玉米秸秆消化率全基因组关联分析

48h体外干物质消化率（48h in vitro dry matter digestibility, 48h IVDMD）是衡量青贮玉米品质的重要指标。为了初步探究玉米秸秆消化率的分子遗传机理,以341份玉米自交系为材料,于2018年在沈阳和通辽种植,收获后测定秸秆48h IVDMD。利用全基因组重测序获得的6 276 612个高质量SNPs进行全基因组关联分析,共检测到153个与玉米秸秆消化率显著相关的SNPs位点（P<1.0×10^-6）,4个SNPs显著水平在P<1.0×10^-8以上;共找到38个秸秆消化率的候选基因,主要涉及细胞生长发育、防御反应和信号转导等生物学功能。     

Genome-wide association study for spot blotch resistance in Afghan wheat germplasm

Spot blotch (SB), caused by Bipolaris sorokiniana, is a devastating disease of wheat globally, especially in South Asia and South America. Understanding the genetics of resistance to SB is important for developing breeding strategies to improve resistance. A panel of 301 genotypes from Afghanistan was phenotyped over two crop seasons using a mixture of virulent B. sorokiniana isolates and genotyped using DArTSeq to obtain genome-wide markers. Fifty genotypes (16.6%) showed disease scores less than the resistant control. Principal component analysis using the genotypic data clustered the genotypes into five different groups. Among models used for genome-wide association mapping, the multilocus mixed model, and fixed and random model circulating probability unification algorithms were most effective in identifying significant marker-trait associations (MTA). Twenty-five MTAs at p ≤ .001 were identified on chromosomes 1A, 1B, 1D, 2B, 2D, 3A, 3B, 4A, 5A, 5B, 6A, 7A, and 7D, indicating the quantitative nature of resistance to SB. Phenotypic variation explained by these markers ranged from 2.0% to 17.7%, and genomic regions on the chromosomes 1D, 2D, 3A, 3B, 4A, 5A, and 5B coincided with loci identified in previous studies. Three single nucleotide polymorphism (SNP) markers on chromosomes 1B (SNP 1113207) and 5A (SNPs 5411867 and 998276) were significant in both crop seasons as well as in the combined analysis across seasons. Marker 5411867 is close to Vrn-A1, shown to be associated with SB in previous studies. Furthermore, among known SB resistance genes, Sb2 on chromosome 5B was predicted to be significant in this panel.     

普通小麦主要农艺性状的全基因组关联分析

为解析小麦复杂农艺性状的遗传机制,本研究以150份小麦品种(系)为自然群体,在4个环境条件下测定了9个主要农艺性状,利用小麦35K SNP芯片,结合5种关联模型(Q、PCA、K、PCA+K、Q+K),进行全基因组关联分析。结果表明,全基因组多态性信息量PIC的范围为0.0950～0.5000,最小等位基因频率MAF值为0.0500～0.5000;群体结构分析和PCA分析均表明参试材料可分为两个亚群;连锁不平衡分析发现A基因组、B基因组、D基因组和全基因组的LD衰减距离分别为4.7、8、11和6 Mb。9个性状共检测到652个显著的关联位点(P≤0.001),其中21个SNP在2个或2个以上的环境中被重复检测到,分布在1A(1)、1B(4)、2A(3)、2D(2)、3A(1)、5A(1)、5B(5)、6A(1)、6B(2)和7D(3)染色体上; 1个SNP标记的物理位置未知, 3个SNP标记同时与2个性状显著关联;单个SNP的表型贡献率为7.67%～18.79%。8个优势等位变异在供试群体中所占比例较低,筛选出14个可能与小麦农艺性状相关的候选基因,其中TraesCS5B02G237200、TraesCS7D02G129700和TraesCS1B02G426300可能在植物抵御生物与非生物胁迫中起作用,TraesCS5B02G010800和TraesCS7D02G436800可能与植物激素的合成和响应有关,TraesCS2A02G092200可能与植物细胞壁的增强有关, TraesCS5A02G438800可能参与叶绿体发育,另外7个候选基因的功能未知。     

含刚性沉水植物明渠水流结构的试验研究

【目的】得到含刚性沉水植物明渠的水流结构。【方法】采用粒子图像测速仪(PIV),用有机玻璃棒模拟刚性植物,在不同来水流量、植物密度条件下对明渠水流结构进行了试验研究。【结果】无植物时平均流速沿垂向呈对数分布规律,有植物时则呈现明显的分区分布特性。无植物时紊动强度值沿垂向变化不大,有植物时在植物顶端位置处紊动最剧烈。流量或植物密度越大,植物顶端位置的流速梯度就越大,植物层上方的流速最大值也越大。植物密度越大,植物顶端位置的紊动强度越大,植物密度对水流紊动强度由最大值减小到最小值的区域影响很大。【结论】刚性沉水植物的存在会改变水流结构,增强紊动掺混,增强流体质点交换和能量传递,且上述影响会随着流量或植物密度的增大而增强。     

Hybrid breeding for biomass yield in winter triticale: II. Combining ability and hybrid prediction

Accurate hybrid prediction and knowledge about the relative contribution of general (GCA) and specific combining ability (SCA) are of utmost importance for efficient hybrid breeding. We therefore evaluated 91 triticale single-cross hybrids in field trials at seven environments for plant height, heading time, fresh biomass, dry matter content and dry biomass. Fresh and dry biomass showed the highest proportion (23%) of variance due to SCA. Prediction accuracies based on GCA were slightly higher than based on mid-parent values. Utilizing parental kinship information yielded the highest prediction accuracies when both parental lines have been tested in other hybrid combinations, but still moderate-to-low prediction accuracies for two untested parents. Thus, hybrid prediction for biomass traits in triticale is currently promising based on mid-parent values as emphasized by our simulation study, but can be expected to shift to GCA-based prediction with an increasing importance of GCA due to selection in hybrid breeding. Moreover, the performance of potential hybrids between newly developed lines can be predicted with moderate accuracy using genomic relationship information.     

环磺酮原药中3个杂质结构的确定及其合成

采用文献中报道的环磺酮的合成工艺合成了环磺酮原药，通过高效液相色谱分析，发现其原药中含有3个质量分数分别大于0.1%的杂质；通过核磁共振氢谱 (1H NMR) 及高分辨质谱 (HRMS) 对3个杂质的结构进行了确证，同时进行了合成。根据合成工艺路线，对该杂质产生的途径进行了分析。     

储料竖向压力对粮仓中小麦粮堆湿热传递的影响

粮仓中存在压力场、温度场和湿度场等多物理场,为了得出各物理因子共同影响下的粮堆内湿热传递规律,该研究利用自行研制的粮堆多场耦合试验装置,针对仓内小麦粮堆单元体,研究在高温边界38.5℃、低温边界5.2℃,初始粮温25.8℃,竖向压力分别为50、100、150 kPa条件下小麦粮堆湿热传递情况。试验结果表明:竖向压力增加,粮堆孔隙率减小,热量通过粮食籽粒间传导增加,传递速率加快,竖向压力从50 kPa增大至150 k Pa,粮温较入仓时下降约0.5~1.3℃,温度梯度变化率达8.7%,不同压力下粮堆高温区面积随储藏时间呈幂函数减小。粮堆内湿空气在边界处累积至峰值时会有部分湿空气向粮堆内迁移。粮堆中部与靠近低温边界温差大于6.3℃时,粮堆内湿空气扩散加快,粮堆中部平均相对湿度下降速率随竖向压力增加而加快。研究结果可为散装粮堆多场耦合研究提供理论支持。     

非洲猪瘟疫情对生猪产业链上市公司市场价值的影响研究

[目的] 研究旨在应用实证方法分析非洲猪瘟疫情对生猪产业链上市公司市场价值造成的影响,进而得出疫情对生猪产业链上养殖、屠宰、加工、消费等环节利益相关者收益的影响。[方法]采用事件研究法,选取涉及生猪产业链上市公司,按主营业务统计为肉制品加工、养殖、饲料和兽药4种类型的32家相关上市公司,通过设计指标对生猪产业链上市公司股票异常收益率波动进行计算和检验。[结果]研究结果表明：非洲猪瘟疫情事件对生猪养殖行业的影响最大；对兽药行业的影响周期最长；肉制品加工行业的影响程度和周期仅次于兽药行业,对饲料行业的影响最小。[结论] “非洲猪瘟”事件对猪产业链上的饲料、养殖、肉制品加工、兽药类上市公司市场价值存在显著的负面影响。