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A Rapid Method for Measuring Freezing Resistance in Crop Plants   总被引:3,自引:0,他引:3  
The objective of this study was to develop a technique based on chlorophyll fluorescence to assess freezing injury and resistance of leaves. Optimization was done with faba bean leaves and applicability to other crops was examined at winter and spring with types of barley, oats, rape and faba beans. Selected leaves from young hardened beans were subjected to standardized freezing tests with different minimum temperatures ( T min) and fluorescence was monitored. After a dark period basic fluorescence ( F O was induced by 0.2 μmol m−2 s−1 pulsed red light and maximum fluorescence ( F m) was assayed at different light intensities. 1500 μmol m−2 s−1 rendered to give the maximum possible output of Fm and best differentiation of differently damaged leaves by F n= F m - F O. Leaf temperature during measurement and during a short storage (± 2 h) should be kept at about 0°C to avoid biases between differently damaged leaves. The measuring spot on the leaf must be standardized since fluorescence response differed at the tip and base of a leaflet, but not between the two leaflets of a faba bean leaf. The applicability of F rr (ratio of F r of stressed to unstressed leaves) as a measure of resistance was demonstrated by comparison of winter hardiness of cultivars with freezing resistance calculated from the relationship of F vr and the T min used in freezing tests.  相似文献
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低温锻炼对草莓组培苗抗寒性及抗氧化酶活性的影响   总被引:3,自引:2,他引:1  
草莓组织培养苗经4℃低温锻炼后抗寒性明显增强,SOD、CAT、POD和APX活性明显提高,可溶性蛋白质、脯氨酸和可溶性糖等渗透调节物质含量增加。同时,低温锻炼对膜造成了一定程度的伤害,表现为MDA含量的增加。脱锻炼后抗氧化酶活性和可溶性蛋白质、脯氨酸、可溶性糖及MDA含量略有下降,但仍高于未锻炼植株。这些变化是草莓组织培养苗适应低温逆境的生理生化基础。  相似文献
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甜杨抗冻转录因子ICE1基因的in silico克隆及其分析   总被引:2,自引:0,他引:2  
ICEl基因编码类似MYC的bHLH转录因子,可特异地结合到CBF3启动子的MYC作用元件并诱导CBF/DREB1下游基因的转录表达.本文采用电子克隆的方法,以拟南芥ICE1蛋白序列为信息探针,利用杨树EST数据库和毛果杨基因组序列拼接的结果,设计引物并通过RT-PCR从甜杨克隆了杨树的第一个ICE1基因.其cDNA长1 706 bp,含有完整的开放阅读框,可编码543个氨基酸的MYC类蛋白.编码蛋白序列含有bHLH区,核定位信号(NLS)区,富S区和转膜区各1个.Blast分析表明,cDNA序列及其推导的氨基酸序列均与拟南芥和芥菜的ICE1存在着较高的同源性,说明所获得的cDNA可能是甜杨ICE1基因(PsICE1,DQ481236).通过网络服务器平台进行PsICE1的功能预测,结果显示PsICE1含有bHLH保守功能域,具有多个磷酸化位点和跨膜区域.另外,ICE1的电子表达谱分析结果发现,ICE1几乎可在植物中整株表达,在多种组织和不同发育过程均表达,这也在一定程度上说明了ICE1是组成型表达,以及ICE1可能在植物的生长发育中也起着重要作用.  相似文献
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澳大利亚苜蓿抗寒性研究进展   总被引:1,自引:0,他引:1       下载免费PDF全文
欧洲和美国的苜蓿品种在恶劣的生长环境下持久性较差,因此培育能适应澳大利亚严酷的生长条件的苜蓿品种非常关键。通过引进优良苜蓿品系和新搜集的苜蓿品系进行杂交,获得理想杂交品种,在多种不同的气候区域及土壤类型下对杂交种抗寒性进行测定,保留表现最好的杂交后代,形成前基础种子。在国内外大量研究成果基础上,结合澳大利亚苜蓿品种选育,系统阐述了苜蓿在低温胁迫下,其形态、生理、生化诸方面适应性变化以及秋眠性与抗寒性的关系,并对苜蓿抗寒基因工程研究进展作了相关报道  相似文献
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Quinoa (Chenopodium quinoa Willd.) is traditionally grown in the mountain regions of the Andes where frost is common. However, the physiological mechanisms responsible for the frost resistance observed in quinoa are largely unknown. For this reason, a study on cultivars of quinoa originating from the Andean highlands and from the inter-Andean valleys was performed. Frost tolerance was determined by measuring the average lethal temperature of 50% of the leaf tissues (LT50) by ion leakage, and supercooling activity was assessed by thermal analysis using thermocouples. Quinoa demonstrated supercooling capacity (a mechanism that prevents immediate damage by freezing temperatures) of 5 °C. Ice nucleation temperature was always lower than the LT50. This indicates that the main survival mechanism of quinoa to frost is avoidance of ice formation by moderate supercooling. The study revealed that quinoa has a high soluble sugar content, which may cause a lowering of the freezing point and therefore contributing to lower the LT50. It is suggested that the content of proline and soluble sugars, such as sucrose, may serve as indicators of frost tolerance in quinoa breeding material.  相似文献
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为了验证昆明市2013年12月所遭受的“10年1遇”的极端低温天气对该市种植的榕属植物的影响,笔者对市内最常见的3种榕属植物小叶榕、垂叶榕和高山榕所遭受的冻害情况进行调查和分析,将每种处理的冻害程度换算为冻害指数并进行统计分析,并对这3种榕树的冻害恢复进行了2年跟踪调查。结果表明:微环境对榕树的冻害具有显著影响,附近有遮挡的榕树更不容易被冻伤,同时,市郊的榕树比市中心的榕树更容易遭受低温冻害,而榕树的直径对冻伤程度并无显著影响。经过2年的恢复,几乎所有的榕树都能够逐渐恢复健康。结果说明,榕树在昆明市虽可能会遭受低温冻害,但经过后期的护理和恢复,基本都不会死亡,但在郊区,特别是郊区空旷地带,冻害较为严重,需谨慎选择榕树作为行道树。  相似文献
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