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排序方式: 共有1345条查询结果,搜索用时 15 毫秒
1.
    
This study evaluated the effects of dietary concentrate levels and 2‐methylbutyrate (2MB ) supplementation on performance, ruminal fermentation, bacteria abundance, microbial enzyme activity and urinary excretion of purine derivatives (PD ) in steers. Eight ruminally cannulated Simmental steers (12 months of age; 389 ± 3.7 kg of body weight) were used in a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement. Moderate‐concentrate (400 g/kg diet [MC ]) or high‐concentrate (600 g/kg diet [HC ]) diets were fed with or without 2MB (0 g/day [2MB ?] or 15.0 g/day [2MB +]). Dry matter intake and average daily gain increased, but feed conversion ratio decreased with the HC diet or 2MB supplementation. Ruminal pH decreased, but total volatile fatty acid increased with the HC diet or 2MB supplementation. Molar proportion of acetate and acetate‐to‐propionate ratio decreased with the HC diet, but increased with 2MB supplementation. Propionate molar proportion and ruminal NH 3‐N content increased with the HC diet, but decreased with 2MB supplementation. Neutral detergent fibre degradability decreased with the HC diet, but increased with 2MB supplementation. Crude protein degradability increased with the HC diet or 2MB supplementation. Abundance of Ruminococcus albus , Ruminococcus flavefaciens , Fibrobacter succinogenes and Bufyrivibrio fibrisolvens as well as activities of carboxymethyl cellulase, cellobiase, xylanase and pectinase decreased with the HC diet, but increased with 2MB supplementation. However, abundance of Prevotella ruminicola and Ruminobacter amylophilus as well as activities of α‐amylase and protease increased with the HC diet or 2MB supplementation. Total PD excretion also increased with the HC diet or 2MB supplementation. The results suggested that growth performance, ruminal fermentation, CP degradability and total PD excretion increased with increasing dietary concentrate level from 40% to 60% or 2MB supplementation. The observed diet × 2MB interaction indicated that supplementation of 2MB was more efficacious for improving growth performance, ruminal fermentation and total PD excretion with promoted ruminal bacteria abundance and enzyme activity in the MC diet than in the HC diet.  相似文献   
2.
采用随机区组设计,试验了氮、磷和钾水平不同组合的复合肥配方系列及尿素对赤水河流域毛竹林生产力的影响。结果表明:①不同处理对毛竹林出笋成竹量和成竹率、新竹平均胸径和胸高断面积、以及竹秆、枝叶等地上生物量有增产效应,差异不显著。综合效应指数排序为复合肥F (271.34%)>尿素(228.33%)>复合肥G (160.46%)>对照(100.00%)。②复合肥F的出笋量、成竹量、新竹平均胸径、胸高断面积,秆材量和枝叶生物量分别为对照的110.53%、107.22%、103.61%、113.62%、114.93%和109.70%。除出笋量增幅仅中下水平外,成竹量、新竹质量和生物量增幅较大,适宜于毛竹林大径材培育。③复合肥G和尿素的出笋成竹量增幅较大,复合肥G的新竹质量和生物量增幅较小,不及尿素的中上水平,前者可作为笋用林和笋材兼用林培育肥种、肥效较低,后者肥效应较高、不利于地力维护,生产上宜慎用。  相似文献   
3.
从呼和浩特市郊区分离1株兔出血症病毒,命名NM株,对其进行了病毒理化、生物学特性检测,并研究了应用SPA协同凝集试验诊断兔出血症的方法。结果表明,病毒在兔体连续传6代,均出现典型症状及病理变化,是1株毒力稳定的强毒株,病毒粒子在电镜下呈球形,直径30.3nm,无囊膜,边缘有均匀排列的齿状突起,有实芯(占40.6%)和空芯(占59.4%)2种病毒粒子。还有少量形态不一、大小不等的类似病毒的粒子。用冷酚法提取的病毒核酸,经二苯胺显色及紫外扫描,证明为DNA。病毒的蔗糖浮密度为1.192g/ml,对氯仿、乙醚及胰蛋白酶不敏感,耐酸(pH5、pH3)耐热(50℃、56℃、60min)。病毒对人的各型红细胞有高度凝集性。经氯仿、乙醚、酸(pHs、pH3),热(50℃、56℃ 60min)处理后血凝价不降或稍降,经胰蛋白酶处理后血凝价明显降低(降5个滴度)。37℃放置4h,4℃冰箱放置48h血凝价不降,4℃放置4~13d降低一个滴度,15d降2个滴度。兔体血清HI抗体滴度与保护力呈正相关,1∶40以上可以完全抵抗强毒攻击。用内蒙古生物制药厂生产的兔出血症组织灭活苗免疫兔,可以抵抗强毒的攻击。采用原代乳兔肾细胞、Vero及BHK_(21)传代细胞,培养病毒均未成功。应用SPA协同凝集试验诊断兔出血症,特异性和可靠性均好,可作为本病的1种快速诊断方法。  相似文献   
4.
以木薯品种“GR911”、“面包木薯”试管苗叶片为材料,研究木薯枯萎病病原菌粗提物对愈伤组织增殖与分化的影响,以期建立枯萎病病原菌粗提物为选择压力的木薯离体抗病筛选体系。结果表明:所用品种幼叶愈伤诱导与增殖的适宜配方为改良的MS培养基+0.5-0.75 mg/L 2,4-D;愈伤分化适宜配方为的MS培养基+0.75-1.0 mg/ L ZT。“GR911”与“面包木薯”愈伤增殖过程中,愈伤褐化率达到50 %所需的细菌液粗提物浓度分别为5.5×106、2.5×106 cfu /mL;愈伤组织分化过程中,愈伤褐化率达到50 %所需的菌液粗提物浓度分别为7.0×106、4.0×106 cfu /mL。通过建立木薯愈伤组织离体抗病筛选体系,为木薯无性系变异育种奠定基础。  相似文献   
5.
河南省日本落叶松适生区研究   总被引:2,自引:0,他引:2  
根据影响日本落叶松生长的主要因子 ,用灰色关联分析、数量化 -灰色关联分析以及多项式回归等方法 ,对河南省日本落叶松适生区域进行了研究 .结果表明 :河南省适宜日本落叶松生长的海拔范围为 770~ 2 32 0m .其中 ,最适生区海拔为 [1 0 30 ,1 6 5 0 ]m ;适生区海拔为 (1 6 5 0 ,2 32 0 ]m ;次适生区海拔为 [770 ,1 0 30 )m .影响日本落叶松生长的立地因子大小依次为 :海拔、坡向、土层厚度、土壤种类和坡位 ;气象因子大小依次为 :降水量、相对湿度、日照时数、7月份平均气温、无霜期、蒸发量、年均气温、≥ 1 0℃积温、1月份气温  相似文献   
6.
近几年,多数糖厂和用糖户都很重视白糖中的二氧化硫残留量.检查结果说明,有一些糖厂生产的白糖的残留二氧化硫量经常偏高.有些糖厂的白糖,多数质量指标都达到一级糖的标准,就是由于残留二氧化硫超过40mg/kg而成为二级糖.如果按照将要实行的新质量标准(二氧化硫低于30mg/kg),则这个问题会更为普遍.  相似文献   
7.
为了研究赖氨酸成分对紫丁香蘑菌丝体生长的影响,采用不同浓度的赖氨酸-PDA培养基进行紫丁香蘑菌丝体生长的对比试验,确定最适合紫丁香蘑菌丝生长的赖氨酸浓度。结果表明,紫丁香蘑菌丝生长的最适赖氨酸浓度分别为30μg/mL和90μg/mL,此项研究为紫丁香蘑的规范化生长提供帮助。  相似文献   
8.
9.
    
Flexible marine natural products (MNPs), such as eribulin and bryostatin, play an important role in the development of modern marine drugs. However, due to the multiple chiral centers and geometrical uncertainty of flexible systems, configuration determinations of flexible MNPs face great challenges, which, in turn, have led to obstacles in druggability research. To resolve this issue, the comprehensive use of multiple methods is necessary. Additionally, configuration assignment methods, such as X-ray single-crystal diffraction (crystalline derivatives, crystallization chaperones, and crystalline sponges), NMR-based methods (JBCA and Mosher’s method), circular dichroism-based methods (ECCD and ICD), quantum computational chemistry-based methods (NMR calculations, ECD calculations, and VCD calculations), and chemical transformation-based methods should be summarized. This paper reviews the basic principles, characteristics, and applicability of the methods mentioned above as well as application examples to broaden the research and applications of these methods and to provide a reference for the configuration determinations of flexible MNPs.  相似文献   
10.
The present study addresses the mechanism of hydroxyl radical (·OH) generation by the collapse of microbubbles in water solution. The influence of gas supply and flow rate, solution pH, and ionic strength on the aeration efficiency, free radical generation, and contaminant removal (take methylene blue as an example) are elucidated. The results showed that the degradation rate of methylene blue by ·OH increased with flow rate as well as in acidic or alkaline solutions compared to that in neutral conditions. ·OH was shown to be produced by the reaction between protons and oxygen radicals generated by the decomposition of O2 rather than water molecules. A greater concentration of O2 or H+ thus promoted the reaction, resulting in effective removal at a high flow rate or low pH. Nevertheless, there was considerable methylene blue removal at high pH, driven by the production of the dye cation through the dissociation of methylene blue and the high electronegativity of bubbles at high pH, thus enhancing interface adsorption and degradation, as well as by the high ionic strength of the solution helping to generate ultrafine bubbles and maintaining them through ionic shielding. The current work provides useful insights into the application of microbubble as a promising technique.  相似文献   
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