、氨基酸的生物合成代谢途径等;负向影响代谢途径:植物-病原互作、植物激素信号转导、淀粉和蔗糖代谢等途径.通过对淀粉和蔗糖代谢途径关键基因分析表明:低温胁迫1 h (T1),海藻糖6-磷酸合酶、海藻糖-6-磷酸酯酶、β-淀粉酶、葡萄糖-1-磷酸腺苷酰转移酶、糖原磷酸化酶等5个关键基因表现为上调表达,未见下调表达基因;低温胁迫36 h (T2),海藻糖-6-磷酸合酶、己糖激酶、β-淀粉酶等3个关键基因上调表达,葡萄糖内酯-1,3-β-葡萄糖苷酶基因下调表达.选取淀粉和蔗糖代谢途径中8个DEGs,经RT-qPCR分析,8个DEGs的相对表达量与转录组表达水平相符.     

江汉平原中山杉引种造林试验研究

通过在江汉平原开展中山杉6个品系的引种造林试验。结果表明,引进的6个品系中山杉无严重病虫害发生,各生长指标均大于池杉,11 a生中山杉平均胸径为17.61 cm、树高为11.06 m、材积为0.1297 m^3,表明中山杉在江汉平原的引种试验基本成功,其中中山杉502和中山杉118最为突出,适合在江汉平原推广造林,丰富江汉平原造林树种。