我国大蒜产业现状及发展对策

大蒜是一种经济作物。我国大蒜种植历史悠久,目前以大蒜产业为主要收入的农户有千万人以上;在国际市场上,我国大蒜占据超过60%的份额,是世界上最大的大蒜生产国和消费国。然而,随着经济的发展,大蒜产业在发展过程中逐渐暴露出许多问题。本文针对我国大蒜产业的现状和问题,提出了解决对策,为我国调整大蒜产业发展结构提供理论依据。     

中国大蒜主产区主要品种鳞茎品质及其与产地土壤养分的关系

以全国16个省区50个品种大蒜与对应土壤为材料,分析大蒜鳞茎中大蒜素、硒元素、锗元素、可溶性糖、可溶性蛋白的含量,测定蒜瓣数、蒜头质量等7个鳞茎性状及土壤化学性状指标,通过主成分分析对鳞茎性状进行综合评价,分析大蒜鳞茎与产地土壤化学性质的相关性。结果表明:大蒜素、硒、锗和可溶性糖4个主成分累计方差贡献率达到79.8%,反映大蒜品种鳞茎品质的基本信息。鳞茎营养品质大蒜素、硒、锗、可溶性糖、可溶性蛋白及表型品质蒜瓣数、蒜头质量综合排序,品质较好的(F≥0.5)品种是‘苏02’‘苏01’‘贵02’‘豫07’和‘晋01’,品质一般(0≤F0.5)的品种19个,品质较差(F0)的品种超过一半。相关分析表明,土壤养分含量与大蒜品质间的相关性较小,只有硫元素与大蒜素和可溶性糖显著正相关,土壤pH与大蒜锗含量显著正相关,土壤N含量与大蒜可溶性糖极显著正相关。     

原子吸收光谱法检测大蒜制品中微量元素锗

[目的]研究不同大蒜制品中锗元素含量的差别。[方法]采用马弗炉灰化样品,火焰原子吸收光谱法测定以金乡紫皮蒜为原料加工而成的黑蒜、蒜蓉辣酱、脱水蒜片、蒜粉、糖醋蒜5种制品中锗的含量。[结果]试验表明,黑蒜中锗含量为1.384 mg/g,蒜蓉辣酱中锗含量为0.784 mg/g,脱水蒜片中锗含量为0.913 mg/g,蒜粉中锗含量为0.956 mg/g,糖醋蒜中锗含量为0.827 mg/g。5种蒜制品中均含有锗元素,其含量由多到少依次为黑蒜、蒜粉、脱水蒜片、糖醋蒜、蒜蓉辣酱。[结论]研究可为大蒜及大蒜制品的开发利用提供参考依据。     

陕西兴平白皮蒜挥发油化学成分的分析

以陕西兴平白皮蒜为试材，采用水蒸气蒸馏—乙醚萃取法提取挥发油，用气相色谱/质谱法分析了大蒜挥发油的化学成分。共分离得到33个组分，鉴定出26个化学组分，占挥发油总量的99.39 %。兴平白皮蒜挥发油中的化学成分以含硫化合物为主，共有18个，占挥发油总量的98.80%。兴平白皮蒜挥发油主要成分为：大蒜新素(二烯丙基三硫化物，43.85%），大蒜素（二烯丙基二硫化物，35.28%）和甲基烯丙基三硫化物（11.10%）。     

大蒜茎尖脱毒技术及组织培养研究

采用山西栽培的５个地方名蒜茎尖离体培养，获得了无病毒蒜种。结果表明，茎尖大小为０．２￣０．５ｍｍ，不定芽增殖培养基以Ｂ５或ＭＳ４，附加１￣２ｍｇ／Ｌ细胞分裂素和０．０１￣０．１ｍｇ／ＬＮＡＡ，效果最佳。根系诱导在原培养基中加入０．２ｍｇ／Ｌ ＮＡＡ，出根率可达９０％以上。成苗后于１２月份移栽到节能日光温室，成活率达９０％以上。经对脱毒苗形态观察和染色体检测，没有发生异常变化。植株生长健壮，遗传性稳     

大蒜发芽叶愈伤组织细胞超微结构观察与过氧化物酶同工酶的变化

在大蒜发芽叶愈伤组织发生过程中,线粒体、内质网、核糖体和质体的数量呈增加趋势,结构呈完善趋势,细胞液泡化程度降低;而愈伤组织细胞中,这些细胞器的数量有所下降,有的线粒体发生解体.细胞脱分化过程中,细胞分裂以核缢裂的无丝分裂方式进行.在愈伤组织发生过程中,过氧化物酶同工酶谱呈减少和减弱的趋势,而在愈伤组织中,又有所增加和增强.     

蒜薹采后致病菌种类及侵染规律研究

以金乡蒜薹和苍山蒜薹为试材,研究蒜薹采后病原菌种类、侵染和发病规律。结果表明:导致蒜薹采后贮藏过程中腐烂的病原真菌分为9个属14个种,其中主要是交链格孢属(Alternaria)占28.5%,并且,交链格孢属中,链格孢霉(A.alternate(Fr.)Keissler)最多,占17.9%;其次是葱紫斑交链孢霉(A.porri(Elliott)Cif.),占6.6%,侵染链格孢霉(A.infectoriaSimmons)占4.0%;葡萄孢属(Botrytis),占17.0%,主要是葱鳞葡萄孢霉(B.squamosaWalker)和葱腐葡萄孢霉(B.alliiMunn),占15.6%,灰葡萄孢霉(B.cinereaPers)仅为1.4%;而青霉属(Penicillium)、匐柄霉属(Stemphylium)、镰孢属(Fusarium)、芽枝霉属(Cladosporium)、腐霉属(Pythium)、疫霉属(Phytophthora)和聚端孢霉属(Trichothecium)依次占15.7%、12.6%、7.8%、6.5%、4.9%、3.8%和3.3%。其发病规律是:除青霉属外,其它12种病原菌初期主要侵染蒜薹的薹梢部位,之后再逐渐向薹梗、薹基部位蔓延。     

氮钾配施对大蒜增产效应的研究

1999～2001年连续三年多点试验结果表明:在安徽省来安县大蒜产区水稻土上氮钾配施对大蒜产量及品质都有明显的正效应,蒜苔增产24.2%～29.2%,蒜头增产17.3%～18.9%。氮钾肥的产量效应与土壤的氮钾水平和氮钾比例密切相关。氮肥的适宜用量为N300～375kg/hm2,钾肥为K2O150kg/hm2。回归方程计算显示,大蒜产区的土壤临界钾素含量为101μg/ml,氮素临界含量为46μg/ml(ASI法测定)。氮钾配施并能提高蒜苗、蒜苔维生素C及可溶性糖含量。增施钾肥对大蒜的经济效益明显提高,产投比为11.07～13.26。     

CHANGES IN GROWTH AND GENE EXPRESSION INDUCED BY SULFUR DEFICIENCY IN GARLIC

ABSTRACT

Sulfur deficiency in garlic Allium sativum L. caused a reduction in growth together with chlorosis and necrosis of leaves. Large differences in shoot sulfur and sulphate concentrations between deficient and high sulfur treatments were only observed after 54 days growth. Using the mRNA differential display technique, a novel cDNA was isolated from shoots grown in sulfur depleted nutrient solution for 24 days. This novel cDNA was constitutively expressed in the shoots during further growth in sulfur depleted solution, but it was undetectable following 30 days recovery with sulfur supplementation. The cDNA sequence demonstrated a high degree of identity with a coat protein gene of a garlic latent carlavirus. The results suggest a possible relationship between low plant sulfur status and the induction of a latent carlavirus in garlic.     

Manipulation of rumen fermentation and ecology of swamp buffalo by coconut oil and garlic powder supplementation

This experiment was conducted to investigate the effect of coconut oil and garlic powder supplementation on digestibility of nutrients, rumen fermentation, rumen ecology, rumen microorganism and methanogen diversity. Four, 3-year old, rumen fistulated swamp buffalo bulls were randomly assigned in a 4 × 4 Latin square design to receive four dietary treatments; 7% coconut oil, 7% coconut oil with 50 g/day of garlic powder, 7% coconut oil with 100 g/day of garlic powder and non-supplemented (control). During the experiment, concentrate was offered at 0.5% of BW while rice straw was given on ad libitum basis. It was found that supplementation of 7% coconut oil had significantly influenced on total DM intake, OM, NDF and ADF digestibilites while supplementation of 7% coconut oil with garlic powder (50 and 100 g/day) were not significantly different when compared with the control. Dietary supplementations did not affect on rumen pH, NH₃–N concentrations. Blood urea nitrogen (BUN) was significantly higher in supplemented groups. Total VFA concentration, proportion of acetate and acetate to propionate ratio were reduced by supplementation. Proportion of propionate was increased (P < 0.05) when supplemented with 7% coconut oil and 7% coconut oil with 100 g/day of garlic powder. Methane production was dramatically reduced (P = 0.005) in supplemented treatments and was 10% reduced in 7% coconut oil supplementation. Amylolytic and proteolytic bacteria were increased (P = 0.007 and P = 0.024) while protozoal population decreased by 68–75% (P < 0.01) by supplementation. Total bacterial population was increased by supplementation while total fungi and total methanogens were not significantly different among treatments. Percentage of cellulolytic bacterial population was not different among treatments. However, dietary supplementation reduced (P < 0.001) percentage of F. succinogenes population. However, methanogen diversity was not changed using PCR–DGGE as technique. Thirteen separate bands were found in the DGGE gel. Based on this study, supplementation with 7% coconut oil plus 100 g/day of garlic powder could be efficiently utilized in the rumen and thus, could provide good fermentation end products and improve rumen ecology for the host swamp buffaloes particularly in reducing 9% methane gas production without changing nutrient digestibilities.