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1.
为了探究干扰素调节因子2 (interferon regulatory factor,IRF2)如何通过调控干扰素(IFN)表达影响鱼类的免疫,实验从青鳉中克隆了irf2 (Olirf2),发现该基因在青鳉各个组织中均有表达;将构建的真核表达载体pTol2/CMV-IRF2/IE1-pr转染到胖头鱥肌肉细胞系(FHM)后,发现瞬时过表达Olirf2能够显著促进鲤春病毒血症病毒(spring viremia of carp virus,SVCV)的复制,并抑制抗病毒相关基因mx1、ifn和irf3的表达。进一步通过双荧光素报告系统发现,Olirf2能够显著抑制NF-κB和ISRE的活性,说明Olirf2可能通过抑制细胞的天然免疫应答进而促进病毒的增殖。然而持续过表达Olirf2则增强了细胞的抗病毒能力,同时促进干扰素相关基因mx1、ifn和irf3的表达。因此,Olirf2基于表达的持续时间不同而具有抗病毒或者促病毒的双面效果。实验通过研究Olirf2在抗病毒信号通路中发挥的作用,为通过基因编辑或者转基因手段来构建抗病毒的鱼类提供了理论基础。  相似文献   
2.
王言聿  郭旭  成志强 《油气储运》2021,(2):215-221,227
与传统管线钢管相比,复合材料增强管线钢管(CompositeReinforcedLinePipe,CRLP)具有承压能力高和质量轻等优势,但对其压力设计的研究尚不充分。CSAZ662-2015《油气管道系统》和GB/T35185—2017《石油天然气工业用复合材料增强管线钢管》中最大许用操作压力的设计思路不同,且设计结果差别较大。针对此问题,基于组合薄壁圆筒模型对承压CRLP进行分析,对最大许用操作压力下材料的名义许用应力和真实许用应力进行了区分,进而导出真实许用应力的显式表达形式,并以此作为设计安全裕度的评价指标。分析表明:现有规范和标准中的设计公式均无法保证管线钢和复合材料的真实许用应力为常量,对应两种设计方法,各组分材料的真实许用应力均随CRLP规格差异而发生变化,并且随着试压/预应力处理的历程而改变。基于上述分析和CRLP极限状态下的承载特征,提出一种改进的压力设计方法。新方法优先保证复合材料的真实许用应力为常量,并可由设计系数直接确定;在最大许用操作压力的计算中,考虑了加载历程的影响,物理意义明确。算例表明,其设计安全裕度较现有方法更为合理,对于CRLP压力设计的改进有参考价值。  相似文献   
3.
以X波段双偏振雷达的多种参数为依据,综合使用雷达及相关雨量站的降水观测资料,从基本组成及雷达工作方式2 个方面简述了X波段双偏振多普勒雷达,并针对2014 年8 月9 日内蒙古地区的一次对流性降水天气过程,着重分析X波段双偏振多普勒雷达降水观测。结果表明:(1)在流性雷暴云降水过程中,大部分冰晶类将会在6~8 km融化为小雨滴,或以冻滴的形式存在;(2)强雷暴云天气发生时,在强雷暴云中上层的存在大部分的小粒子,并无大量的水成物粒子存在。该研究不仅能够探索雷达对降水进行定量估测的能力,还能够通过提升X波段双偏振雷达降水观测的适用性,以期为今后X波段双偏振多普勒雷达在降水观测中的应用提出一定的参考依据。  相似文献   
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5.
Overweight and obesity are multifactorial diseases caused by an imbalance in energy metabolism. An underlying genetic predisposition is often a factor in these conditions. In the cat breeding family of the Institute of Animal Nutrition at the Vetsuisse Faculty, University of Zurich, a segregating overweight phenotype with a genetic contribution was observed. From this breeding family, 26 kittens were followed from birth up to 8 months of age. During this time, food intake was measured using an automatic feeding station, and energy expenditure was investigated using indirect calorimetry at the ages of 4 and 6 months. Dual‐energy X‐ray absorptiometry (DEXA) was performed and blood glucose, leptin and insulin were measured at the ages of 4, 6 and 8 months. The kittens were also weighed daily for the first 2 weeks of life, every second day until weaning and once per week until 8 months of age. The body condition score (BCS) was evaluated monthly between 2 and 8 months of age. The main finding of this study is that a predisposition to overweight is connected to a higher food intake early in life, with no significant alterations in energy expenditure. The leptin blood levels were related to body fat percentage, and insulin sensitivity did not seem to be affected.  相似文献   
6.
The newly formed extensive flooded land associated with the Three Gorges Reservoir hold great potentials to provide ecosystem services (ESs) for surrounding cities and communities. Conflicting values and conventional management strategies may complicate and inhibit broad-scale decision-making for this novel ecosystem. Public attitudes are a crucial influence in the way this process plays out. This study was conducted to assess how the reservoir bank is perceived by the general public of Chongqing Municipality in terms of importance of riparian ESs, and preference for corresponding landscape design styles through photo-questionnaire interviewing. Although Chongqing citizens know little of the “ecosystem services” concept, they do recognise riparian environmental and ecological benefits especially the bank protection and pollution interception functions, while benefits of habitats for wildlife and flood attenuation were of less concern. Recreational values were least appreciated, which is inconsistent with other studies. This may be attributed to a wider, intensive environmental education in various social media in today’s China. But publicity has been dominated by toxic air, water and soil pollution control rather than the benefits of nature conservation and personal contact with nature. In line with recognition of ESs, there is quite strong preference for the forested design scenario and particularly vegetation components across the socio-demographic spectrum. Overall, socio-demographic variables have very low explanatory power for the perceptual responses. Our study also revealed misunderstandings around riparian functions and a gap between public expectations and special abiotic and biotic prerequisites in restoring novel ecosystems. Finally, we discuss the relevance of this research to urban novel ecosystems in general, and implications for raising public awareness, engaging appropriate public values in sustainable design and management of such ecosystems.  相似文献   
7.
农田边界植物多样性与生态服务功能研究进展   总被引:1,自引:0,他引:1  
农业集约化和城市化导致农业景观重要生境发生巨大变化,农田野生动植物数量下降。农田边界作为农业景观中重要的组成部分,其丰富的物种组成,不仅具有重要的生态功能,也具有重要的景观价值。本研究对农业生态系统农田边界植物多样性进行阐述,论述了影响农田边界植物多样性的影响因素和生态服务功能。综述研究显示,农田边界具有类型多样性、物种多样性、植物功能类型多样性及时空结构多样性特征;其影响因素主要包括农田边界结构、农田边界管理、周边土地利用影响以及景观结构特征的影响4个方面;农田边界生态功能包括生产、环境保护、野生动植物保护和景观美学功能等;最后总结了不同类型农田边界生态植被营建模式与优化途径,对中国农田边界植被管护提出了一些建议。  相似文献   
8.
In order to alleviate poverty in Northeast Thailand, the Thai government has promoted rubber farming, which has expanded at the expense of annual crops. Because of a long immature period, planting rubber represents a loss of income for poor farmers in the very first years. This paper analyzed how rubber intercropping during the immature period helps farmers to compensate for this loss of income. Economic performances of the most widespread rubber farming systems were analyzed using information collected from a questionnaire addressed to 35 farmers in Buriram province. A sub-sample of 22 farmers was further interviewed to estimate the contribution of rubber intercropping in the formation of the total annual income during the immature period. The results showed that interest in rubber intercropping has grown, with cassava and rice as the main associated crops. With additional costs of about 14,169 ?/ha/year over monospecific rubber plantations, rubber-cassava intercropping systems generated a gross margin estimated at 11,340 ?/ha/year for a 3-year period. Compared to a monospecific rubber plantation, rubber-cassava intercropping systems reduced management costs by 59% over the 6-year period of rubber immaturity. The cash-income drawn from intercropping ranged from 0 to 26.8% of the household’s total annual income, which can be of considerable importance for low-income farmers.  相似文献   
9.
基于修正双作物系数模型估算温室黄瓜不同季节腾发量   总被引:6,自引:5,他引:1  
为估算温室黄瓜植株蒸腾与土面蒸发,该研究基于FAO-56推荐的双作物系数模型,应用温室内实测微气象、叶面积指数(LAI)及土壤水分数据,对模型中基础作物系数(Kcb)和土面蒸发系数(Ke)进行修正,并基于修正后FAO-56Penman-Monteith(P-M)模型,确定温室参考作物蒸发蒸腾量(ET0),进而估算温室黄瓜蒸发蒸腾量(ETc)和植株蒸腾(Tr)。基于Venlo型温室内黄瓜不同种植季节(春夏季和秋冬季)Lysimeter和茎流计观测的黄瓜ETc和Tr,对修正后的双作物系数模型预测结果进行验证。结果表明,应用修正后的双作物系数模型估算的温室黄瓜ETc和Tr与实测值具有较好地一致性,春夏季温室黄瓜全生育期ETc估算值与实测值的日均值分别为3.05和2.94 mm/d,秋冬季分别为2.53和2.76 mm/d。修正后的双作物系数模型估算春夏季温室黄瓜日ETc的决定系数(R2)、均方根误差(RMSE)和模型效率系数(Ens)分别为0.95、0.41 mm/d和0.93;估算秋冬季ETc的误差计算结果依次为0.91(R2)、0.48 mm/d(RMSE)和0.90(Ens)。修正后的双作物系数模型估算春夏季日平均Tr与实测值分别为2.37和2.19mm/d,秋冬季分别为1.43和1.34 mm/d。研究结果还显示,不同种植季节温室黄瓜全生育期日平均Tr占ETc的比例分别为64.62%(春夏季)和68.59%(秋冬季)。该研究成果不仅为制定准确的温室黄瓜灌溉制度提供了理论依据,而且对实现温室环境智能化控制及减少温室内无效的土面蒸发具有重要意义。  相似文献   
10.
金边虎尾兰的金边属于嵌合体彩斑,叶插繁殖易使其金边消失。根据相关文献和实践经验,介绍了保持金边虎尾兰金边不消失的4种繁殖方法,其中根状茎扦插是一种新的繁殖方法。  相似文献   
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